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Protein

Adenylate kinase isoenzyme 6 homolog FAP7

Gene

FAP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity (By similarity). Involved in 18S rRNA maturation. Required for cleavage of the 20S pre-rRNA at site D in the cytoplasm. Involved in oxidative stress response. Required for POS9-dependent target gene transcription upon oxidative stress.UniRule annotation3 Publications

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44AMPUniRule annotation1
Binding sitei84ATPUniRule annotation1
Binding sitei110ATPUniRule annotation1
Binding sitei114ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 22ATPUniRule annotation6

GO - Molecular functioni

  • adenylate kinase activity Source: GO_Central
  • ATPase activity Source: UniProtKB-HAMAP
  • ATP binding Source: GO_Central
  • nucleoside-triphosphatase activity Source: SGD

GO - Biological processi

  • cellular response to oxidative stress Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29558-MONOMER.
ReactomeiR-SCE-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 6 homolog FAP7UniRule annotation (EC:2.7.4.3UniRule annotation)
Short name:
AK6UniRule annotation
Alternative name(s):
Dual activity adenylate kinase/ATPaseUniRule annotation
Short name:
AK/ATPaseUniRule annotation
POS9-activating factor 7
Gene namesi
Name:FAP7
Ordered Locus Names:YDL166C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL166C.
SGDiS000002325. FAP7.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytoplasmic stress granule Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19G → S: Sensitive to hydrogen peroxide. 1 Publication1
Mutagenesisi20K → R: Decreases the processing of 20S rRNA. 1 Publication1
Mutagenesisi21S → G: Sensitive to hydrogen peroxide. 1 Publication1
Mutagenesisi82D → A: Decreases the processing of 20S rRNA; when associated with A-84. 1 Publication1
Mutagenesisi84H → A: Decreases the processing of 20S rRNA; when associated with A-82. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539021 – 197Adenylate kinase isoenzyme 6 homolog FAP7Add BLAST197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei183PhosphotyrosineCombined sources1
Modified residuei188PhosphoserineCombined sources1
Modified residuei196PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12055.
PRIDEiQ12055.

PTM databases

iPTMnetiQ12055.

Interactioni

Subunit structurei

Interacts with RPS14B. Not a structural component of 43S pre-ribosomes, but transiently interacts with them with them by binding to RPS14.1 Publication

Protein-protein interaction databases

BioGridi31899. 44 interactors.
DIPiDIP-4793N.
IntActiQ12055. 22 interactors.
MINTiMINT-501278.

Structurei

3D structure databases

ProteinModelPortaliQ12055.
SMRiQ12055.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 61NMPbindUniRule annotationAdd BLAST24
Regioni113 – 123LIDUniRule annotationAdd BLAST11

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi28 – 35Nuclear localization signalSequence analysis8

Sequence similaritiesi

Belongs to the adenylate kinase family. AK6 subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000015930.
HOGENOMiHOG000224472.
InParanoidiQ12055.
KOiK18532.
OMAiQSWIVDE.
OrthoDBiEOG092C5GXJ.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00039. Adenylate_kinase_AK6. 1 hit.
InterProiIPR020618. Adenyl_kinase_AK6.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12055-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEARRYGPNI IVTGTPGCGK SSTCEFLKNK LKDYKYYNIS DFAKDNDCFE
60 70 80 90 100
GYDEGRKSHI VDEDKLLDML EPLLRQGNSI VDWHVNDVFP ERLIDLVVVL
110 120 130 140 150
RCDNSNLYSR LHARGYHDSK IEENLDAEIM GVVKQDAVES YEPHIVVELQ
160 170 180 190
SDTKEDMVSN VSRIVAWEKM WLEQHPDGVT NEYQGPRSDD EDDEDSE
Length:197
Mass (Da):22,723
Last modified:November 1, 1996 - v1
Checksum:i836A25B0D7E2633C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80I → V in AAS56894 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67750 Genomic DNA. Translation: CAA91580.1.
Z74214 Genomic DNA. Translation: CAA98740.1.
AY558568 Genomic DNA. Translation: AAS56894.1.
BK006938 Genomic DNA. Translation: DAA11695.1.
PIRiS61047.
RefSeqiNP_010115.1. NM_001180226.1.

Genome annotation databases

EnsemblFungiiYDL166C; YDL166C; YDL166C.
GeneIDi851388.
KEGGisce:YDL166C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67750 Genomic DNA. Translation: CAA91580.1.
Z74214 Genomic DNA. Translation: CAA98740.1.
AY558568 Genomic DNA. Translation: AAS56894.1.
BK006938 Genomic DNA. Translation: DAA11695.1.
PIRiS61047.
RefSeqiNP_010115.1. NM_001180226.1.

3D structure databases

ProteinModelPortaliQ12055.
SMRiQ12055.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31899. 44 interactors.
DIPiDIP-4793N.
IntActiQ12055. 22 interactors.
MINTiMINT-501278.

PTM databases

iPTMnetiQ12055.

Proteomic databases

MaxQBiQ12055.
PRIDEiQ12055.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL166C; YDL166C; YDL166C.
GeneIDi851388.
KEGGisce:YDL166C.

Organism-specific databases

EuPathDBiFungiDB:YDL166C.
SGDiS000002325. FAP7.

Phylogenomic databases

GeneTreeiENSGT00390000015930.
HOGENOMiHOG000224472.
InParanoidiQ12055.
KOiK18532.
OMAiQSWIVDE.
OrthoDBiEOG092C5GXJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-29558-MONOMER.
ReactomeiR-SCE-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

PROiQ12055.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00039. Adenylate_kinase_AK6. 1 hit.
InterProiIPR020618. Adenyl_kinase_AK6.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKAD6_YEAST
AccessioniPrimary (citable) accession number: Q12055
Secondary accession number(s): D6VRI5, E9P8W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8350 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.