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Protein

Trimethylguanosine synthase

Gene

TGS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the two serial methylation steps for the conversion of the 7-monomethylguanosine (m7G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylates the m3G cap on TLC1 telomerase which affects telomere silencing and telomere length regulation. Required for pre-mRNA splicing, pre-rRNA processing and small ribosomal subunit synthesis. Involved in nucleolar structural organization.5 Publications

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,7)G(5')pppR-RNA.
S-adenosyl-L-methionine + m(2,7)G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,2,7)G(5')pppR-RNA.

Enzyme regulationi

Substrate inhibited by S-adenosyl-L-homocysteine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei126 – 1261S-adenosyl-L-methionineCurated

GO - Molecular functioni

  • RNA methyltransferase activity Source: SGD

GO - Biological processi

  • 7-methylguanosine cap hypermethylation Source: SGD
  • 7-methylguanosine RNA capping Source: InterPro
  • meiotic nuclear division Source: SGD
  • nucleologenesis Source: SGD
  • regulation of telomere maintenance via telomerase Source: SGD
  • RNA methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-34053-MONOMER.
BRENDAi2.3.1.221. 530.

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylguanosine synthase (EC:2.1.1.-)
Alternative name(s):
Cap-specific guanine-N2 methyltransferase
snRNA/snoRNA cap hypermethylase
Gene namesi
Name:TGS1
Ordered Locus Names:YPL157W
ORF Names:P2573
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL157W.
SGDiS000006078. TGS1.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi75 – 751W → A: No effect. 1 Publication
Mutagenesisi81 – 811E → A: No effect. 1 Publication
Mutagenesisi83 – 831I → R: Loss of function. 1 Publication
Mutagenesisi103 – 1031D → A: Loss of catalytic activity, but not required for pre-rRNA processing. 2 Publications
Mutagenesisi126 – 1261D → A: Loss of function. 1 Publication
Mutagenesisi175 – 1751S → A: No effect. 1 Publication
Mutagenesisi175 – 1751S → E or R: Loss of function. 1 Publication
Mutagenesisi178 – 1781W → A: Loss of catalytic activity, but not required for pre-rRNA processing. 3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Trimethylguanosine synthasePRO_0000270619Add
BLAST

Proteomic databases

MaxQBiQ12052.

PTM databases

iPTMnetiQ12052.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with the spliceosomal snRNP core component SMB1 and the snoRNP components CBF5 and NOP58.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SWM2P403425EBI-30910,EBI-28438

Protein-protein interaction databases

BioGridi36026. 197 interactions.
DIPiDIP-1974N.
IntActiQ12052. 4 interactions.
MINTiMINT-406225.

Structurei

3D structure databases

ProteinModelPortaliQ12052.
SMRiQ12052. Positions 48-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 5858Required for correct nucleolar localizationAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000018056.
HOGENOMiHOG000216181.
InParanoidiQ12052.
KOiK14292.
OMAiAKCKVLY.
OrthoDBiEOG7GJ6PQ.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019012. RNA_cap_Gua-N2-MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF09445. Methyltransf_15. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRTFIHASK IKHAARKRKH HSNFRTLIKL LNNDAYKIES SKPLKNGKLF
60 70 80 90 100
KYWKNRRRLF SKIDSASIYM TDELWFSVTP ERIACFLANF VKACMPNAER
110 120 130 140 150
ILDVFCGGGG NTIQFAMQFP YVYGVDYSIE HIYCTAKNAQ SYGVDDRIWL
160 170 180 190 200
KRGSWKKLVS KQKLSKIKYD CVFGSPPWGG PEYLRNDVYD LEQHLKPMGI
210 220 230 240 250
TKMLKSFLKL SPNVIMFLPR NSDLNQLSRA TRKVLGPFAK CKVLYVKENG
260 270 280 290 300
YMKGIFCMWG ECFFNYEPAS TENSRRESSE KEELSSENEE LSKRKKHEST
310
TTTKDNTVDI YDVNG
Length:315
Mass (Da):36,527
Last modified:November 1, 1996 - v1
Checksum:iA7E0ABBE915D6CD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65564.1.
Z73513 Genomic DNA. Translation: CAA97862.1.
BK006949 Genomic DNA. Translation: DAA11277.1.
PIRiS65168.
RefSeqiNP_015168.1. NM_001183971.1.

Genome annotation databases

EnsemblFungiiYPL157W; YPL157W; YPL157W.
GeneIDi855946.
KEGGisce:YPL157W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65564.1.
Z73513 Genomic DNA. Translation: CAA97862.1.
BK006949 Genomic DNA. Translation: DAA11277.1.
PIRiS65168.
RefSeqiNP_015168.1. NM_001183971.1.

3D structure databases

ProteinModelPortaliQ12052.
SMRiQ12052. Positions 48-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36026. 197 interactions.
DIPiDIP-1974N.
IntActiQ12052. 4 interactions.
MINTiMINT-406225.

PTM databases

iPTMnetiQ12052.

Proteomic databases

MaxQBiQ12052.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL157W; YPL157W; YPL157W.
GeneIDi855946.
KEGGisce:YPL157W.

Organism-specific databases

EuPathDBiFungiDB:YPL157W.
SGDiS000006078. TGS1.

Phylogenomic databases

GeneTreeiENSGT00390000018056.
HOGENOMiHOG000216181.
InParanoidiQ12052.
KOiK14292.
OMAiAKCKVLY.
OrthoDBiEOG7GJ6PQ.

Enzyme and pathway databases

BioCyciYEAST:G3O-34053-MONOMER.
BRENDAi2.3.1.221. 530.

Miscellaneous databases

PROiQ12052.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019012. RNA_cap_Gua-N2-MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF09445. Methyltransf_15. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
    Purnelle B., Coster F., Goffeau A.
    Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Hypermethylation of the cap structure of both yeast snRNAs and snoRNAs requires a conserved methyltransferase that is localized to the nucleolus."
    Mouaikel J., Verheggen C., Bertrand E., Tazi J., Bordonne R.
    Mol. Cell 9:891-901(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CBF5; NOP58 AND SMB1, MUTAGENESIS OF ASP-103 AND ASP-126.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "Sequence-structure-function relationships of Tgs1, the yeast snRNA/snoRNA cap hypermethylase."
    Mouaikel J., Bujnicki J.M., Tazi J., Bordonne R.
    Nucleic Acids Res. 31:4899-4909(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TRP-75; GLU-81; ILE-83; SER-175 AND TRP-178.
  7. "The small nucle(ol)ar RNA cap trimethyltransferase is required for ribosome synthesis and intact nucleolar morphology."
    Colau G., Thiry M., Leduc V., Bordonne R., Lafontaine D.L.J.
    Mol. Cell. Biol. 24:7976-7986(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-103 AND TRP-178.
  8. "Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways."
    Hausmann S., Zheng S., Costanzo M., Brost R.L., Garcin D., Boone C., Shuman S., Schwer B.
    J. Biol. Chem. 283:31706-31718(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Hypermethylation of yeast telomerase RNA by the snRNA and snoRNA methyltransferase Tgs1."
    Franke J., Gehlen J., Ehrenhofer-Murray A.E.
    J. Cell Sci. 121:3553-3560(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TRP-178.

Entry informationi

Entry nameiTGS1_YEAST
AccessioniPrimary (citable) accession number: Q12052
Secondary accession number(s): D6W3L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.