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Protein

Transcriptional coactivator HFI1/ADA1

Gene

HFI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. HFI1/ADA1 and SPT20/ADA5 may recruit TATA binding protein (TBP) and possibly other basal factors to bind to the TATA box.1 Publication

GO - Molecular functioni

  • transcription coactivator activity Source: SGD
  • transcription cofactor activity Source: SGD

GO - Biological processi

  • chromatin modification Source: SGD
  • histone acetylation Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34139-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional coactivator HFI1/ADA1
Gene namesi
Name:HFI1
Synonyms:ADA1, SUP110
Ordered Locus Names:YPL254W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL254W.
SGDiS000006175. HFI1.

Subcellular locationi

GO - Cellular componenti

  • Ada2/Gcn5/Ada3 transcription activator complex Source: SGD
  • SAGA complex Source: SGD
  • SLIK (SAGA-like) complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Transcriptional coactivator HFI1/ADA1PRO_0000083959Add
BLAST

Proteomic databases

MaxQBiQ12060.
PeptideAtlasiQ12060.

PTM databases

iPTMnetiQ12060.

Interactioni

Subunit structurei

Component of the 1.8 MDa SAGA complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA. Component of the SALSA complex, which consists of at least TRA1, SPT7 (C-terminal truncated form), TAF5, ADA3, SPT20, TAF12, TAF6, HFI1, GCN5, ADA2 and SPT3. Component of the SLIK complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SPT20, RTG2, TAF12, TAF6, HFI1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10 and TAF9. Component of the ADA/GCN5 complex, that consists of HFI1/ADA1, ADA2, ADA3, SPT20/ADA5 and GCN5 and is probably a subcomplex of SAGA.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ADA2Q0233622EBI-8287,EBI-2186
GCN5Q0333020EBI-8287,EBI-7458
NGG1P324945EBI-8287,EBI-2192
SGF29P255549EBI-8287,EBI-21678
SPT20P5087510EBI-8287,EBI-17751
SPT3P068449EBI-8287,EBI-17921
SPT7P3517716EBI-8287,EBI-17958
SPT8P3891514EBI-8287,EBI-17964
TAF10Q120307EBI-8287,EBI-18889
TAF12Q0376110EBI-8287,EBI-35097
TAF5P3812915EBI-8287,EBI-18868
TAF6P530409EBI-8287,EBI-18876
TRA1P388118EBI-8287,EBI-24638
UBP8P501029EBI-8287,EBI-19863

Protein-protein interaction databases

BioGridi35909. 157 interactions.
DIPiDIP-955N.
IntActiQ12060. 99 interactions.
MINTiMINT-631666.

Structurei

3D structure databases

ProteinModelPortaliQ12060.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000111175.
InParanoidiQ12060.
KOiK11362.
OMAiNIPKIPI.
OrthoDBiEOG7ZWDBS.

Family and domain databases

InterProiIPR024738. Hfi1/Tada1.
[Graphical view]
PfamiPF12767. SAGA-Tad1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIQSPAPK PLQPTYPAAS PASTNAYMKP GLIGSPAVSN HTEPNNGNNE
60 70 80 90 100
TAEPQGPNQR IDLGAMIEEL TSLLGKESWT KYAQIISLFI LGKLSRKELS
110 120 130 140 150
NELELVFSPS AASLEKSNTN HHHSLVRLHN QLLLGIFANS LRENPLGRNG
160 170 180 190 200
NESSWGFGNG SNNPNNKLKR INKHNSQIEV YKKIVMSLPL NDRNRLKMIT
210 220 230 240 250
KEAGKRGFIF CSVFQARLNN IPKIPIVTNP ESLKRVKSNN LKTPLEWSQD
260 270 280 290 300
IMNGFNVPLA SESHSLPDTD SFYLRMVGIA REHGLVGTVD ARCVELISLA
310 320 330 340 350
LDQYLKNIIE FTIDTVRYRR KKYSDYYDLN ESGLYKSVSE MAADKRDAKI
360 370 380 390 400
KQLDDDKNED ECADEAKSIN NGNNSSKDDI GDISMSSITK AGEAVNEELH
410 420 430 440 450
ENRTISLTNE DIYDSLSIFP NLVEPSGSYY ALTNLGLVND DELVDMKSNI
460 470 480
DDLPDFLNEK PTFTPLDERN VGTRHELNWL IKGILTED
Length:488
Mass (Da):54,466
Last modified:November 1, 1996 - v1
Checksum:iB3ACF3C6101AB541
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191A → V in AAA84983 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76735 Genomic DNA. Translation: AAB58359.1.
U41324 Genomic DNA. Translation: AAA84983.1.
Z67751 Genomic DNA. Translation: CAA91590.1.
Z73610 Genomic DNA. Translation: CAA97979.1.
BK006949 Genomic DNA. Translation: DAA11183.1.
PIRiS61010.
RefSeqiNP_015069.1. NM_001184068.1.

Genome annotation databases

EnsemblFungiiYPL254W; YPL254W; YPL254W.
GeneIDi855821.
KEGGisce:YPL254W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76735 Genomic DNA. Translation: AAB58359.1.
U41324 Genomic DNA. Translation: AAA84983.1.
Z67751 Genomic DNA. Translation: CAA91590.1.
Z73610 Genomic DNA. Translation: CAA97979.1.
BK006949 Genomic DNA. Translation: DAA11183.1.
PIRiS61010.
RefSeqiNP_015069.1. NM_001184068.1.

3D structure databases

ProteinModelPortaliQ12060.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35909. 157 interactions.
DIPiDIP-955N.
IntActiQ12060. 99 interactions.
MINTiMINT-631666.

PTM databases

iPTMnetiQ12060.

Proteomic databases

MaxQBiQ12060.
PeptideAtlasiQ12060.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL254W; YPL254W; YPL254W.
GeneIDi855821.
KEGGisce:YPL254W.

Organism-specific databases

EuPathDBiFungiDB:YPL254W.
SGDiS000006175. HFI1.

Phylogenomic databases

HOGENOMiHOG000111175.
InParanoidiQ12060.
KOiK11362.
OMAiNIPKIPI.
OrthoDBiEOG7ZWDBS.

Enzyme and pathway databases

BioCyciYEAST:G3O-34139-MONOMER.

Miscellaneous databases

PROiQ12060.

Family and domain databases

InterProiIPR024738. Hfi1/Tada1.
[Graphical view]
PfamiPF12767. SAGA-Tad1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ADA1, a novel component of the ADA/GCN5 complex, has broader effects than GCN5, ADA2, or ADA3."
    Horiuchi J., Silverman N., Pina B., Marcus G.A., Guarente L.
    Mol. Cell. Biol. 17:3220-3228(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN THE ADA/GCN5 COMPLEX.
    Strain: ATCC MYA-3516 / BWG1-7A.
  2. Brown N.G.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation."
    Grant P.A., Schieltz D., Pray-Grant M.G., Steger D.J., Reese J.C., Yates J.R. III, Workman J.L.
    Cell 94:45-53(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SAGA COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "The ATM-related cofactor Tra1 is a component of the purified SAGA complex."
    Grant P.A., Schieltz D., Pray-Grant M.G., Yates J.R. III, Workman J.L.
    Mol. Cell 2:863-867(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A SAGA COMPLEX WITH SPT2; SPT7; SPT8; GCN5; SPT20; ADA2; ADA3 AND TRA1.
  7. "Expanded lysine acetylation specificity of Gcn5 in native complexes."
    Grant P.A., Eberharter A., John S., Cook R.G., Turner B.M., Workman J.L.
    J. Biol. Chem. 274:5895-5900(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HISTONE ACETYLATION AT THE SAGA COMPLEX.
  8. "The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway."
    Pray-Grant M.G., Schieltz D., McMahon S.J., Wood J.M., Kennedy E.L., Cook R.G., Workman J.L., Yates J.R. III, Grant P.A.
    Mol. Cell. Biol. 22:8774-8786(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SLIK COMPLEX.
  9. "SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription."
    Sterner D.E., Belotserkovskaya R., Berger S.L.
    Proc. Natl. Acad. Sci. U.S.A. 99:11622-11627(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SALSA COMPLEX.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation."
    Pray-Grant M.G., Daniel J.A., Schieltz D., Yates J.R. III, Grant P.A.
    Nature 433:434-438(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SLIK COMPLEX.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Molecular architecture of the S. cerevisiae SAGA complex."
    Wu P.Y., Ruhlmann C., Winston F., Schultz P.
    Mol. Cell 15:199-208(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF THE SAGA COMPLEX.

Entry informationi

Entry nameiHFI1_YEAST
AccessioniPrimary (citable) accession number: Q12060
Secondary accession number(s): D6W3B7, O00039, Q02813
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7950 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.