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Q12062

- AFLK_ASPPA

UniProt

Q12062 - AFLK_ASPPA

Protein

Versicolorin B synthase

Gene

vbs

Organism
Aspergillus parasiticus
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes 2 dehydration steps in the aflatoxin biosynthesis pathway: (1) acts as an 5'-oxoaverantin cyclase that mediates conversion of 5'-oxoaverantin (OAVN) to (2'S,5'S)-averufin (AVR) and (2) acts as a versicolorin B synthase by catalyzing conversion of versiconal (VHOH) to versicolorin B (VB).5 Publications

    Catalytic activityi

    Versiconal = versicolorin B + H2O.
    5'-oxoaverantin = (1'S,5'S)-averufin + H2O.

    Cofactori

    FAD.By similarity

    Kineticsi

    1. KM=20 µM for 5'-oxoaverantin2 Publications
    2. KM=3.1 µM for versiconal (at pH 6.0 and 30 degrees Celsius)2 Publications

    Vmax=6.67 nmol/min/mg enzyme with 5'-oxoaverantin as substrate2 Publications

    Vmax=0.15 µmol/min/mg enzyme with versiconal (at pH 6.0 and 30 degrees Celsius) as substrate2 Publications

    pH dependencei

    Optimum pH is 5.5.2 Publications

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi76 – 10530FADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. choline dehydrogenase activity Source: InterPro
    2. flavin adenine dinucleotide binding Source: InterPro
    3. hydro-lyase activity Source: UniProtKB
    4. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. aflatoxin biosynthetic process Source: UniProtKB
    2. alcohol metabolic process Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14040.
    UniPathwayiUPA00287.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Versicolorin B synthase (EC:4.2.1.143)
    Alternative name(s):
    5'-oxoaverantin cyclase (EC:4.2.1.142)
    Aflatoxin biosynthesis protein K
    Gene namesi
    Name:vbs
    Synonyms:aflK
    OrganismiAspergillus parasiticus
    Taxonomic identifieri5067 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

    Subcellular locationi

    Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 4141PRO_0000424163Add
    BLAST
    Chaini22 – 643622Versicolorin B synthasePRO_0000424164Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi221 – 2211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi507 – 5071N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer.4 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ12062.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GMC oxidoreductase family.Curated

    Family and domain databases

    InterProiIPR012132. GMC_OxRdtase.
    IPR000172. GMC_OxRdtase_N.
    IPR007867. GMC_OxRtase_C.
    [Graphical view]
    PfamiPF05199. GMC_oxred_C. 1 hit.
    PF00732. GMC_oxred_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12062-1 [UniParc]FASTAAdd to Basket

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    MGRNWFQVTA MAVVPVVGIM AAVNPTILSS AASSLPSLGA MFTSDDFASQ    50
    MDGRIQAQGL LSSHFGMYGW PGQSFDYVIV GGGTAGLAMA RRLSQDGTAS 100
    VAVIEAGGFY ETDAGNATEV PMYLFNYFFD NGKVKNPLFD WYQYTTPQPG 150
    LAQREMFYMQ GKTLGGSTAR GAMLYHRGSK GAYDMWADHV GDDSYRWDKW 200
    LPYFQKSVHF SGPETNPRPA NATALNDNTA FTASGGPVHV GYPFQVNAIS 250
    SWVDRALAKM GFPEAQGFSN GNLLGRSYIT HTINPYTRRR ETASSSYLRE 300
    ALMESNNLNI FTRTLVKRVL FDDQNRATGV TVNTDGFEWQ IGARKEVILS 350
    AGVMRSPQLL MVSGIGPKDH LEQLGIPVRS DLSGVGQNMQ DTIILGPTVP 400
    VKVESHSQLM GNKETLPRAI REYNEQRKGL LTNPGQDYFA FEKHQPGMLK 450
    ESTAADIDAA FPDDWPTFSY IALDDTFVPQ YDGKNYFSMS AALMTPFSRG 500
    TVTINSNDTA NPPIVDPQWL ADPRDQEMAV AAFRRCREIV ASDVMREVVA 550
    GPEILPGPQY QTDEEILNYI AETSDAYYAG VGTCAMGKAD DPKAVVDSKA 600
    RVLGVKGLRI VDASIFPFAI DGQPMGTVYA LAEKIAAEMM AGQ 643
    Length:643
    Mass (Da):70,271
    Last modified:November 1, 1996 - v1
    Checksum:i4B903BA6A0F39D11
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51327 Genomic DNA. Translation: AAC49318.1.
    U51328 mRNA. Translation: AAC49319.1.
    AY371490 Genomic DNA. Translation: AAS66012.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51327 Genomic DNA. Translation: AAC49318.1 .
    U51328 mRNA. Translation: AAC49319.1 .
    AY371490 Genomic DNA. Translation: AAS66012.1 .

    3D structure databases

    ProteinModelPortali Q12062.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00287 .
    BioCyci MetaCyc:MONOMER-14040.

    Family and domain databases

    InterProi IPR012132. GMC_OxRdtase.
    IPR000172. GMC_OxRdtase_N.
    IPR007867. GMC_OxRtase_C.
    [Graphical view ]
    Pfami PF05199. GMC_oxred_C. 1 hit.
    PF00732. GMC_oxred_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000137. Alcohol_oxidase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the versicolorin B synthase gene from Aspergillus parasiticus. Expansion of the aflatoxin b1 biosynthetic gene cluster."
      Silva J.C., Minto R.E., Barry C.E., Holland K.A., Townsend C.A.
      J. Biol. Chem. 271:13600-13608(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 163-180 AND 414-428, FUNCTION, SUBUNIT.
      Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
    2. "Completed sequence of aflatoxin pathway gene cluster in Aspergillus parasiticus."
      Yu J., Bhatnagar D., Cleveland T.E.
      FEBS Lett. 564:126-130(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
      Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
    3. "Aspergillus parasiticus cyclase catalyzes two dehydration steps in aflatoxin biosynthesis."
      Sakuno E., Wen Y., Hatabayashi H., Arai H., Aoki C., Yabe K., Nakajima H.
      Appl. Environ. Microbiol. 71:2999-3006(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 41-66, FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, SUBUNIT.
    4. "Purification and properties of versiconal cyclase from Aspergillus parasiticus."
      Lin B.K., Anderson J.A.
      Arch. Biochem. Biophys. 293:67-70(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
    5. "Purification and characterization of versicolorin B synthase from Aspergillus parasiticus. Catalysis of the stereodifferentiating cyclization in aflatoxin biosynthesis essential to DNA interaction."
      McGuire S.M., Silva J.C., Casillas E.G., Townsend C.A.
      Biochemistry 35:11470-11486(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
    6. "Heterologous expression, isolation, and characterization of versicolorin B synthase from Aspergillus parasiticus. A key enzyme in the aflatoxin B1 biosynthetic pathway."
      Silva J.C., Townsend C.A.
      J. Biol. Chem. 272:804-813(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION.
      Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
    7. "Involvement of two cytosolic enzymes and a novel intermediate, 5'-oxoaverantin, in the pathway from 5'-hydroxyaverantin to averufin in aflatoxin biosynthesis."
      Sakuno E., Yabe K., Nakajima H.
      Appl. Environ. Microbiol. 69:6418-6426(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    8. Cited for: REVIEW.
      Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.

    Entry informationi

    Entry nameiAFLK_ASPPA
    AccessioniPrimary (citable) accession number: Q12062
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3