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Protein

Dehydrodolichyl diphosphate synthase complex subunit NUS1

Gene

NUS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

With SRT1 or RER2, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER).1 Publication

Catalytic activityi

(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10-55).1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • dolichol biosynthetic process Source: SGD
  • protein glycosylation Source: SGD

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29578-MONOMER
YEAST:G3O-29578-MONOMER
ReactomeiR-SCE-446199 Synthesis of Dolichyl-phosphate
UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrodolichyl diphosphate synthase complex subunit NUS1Curated (EC:2.5.1.871 Publication)
Alternative name(s):
Di-trans,poly-cis-decaprenylcistransferaseCurated
Nuclear undecaprenyl pyrophosphate synthase 1Imported
Undecaprenyl diphosphate synthase1 Publication
Short name:
UDSCurated
Gene namesi
Name:NUS1
Ordered Locus Names:YDL193WImported
ORF Names:D12391 Publication
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL193W
SGDiS000002352 NUS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei97 – 119HelicalSequence analysisAdd BLAST23

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi372N → H: Loss of function. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002421341 – 375Dehydrodolichyl diphosphate synthase complex subunit NUS1Add BLAST375

Proteomic databases

MaxQBiQ12063
PaxDbiQ12063
PRIDEiQ12063

PTM databases

iPTMnetiQ12063

Interactioni

Subunit structurei

Forms an active dehydrodolichyl diphosphate synthase complex with either SRT1 or RER2.1 Publication

Protein-protein interaction databases

BioGridi31852, 280 interactors
DIPiDIP-1220N
IntActiQ12063, 13 interactors
STRINGi4932.YDL193W

Structurei

3D structure databases

ProteinModelPortaliQ12063
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UPP synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003223
HOGENOMiHOG000169041
InParanoidiQ12063
KOiK19177
OMAiHIRLTEF
OrthoDBiEOG092C50UF

Family and domain databases

Gene3Di3.40.1180.10, 1 hit
InterProiView protein in InterPro
IPR036424 UPP_synth-like_sf
SUPFAMiSSF64005 SSF64005, 1 hit

Sequencei

Sequence statusi: Complete.

Q12063-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTMIKKDDK AMEPPNEKPH RKIERDDVPE SSNHIPPPES GVLKGGKVNS
60 70 80 90 100
KTRALKAVTS IIADADENPQ KKVNNETNGV QKQKTEDLSK RIGKFEYLFY
110 120 130 140 150
KFLLVLLYIC FGLFRYGQYQ YNKMKLRIFS IIYNHAYTPQ LIRQDVIPLK
160 170 180 190 200
KIPKRLAAIL EVKPVGDVGG GVTGLLNDAS EIVCWTVSAG IKHLMLYDYD
210 220 230 240 250
GILQRNVPEL RMEIHSNLAK YFGPAHVPNY AVKIPHSNKI FYNLDGIETE
260 270 280 290 300
TDVGNEIEAN QEKDKIAIEI SLLSNRDGRE TIVDLTKTMA ELCAVNELSV
310 320 330 340 350
SDITMDLVDS ELKQLVGPEP DLLLYFGPSL DLQGFPPWHI RLTEFYWEKD
360 370
NNEVIYSVFI RGLRQYAGCK VNVGK
Length:375
Mass (Da):42,557
Last modified:November 1, 1996 - v1
Checksum:iAFB4A55F242C94D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83276 Genomic DNA Translation: CAA58254.1
Z74241 Genomic DNA Translation: CAA98770.1
BK006938 Genomic DNA Translation: DAA11670.1
PIRiS58784
RefSeqiNP_010088.1, NM_001180253.1

Genome annotation databases

EnsemblFungiiYDL193W; YDL193W; YDL193W
GeneIDi851334
KEGGisce:YDL193W

Similar proteinsi

Entry informationi

Entry nameiUPPS_YEAST
AccessioniPrimary (citable) accession number: Q12063
Secondary accession number(s): D6VRG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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