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Protein

Transcriptional coactivator HFI1/ADA1

Gene

HFI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. HFI1/ADA1 and SPT20/ADA5 may recruit TATA binding protein (TBP) and possibly other basal factors to bind to the TATA box.1 Publication

Miscellaneous

Present with 7950 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • transcription coactivator activity Source: SGD
  • transcription cofactor activity Source: SGD

GO - Biological processi

  • chromatin organization Source: SGD
  • histone acetylation Source: SGD
  • histone H3 acetylation Source: GO_Central
  • regulation of transcription by RNA polymerase II Source: GO_Central
  • transcription by RNA polymerase II Source: SGD

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34139-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional coactivator HFI1/ADA1
Gene namesi
Name:HFI1
Synonyms:ADA1, SUP110
Ordered Locus Names:YPL254W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL254W
SGDiS000006175 HFI1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000839591 – 488Transcriptional coactivator HFI1/ADA1Add BLAST488

Proteomic databases

MaxQBiQ12060
PaxDbiQ12060
PRIDEiQ12060

PTM databases

iPTMnetiQ12060

Interactioni

Subunit structurei

Component of the 1.8 MDa SAGA complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA. Component of the SALSA complex, which consists of at least TRA1, SPT7 (C-terminal truncated form), TAF5, ADA3, SPT20, TAF12, TAF6, HFI1, GCN5, ADA2 and SPT3. Component of the SLIK complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SPT20, RTG2, TAF12, TAF6, HFI1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10 and TAF9. Component of the ADA/GCN5 complex, that consists of HFI1/ADA1, ADA2, ADA3, SPT20/ADA5 and GCN5 and is probably a subcomplex of SAGA.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35909201 interactors.
DIPiDIP-955N
IntActiQ12060 105 interactors.
MINTiQ12060
STRINGi4932.YPL254W

Structurei

3D structure databases

ProteinModelPortaliQ12060
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000111175
InParanoidiQ12060
KOiK11362
OMAiYKKIVMS
OrthoDBiEOG092C3XEQ

Family and domain databases

InterProiView protein in InterPro
IPR024738 Hfi1/Tada1
PANTHERiPTHR21277 PTHR21277, 1 hit
PfamiView protein in Pfam
PF12767 SAGA-Tad1, 1 hit

Sequencei

Sequence statusi: Complete.

Q12060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIQSPAPK PLQPTYPAAS PASTNAYMKP GLIGSPAVSN HTEPNNGNNE
60 70 80 90 100
TAEPQGPNQR IDLGAMIEEL TSLLGKESWT KYAQIISLFI LGKLSRKELS
110 120 130 140 150
NELELVFSPS AASLEKSNTN HHHSLVRLHN QLLLGIFANS LRENPLGRNG
160 170 180 190 200
NESSWGFGNG SNNPNNKLKR INKHNSQIEV YKKIVMSLPL NDRNRLKMIT
210 220 230 240 250
KEAGKRGFIF CSVFQARLNN IPKIPIVTNP ESLKRVKSNN LKTPLEWSQD
260 270 280 290 300
IMNGFNVPLA SESHSLPDTD SFYLRMVGIA REHGLVGTVD ARCVELISLA
310 320 330 340 350
LDQYLKNIIE FTIDTVRYRR KKYSDYYDLN ESGLYKSVSE MAADKRDAKI
360 370 380 390 400
KQLDDDKNED ECADEAKSIN NGNNSSKDDI GDISMSSITK AGEAVNEELH
410 420 430 440 450
ENRTISLTNE DIYDSLSIFP NLVEPSGSYY ALTNLGLVND DELVDMKSNI
460 470 480
DDLPDFLNEK PTFTPLDERN VGTRHELNWL IKGILTED
Length:488
Mass (Da):54,466
Last modified:November 1, 1996 - v1
Checksum:iB3ACF3C6101AB541
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19A → V in AAA84983 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76735 Genomic DNA Translation: AAB58359.1
U41324 Genomic DNA Translation: AAA84983.1
Z67751 Genomic DNA Translation: CAA91590.1
Z73610 Genomic DNA Translation: CAA97979.1
BK006949 Genomic DNA Translation: DAA11183.1
PIRiS61010
RefSeqiNP_015069.1, NM_001184068.1

Genome annotation databases

EnsemblFungiiYPL254W; YPL254W; YPL254W
GeneIDi855821
KEGGisce:YPL254W

Similar proteinsi

Entry informationi

Entry nameiHFI1_YEAST
AccessioniPrimary (citable) accession number: Q12060
Secondary accession number(s): D6W3B7, O00039, Q02813
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome