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Protein

Trimethylguanosine synthase

Gene

TGS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the two serial methylation steps for the conversion of the 7-monomethylguanosine (m7G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylates the m3G cap on TLC1 telomerase which affects telomere silencing and telomere length regulation. Required for pre-mRNA splicing, pre-rRNA processing and small ribosomal subunit synthesis. Involved in nucleolar structural organization.5 Publications

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,7)G(5')pppR-RNA.
S-adenosyl-L-methionine + m(2,7)G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,2,7)G(5')pppR-RNA.

Enzyme regulationi

Substrate inhibited by S-adenosyl-L-homocysteine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126S-adenosyl-L-methionineCurated1

GO - Molecular functioni

  • RNA methyltransferase activity Source: SGD
  • RNA trimethylguanosine synthase activity Source: GO_Central

GO - Biological processi

  • 7-methylguanosine cap hypermethylation Source: SGD
  • 7-methylguanosine RNA capping Source: InterPro
  • meiotic cell cycle Source: SGD
  • nucleologenesis Source: SGD
  • regulation of telomere maintenance via telomerase Source: SGD
  • RNA methylation Source: SGD
  • tRNA processing Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-34053-MONOMER
BRENDAi2.3.1.221 530

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylguanosine synthase (EC:2.1.1.-)
Alternative name(s):
Cap-specific guanine-N2 methyltransferase
snRNA/snoRNA cap hypermethylase
Gene namesi
Name:TGS1
Ordered Locus Names:YPL157W
ORF Names:P2573
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL157W
SGDiS000006078 TGS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75W → A: No effect. 1 Publication1
Mutagenesisi81E → A: No effect. 1 Publication1
Mutagenesisi83I → R: Loss of function. 1 Publication1
Mutagenesisi103D → A: Loss of catalytic activity, but not required for pre-rRNA processing. 2 Publications1
Mutagenesisi126D → A: Loss of function. 1 Publication1
Mutagenesisi175S → A: No effect. 1 Publication1
Mutagenesisi175S → E or R: Loss of function. 1 Publication1
Mutagenesisi178W → A: Loss of catalytic activity, but not required for pre-rRNA processing. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002706191 – 315Trimethylguanosine synthaseAdd BLAST315

Proteomic databases

MaxQBiQ12052
PaxDbiQ12052
PRIDEiQ12052

PTM databases

iPTMnetiQ12052

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with the spliceosomal snRNP core component SMB1 and the snoRNP components CBF5 and NOP58.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SWM2P403425EBI-30910,EBI-28438

Protein-protein interaction databases

BioGridi36026, 470 interactors
DIPiDIP-1974N
IntActiQ12052, 4 interactors
MINTiQ12052
STRINGi4932.YPL157W

Structurei

3D structure databases

ProteinModelPortaliQ12052
SMRiQ12052
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 58Required for correct nucleolar localizationAdd BLAST58

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000018056
HOGENOMiHOG000216181
InParanoidiQ12052
KOiK14292
OMAiAKCKVLY
OrthoDBiEOG092C4SEY

Family and domain databases

InterProiView protein in InterPro
IPR019012 RNA_cap_Gua-N2-MeTrfase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF09445 Methyltransf_15, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Q12052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRTFIHASK IKHAARKRKH HSNFRTLIKL LNNDAYKIES SKPLKNGKLF
60 70 80 90 100
KYWKNRRRLF SKIDSASIYM TDELWFSVTP ERIACFLANF VKACMPNAER
110 120 130 140 150
ILDVFCGGGG NTIQFAMQFP YVYGVDYSIE HIYCTAKNAQ SYGVDDRIWL
160 170 180 190 200
KRGSWKKLVS KQKLSKIKYD CVFGSPPWGG PEYLRNDVYD LEQHLKPMGI
210 220 230 240 250
TKMLKSFLKL SPNVIMFLPR NSDLNQLSRA TRKVLGPFAK CKVLYVKENG
260 270 280 290 300
YMKGIFCMWG ECFFNYEPAS TENSRRESSE KEELSSENEE LSKRKKHEST
310
TTTKDNTVDI YDVNG
Length:315
Mass (Da):36,527
Last modified:November 1, 1996 - v1
Checksum:iA7E0ABBE915D6CD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA Translation: CAA65564.1
Z73513 Genomic DNA Translation: CAA97862.1
BK006949 Genomic DNA Translation: DAA11277.1
PIRiS65168
RefSeqiNP_015168.1, NM_001183971.1

Genome annotation databases

EnsemblFungiiYPL157W; YPL157W; YPL157W
GeneIDi855946
KEGGisce:YPL157W

Similar proteinsi

Entry informationi

Entry nameiTGS1_YEAST
AccessioniPrimary (citable) accession number: Q12052
Secondary accession number(s): D6W3L1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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