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Reviewed, UniProtKB/Swiss-Prot Q12066 (YDL89_YEAST)

Last modified June 16, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Uncharacterized membrane protein YDL089W
Gene names
Ordered Locus Names: YDL089W
ORF Names: D2416
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Subunit structure

Interacts with CSM1. Ref.4

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

Present with 606 molecules/cell in log phase SD medium. Ref.3

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Ontologies

Keywords
   Cellular componentMembrane
   DomainTransmembrane
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmaintenance of rDNA

Inferred from mutant phenotype. Source: SGD

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

nuclear periphery

Inferred from direct assay. Source: SGD

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484Uncharacterized membrane protein YDL089W
PRO_0000202586

Regions

Topological domain1 – 5959Extracellular Potential
Transmembrane60 – 8021 Potential
Topological domain81 – 11838Cytoplasmic Potential
Transmembrane119 – 13921 Potential
Topological domain140 – 1412Extracellular Potential
Transmembrane142 – 16221 Potential
Topological domain163 – 23876Cytoplasmic Potential
Transmembrane239 – 25921 Potential
Topological domain260 – 2612Extracellular Potential
Transmembrane262 – 28221 Potential
Topological domain283 – 484202Cytoplasmic Potential

Amino acid modifications

Modified residue31Phosphoserine Ref.7
Modified residue4161Phosphoserine Ref.7
Modified residue4171Phosphoserine Ref.7
Modified residue4391Phosphoserine Ref.6
Modified residue4411Phosphoserine Ref.6

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Sequences

Sequence LengthMass (Da)Tools
Q12066-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3785895EA2887E89

FASTA48456,887
        10         20         30         40         50         60 
MGSNDLINEA YDDSEVVGEE RESKSAWMKR WYQLLTSPLD LQLVINEKLE MINWDAYAKS 

        70         80         90        100        110        120 
LAKPLGNFLT ILFFIIRLLQ DNLIKPNYYK LNVKSGAFDL SKSNKLKEFD YLWEISSSFQ 

       130        140        150        160        170        180 
NNNQFYAFQS WYFVTLRFLN NLFRFTIFIL LSLNLYVSCK FMFGYFKTYN LFHLKKEFNS 

       190        200        210        220        230        240 
PNLTKHNLKD LSKEYYEDIY KQSLWSMLKH FFRGSRDDGP HVNQNEDEIF FQLRKWIPTN 

       250        260        270        280        290        300 
FMINLFVSFS PTAIVFLSFS DVSFTSAIAI VFHQYILDYI ITKRFQRSVD DDLILSSAAL 

       310        320        330        340        350        360 
QEYEDKHIMA RINQCSNIDT LSSAMGTRSK TPRIFTTHSL CGEEIREVYN YEKREFEALP 

       370        380        390        400        410        420 
KMTESVPGSR ETRIKDYGGI SQVSDHQSHP IGFHYSPRMS PYYRDKVLDN NLAQSSSNEN 

       430        440        450        460        470        480 
LEKGGAYLPN QDQNRPSKSL SPLRKTPLSA RQKRFEGSEF NVLNKNDINS ILRSPKKKKN 


YHKR

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References

« Hide 'large scale' references
[1]"The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames."
Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A.
Yeast 12:1377-1384(1996) [PubMed: 8923743] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]"Saccharomyces cerevisiae CSM1 gene encoding a protein influencing chromosome segregation in meiosis I interacts with elements of the DNA replication complex."
Wysocka M., Rytka J., Kurlandzka A.
Exp. Cell Res. 294:592-602(2004) [PubMed: 15023545] [Abstract]
Cited for: INTERACTION WITH CSM1.
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[6]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-439 AND SER-441, MASS SPECTROMETRY.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3; SER-416 AND SER-417, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X95644 Genomic DNA. Translation: CAA64922.1.
Z74137 Genomic DNA. Translation: CAA98655.1.
PIRS67625.
RefSeqNP_010194.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1799N.
IntActQ12066. 10 interactions.

Genome annotation databases

EnsemblYDL089W. Saccharomyces cerevisiae. [Contig view]
GeneID851469.
GenomeReviewsGene locus YDL089W in contig Z71256_GR.
KEGGsce:YDL089W.
NMPDRfig|4932.3.peg.932.

Organism-specific databases

CYGDYDL089w.
SGDS000002247. YDL089W.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMQ12066.
OMAQ12066. QDAMVDA.

Gene expression databases

ArrayExpressQ12066.
GermOnlineYDL089W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR018819. DUF2418.
[Graphical view]
PfamPF10332. DUF2418. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio968759.

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Entry information

Entry nameYDL89_YEAST
AccessionPrimary (citable) accession number: Q12066
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents