SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q12062

- AFLK_ASPPA

UniProt

Q12062 - AFLK_ASPPA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Versicolorin B synthase
Gene
vbs, aflK
Organism
Aspergillus parasiticus
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes 2 dehydration steps in the aflatoxin biosynthesis pathway: (1) acts as an 5'-oxoaverantin cyclase that mediates conversion of 5'-oxoaverantin (OAVN) to (2'S,5'S)-averufin (AVR) and (2) acts as a versicolorin B synthase by catalyzing conversion of versiconal (VHOH) to versicolorin B (VB).5 Publications

Catalytic activityi

Versiconal = versicolorin B + H2O.4 Publications
5'-oxoaverantin = (1'S,5'S)-averufin + H2O.4 Publications

Cofactori

FAD By similarity.

Kineticsi

  1. KM=20 µM for 5'-oxoaverantin2 Publications
  2. KM=3.1 µM for versiconal (at pH 6.0 and 30 degrees Celsius)

Vmax=6.67 nmol/min/mg enzyme with 5'-oxoaverantin as substrate

Vmax=0.15 µmol/min/mg enzyme with versiconal (at pH 6.0 and 30 degrees Celsius) as substrate

pH dependencei

Optimum pH is 5.5.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 10530FAD By similarity
Add
BLAST

GO - Molecular functioni

  1. choline dehydrogenase activity Source: InterPro
  2. flavin adenine dinucleotide binding Source: InterPro
  3. hydro-lyase activity Source: UniProtKB
  4. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. aflatoxin biosynthetic process Source: UniProtKB
  2. alcohol metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14040.
UniPathwayiUPA00287.

Names & Taxonomyi

Protein namesi
Recommended name:
Versicolorin B synthase (EC:4.2.1.143)
Alternative name(s):
5'-oxoaverantin cyclase (EC:4.2.1.142)
Aflatoxin biosynthesis protein K
Gene namesi
Name:vbs
Synonyms:aflK
OrganismiAspergillus parasiticus
Taxonomic identifieri5067 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

Cytoplasmcytosol Inferred 1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 4141
PRO_0000424163Add
BLAST
Chaini22 – 643622Versicolorin B synthase
PRO_0000424164Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi221 – 2211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi507 – 5071N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homodimer.4 Publications

Structurei

3D structure databases

ProteinModelPortaliQ12062.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12062-1 [UniParc]FASTAAdd to Basket

« Hide

MGRNWFQVTA MAVVPVVGIM AAVNPTILSS AASSLPSLGA MFTSDDFASQ    50
MDGRIQAQGL LSSHFGMYGW PGQSFDYVIV GGGTAGLAMA RRLSQDGTAS 100
VAVIEAGGFY ETDAGNATEV PMYLFNYFFD NGKVKNPLFD WYQYTTPQPG 150
LAQREMFYMQ GKTLGGSTAR GAMLYHRGSK GAYDMWADHV GDDSYRWDKW 200
LPYFQKSVHF SGPETNPRPA NATALNDNTA FTASGGPVHV GYPFQVNAIS 250
SWVDRALAKM GFPEAQGFSN GNLLGRSYIT HTINPYTRRR ETASSSYLRE 300
ALMESNNLNI FTRTLVKRVL FDDQNRATGV TVNTDGFEWQ IGARKEVILS 350
AGVMRSPQLL MVSGIGPKDH LEQLGIPVRS DLSGVGQNMQ DTIILGPTVP 400
VKVESHSQLM GNKETLPRAI REYNEQRKGL LTNPGQDYFA FEKHQPGMLK 450
ESTAADIDAA FPDDWPTFSY IALDDTFVPQ YDGKNYFSMS AALMTPFSRG 500
TVTINSNDTA NPPIVDPQWL ADPRDQEMAV AAFRRCREIV ASDVMREVVA 550
GPEILPGPQY QTDEEILNYI AETSDAYYAG VGTCAMGKAD DPKAVVDSKA 600
RVLGVKGLRI VDASIFPFAI DGQPMGTVYA LAEKIAAEMM AGQ 643
Length:643
Mass (Da):70,271
Last modified:November 1, 1996 - v1
Checksum:i4B903BA6A0F39D11
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51327 Genomic DNA. Translation: AAC49318.1.
U51328 mRNA. Translation: AAC49319.1.
AY371490 Genomic DNA. Translation: AAS66012.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51327 Genomic DNA. Translation: AAC49318.1 .
U51328 mRNA. Translation: AAC49319.1 .
AY371490 Genomic DNA. Translation: AAS66012.1 .

3D structure databases

ProteinModelPortali Q12062.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00287 .
BioCyci MetaCyc:MONOMER-14040.

Family and domain databases

InterProi IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view ]
Pfami PF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000137. Alcohol_oxidase. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of the versicolorin B synthase gene from Aspergillus parasiticus. Expansion of the aflatoxin b1 biosynthetic gene cluster."
    Silva J.C., Minto R.E., Barry C.E., Holland K.A., Townsend C.A.
    J. Biol. Chem. 271:13600-13608(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 163-180 AND 414-428, FUNCTION, SUBUNIT.
    Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
  2. "Completed sequence of aflatoxin pathway gene cluster in Aspergillus parasiticus."
    Yu J., Bhatnagar D., Cleveland T.E.
    FEBS Lett. 564:126-130(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
    Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
  3. "Aspergillus parasiticus cyclase catalyzes two dehydration steps in aflatoxin biosynthesis."
    Sakuno E., Wen Y., Hatabayashi H., Arai H., Aoki C., Yabe K., Nakajima H.
    Appl. Environ. Microbiol. 71:2999-3006(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 41-66, FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, SUBUNIT.
  4. "Purification and properties of versiconal cyclase from Aspergillus parasiticus."
    Lin B.K., Anderson J.A.
    Arch. Biochem. Biophys. 293:67-70(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
  5. "Purification and characterization of versicolorin B synthase from Aspergillus parasiticus. Catalysis of the stereodifferentiating cyclization in aflatoxin biosynthesis essential to DNA interaction."
    McGuire S.M., Silva J.C., Casillas E.G., Townsend C.A.
    Biochemistry 35:11470-11486(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
  6. "Heterologous expression, isolation, and characterization of versicolorin B synthase from Aspergillus parasiticus. A key enzyme in the aflatoxin B1 biosynthetic pathway."
    Silva J.C., Townsend C.A.
    J. Biol. Chem. 272:804-813(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.
    Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
  7. "Involvement of two cytosolic enzymes and a novel intermediate, 5'-oxoaverantin, in the pathway from 5'-hydroxyaverantin to averufin in aflatoxin biosynthesis."
    Sakuno E., Yabe K., Nakajima H.
    Appl. Environ. Microbiol. 69:6418-6426(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
  8. Cited for: REVIEW.
    Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.

Entry informationi

Entry nameiAFLK_ASPPA
AccessioniPrimary (citable) accession number: Q12062
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi