Q12066 (NUR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear rim protein 1

Gene names

Name:	NUR1
Ordered Locus Names:	YDL089W
ORF Names:	D2416

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of a perinuclear network that controls recombination at multiple loci to maintain genome stability. Required for rDNA repeat stability. Ref.9
Subunit structure	Interacts with CSM1. Ref.6 Ref.9
Subcellular location	Nucleus membrane; Multi-pass membrane protein Ref.4.
Miscellaneous	Present with 606 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the NUR1 family.

Ontologies

Keywords
Cellular component	Membrane Nucleus
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	maintenance of rDNA Inferred from mutant phenotype Ref.9. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear periphery Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CSM1	P25651	1	EBI-35877,EBI-22001
ERG6	P25087	1	EBI-35877,EBI-6567
PEX30	Q06169	1	EBI-35877,EBI-31008
RTN1	Q04947	1	EBI-35877,EBI-38020
YOP1	Q12402	1	EBI-35877,EBI-37092

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Nuclear rim protein 1		PRO_0000202586
Regions
	Transmembrane	145 – 165	21	Helical; Potential
	Transmembrane	252 – 272	21	Helical; Potential
Amino acid modifications
	Modified residue	3	1	Phosphoserine Ref.8
	Modified residue	416	1	Phosphoserine Ref.8
	Modified residue	417	1	Phosphoserine Ref.8
	Modified residue	439	1	Phosphoserine Ref.7
	Modified residue	441	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q12066 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3785895EA2887E89	FASTA	484	56,887
	10 20 30 40 50 60 MGSNDLINEA YDDSEVVGEE RESKSAWMKR WYQLLTSPLD LQLVINEKLE MINWDAYAKS 70 80 90 100 110 120 LAKPLGNFLT ILFFIIRLLQ DNLIKPNYYK LNVKSGAFDL SKSNKLKEFD YLWEISSSFQ 130 140 150 160 170 180 NNNQFYAFQS WYFVTLRFLN NLFRFTIFIL LSLNLYVSCK FMFGYFKTYN LFHLKKEFNS 190 200 210 220 230 240 PNLTKHNLKD LSKEYYEDIY KQSLWSMLKH FFRGSRDDGP HVNQNEDEIF FQLRKWIPTN 250 260 270 280 290 300 FMINLFVSFS PTAIVFLSFS DVSFTSAIAI VFHQYILDYI ITKRFQRSVD DDLILSSAAL 310 320 330 340 350 360 QEYEDKHIMA RINQCSNIDT LSSAMGTRSK TPRIFTTHSL CGEEIREVYN YEKREFEALP 370 380 390 400 410 420 KMTESVPGSR ETRIKDYGGI SQVSDHQSHP IGFHYSPRMS PYYRDKVLDN NLAQSSSNEN 430 440 450 460 470 480 LEKGGAYLPN QDQNRPSKSL SPLRKTPLSA RQKRFEGSEF NVLNKNDINS ILRSPKKKKN YHKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames." Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A. Yeast 12:1377-1384(1996) [PubMed: 8923743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Saccharomyces cerevisiae CSM1 gene encoding a protein influencing chromosome segregation in meiosis I interacts with elements of the DNA replication complex." Wysocka M., Rytka J., Kurlandzka A. Exp. Cell Res. 294:592-602(2004) [PubMed: 15023545] [Abstract] Cited for: INTERACTION WITH CSM1.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-439 AND SER-441, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3; SER-416 AND SER-417, MASS SPECTROMETRY.
[9]	"Role for perinuclear chromosome tethering in maintenance of genome stability." Mekhail K., Seebacher J., Gygi S.P., Moazed D. Nature 456:667-670(2008) [PubMed: 18997772] [Abstract] Cited for: FUNCTION, INTERACTION WITH CSM1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95644 Genomic DNA. Translation: CAA64922.1. Z74137 Genomic DNA. Translation: CAA98655.1. BK006938 Genomic DNA. Translation: DAA11769.1.
PIR	S67625.
RefSeq	NP_010194.1. NM_001180148.1.
3D structure databases
ProteinModelPortal	Q12066.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1799N.
IntAct	Q12066. 10 interactions.
MINT	MINT-386289.
STRING	Q12066.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDL089W; YDL089W; YDL089W.
GeneID	851469.
KEGG	sce:YDL089W.
NMPDR	fig|4932.3.peg.932.
Organism-specific databases
CYGD	YDL089w.
SGD	S000002247. NUR1.
Phylogenomic databases
eggNOG	fuNOG09831.
OMA	QDAMVDA.
OrthoDB	EOG4PZNH0.
Gene expression databases
ArrayExpress	Q12066.
Genevestigator	Q12066.
GermOnline	YDL089W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018819. DUF2418. [Graphical view]
Pfam	PF10332. DUF2418. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968759.

Entry information

Entry name

NUR1_YEAST

Accession

Primary (citable) accession number: Q12066
Secondary accession number(s): D6VRQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program