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Protein

Dehydrodolichyl diphosphate synthase complex subunit NUS1

Gene

NUS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

With SRT1 or RER2, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precusrosor of dolichol which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER).1 Publication

Catalytic activityi

(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10-55).1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • dolichol biosynthetic process Source: SGD
  • protein glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-29578-MONOMER.
ReactomeiR-SCE-446199. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrodolichyl diphosphate synthase complex subunit NUS1Curated (EC:2.5.1.871 Publication)
Alternative name(s):
Di-trans,poly-cis-decaprenylcistransferaseCurated
Nuclear undecaprenyl pyrophosphate synthase 1Imported
Undecaprenyl diphosphate synthase1 Publication
Short name:
UDSCurated
Gene namesi
Name:NUS1
Ordered Locus Names:YDL193WImported
ORF Names:D12391 Publication
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL193W.
SGDiS000002352. NUS1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei97 – 11923HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • dehydrodolichyl diphosphate synthase complex Source: SGD
  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • lipid particle Source: SGD
  • nuclear membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi372 – 3721N → H: Loss of function.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Dehydrodolichyl diphosphate synthase complex subunit NUS1PRO_0000242134Add
BLAST

Proteomic databases

MaxQBiQ12063.

PTM databases

iPTMnetiQ12063.

Interactioni

Subunit structurei

Forms an active dehydrodolichyl diphosphate synthase complex with either SRT1 or RER2.1 Publication

Protein-protein interaction databases

BioGridi31852. 35 interactions.
DIPiDIP-1220N.
IntActiQ12063. 2 interactions.
MINTiMINT-479558.

Structurei

3D structure databases

ProteinModelPortaliQ12063.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UPP synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003223.
HOGENOMiHOG000169041.
InParanoidiQ12063.
KOiK19177.
OMAiATWCACA.
OrthoDBiEOG7FBRTH.

Family and domain databases

Gene3Di3.40.1180.10. 1 hit.
InterProiIPR001441. UPP_synth-like.
[Graphical view]
SUPFAMiSSF64005. SSF64005. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12063-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTMIKKDDK AMEPPNEKPH RKIERDDVPE SSNHIPPPES GVLKGGKVNS
60 70 80 90 100
KTRALKAVTS IIADADENPQ KKVNNETNGV QKQKTEDLSK RIGKFEYLFY
110 120 130 140 150
KFLLVLLYIC FGLFRYGQYQ YNKMKLRIFS IIYNHAYTPQ LIRQDVIPLK
160 170 180 190 200
KIPKRLAAIL EVKPVGDVGG GVTGLLNDAS EIVCWTVSAG IKHLMLYDYD
210 220 230 240 250
GILQRNVPEL RMEIHSNLAK YFGPAHVPNY AVKIPHSNKI FYNLDGIETE
260 270 280 290 300
TDVGNEIEAN QEKDKIAIEI SLLSNRDGRE TIVDLTKTMA ELCAVNELSV
310 320 330 340 350
SDITMDLVDS ELKQLVGPEP DLLLYFGPSL DLQGFPPWHI RLTEFYWEKD
360 370
NNEVIYSVFI RGLRQYAGCK VNVGK
Length:375
Mass (Da):42,557
Last modified:November 1, 1996 - v1
Checksum:iAFB4A55F242C94D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83276 Genomic DNA. Translation: CAA58254.1.
Z74241 Genomic DNA. Translation: CAA98770.1.
BK006938 Genomic DNA. Translation: DAA11670.1.
PIRiS58784.
RefSeqiNP_010088.1. NM_001180253.1.

Genome annotation databases

EnsemblFungiiYDL193W; YDL193W; YDL193W.
GeneIDi851334.
KEGGisce:YDL193W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83276 Genomic DNA. Translation: CAA58254.1.
Z74241 Genomic DNA. Translation: CAA98770.1.
BK006938 Genomic DNA. Translation: DAA11670.1.
PIRiS58784.
RefSeqiNP_010088.1. NM_001180253.1.

3D structure databases

ProteinModelPortaliQ12063.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31852. 35 interactions.
DIPiDIP-1220N.
IntActiQ12063. 2 interactions.
MINTiMINT-479558.

PTM databases

iPTMnetiQ12063.

Proteomic databases

MaxQBiQ12063.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL193W; YDL193W; YDL193W.
GeneIDi851334.
KEGGisce:YDL193W.

Organism-specific databases

EuPathDBiFungiDB:YDL193W.
SGDiS000002352. NUS1.

Phylogenomic databases

GeneTreeiENSGT00390000003223.
HOGENOMiHOG000169041.
InParanoidiQ12063.
KOiK19177.
OMAiATWCACA.
OrthoDBiEOG7FBRTH.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:G3O-29578-MONOMER.
ReactomeiR-SCE-446199. Synthesis of Dolichyl-phosphate.

Miscellaneous databases

PROiQ12063.

Family and domain databases

Gene3Di3.40.1180.10. 1 hit.
InterProiIPR001441. UPP_synth-like.
[Graphical view]
SUPFAMiSSF64005. SSF64005. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, found on a 12.5 kbp fragment of chromosome IV of Saccharomyces cerevisiae."
    Verhasselt P., Voet M., Volckaert G.
    Yeast 11:961-966(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae."
    Athenstaedt K., Zweytick D., Jandrositz A., Kohlwein S.D., Daum G.
    J. Bacteriol. 181:6441-6448(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "A novel strategy for constructing N-terminal chromosomal fusions to green fluorescent protein in the yeast Saccharomyces cerevisiae."
    Prein B., Natter K., Kohlwein S.D.
    FEBS Lett. 485:29-34(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. Cited for: SUBCELLULAR LOCATION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation."
    Park E.J., Grabinska K.A., Guan Z., Stranecky V., Hartmannova H., Hodanova K., Baresova V., Sovova J., Jozsef L., Ondruskova N., Hansikova H., Honzik T., Zeman J., Hulkova H., Wen R., Kmoch S., Sessa W.C.
    Cell Metab. 20:448-457(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION, SUBUNIT, MUTAGENESIS OF ASN-372.

Entry informationi

Entry nameiUPPS_YEAST
AccessioniPrimary (citable) accession number: Q12063
Secondary accession number(s): D6VRG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.