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Reviewed, UniProtKB/Swiss-Prot Q12051 (GGPPS_YEAST)

Last modified November 25, 2008. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Geranylgeranyl pyrophosphate synthetase
      Short name=GGPP synthetase
      Short name=GGPPSase
Alternative name(s):
    Geranylgeranyl diphosphate synthase
    BET2 suppressor protein 1
Including the following 3 domains:
    1- Recommended name:
            Dimethylallyltranstransferase
              EC=2.5.1.1
    2- Recommended name:
            Geranyltranstransferase
              EC=2.5.1.10
    3- Recommended name:
            Farnesyltranstransferase
              EC=2.5.1.29
Gene names
Name: BTS1
Ordered Locus Names: YPL069C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the trans-addition of the 3 molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate. Required for the membrane attachment of YPT1 and SEC4. May be involved in vesicle trafficking and protein sorting.

Catalytic activity

Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate.

Trans,trans-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.

Pathway

Isoprenoid biosynthesis; farnesyl-PP biosynthesis; farnesyl-PP from geranyl-PP and isopentenyl-PP: step 1/1.

Isoprenoid biosynthesis; geranyl-PP biosynthesis; geranyl-PP from dimethylallyl-PP and isopentenyl-PP: step 1/1.

Isoprenoid biosynthesis; geranylgeranyl-PP biosynthesis; geranylgeranyl-PP from farnesyl-PP and isopentenyl-PP: step 1/1.

Subcellular location

CytoplasmBy similarity.

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the FPP/GGPP synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Geranylgeranyl pyrophosphate synthetase
PRO_0000228139

Sites

Active site1691 By similarity

Secondary structure

...................................... 335
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q12051-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4C7D6527FF29F157

FASTA33538,651
        10         20         30         40         50         60 
MEAKIDELIN NDPVWSSQNE SLISKPYNHI LLKPGKNFRL NLIVQINRVM NLPKDQLAIV 

        70         80         90        100        110        120 
SQIVELLHNS SLLIDDIEDN APLRRGQTTS HLIFGVPSTI NTANYMYFRA MQLVSQLTTK 

       130        140        150        160        170        180 
EPLYHNLITI FNEELINLHR GQGLDIYWRD FLPEIIPTQE MYLNMVMNKT GGLFRLTLRL 

       190        200        210        220        230        240 
MEALSPSSHH GHSLVPFINL LGIIYQIRDD YLNLKDFQMS SEKGFAEDIT EGKLSFPIVH 

       250        260        270        280        290        300 
ALNFTKTKGQ TEQHNEILRI LLLRTSDKDI KLKLIQILEF DTNSLAYTKN FINQLVNMIK 

       310        320        330 
NDNENKYLPD LASHSDTATN LHDELLYIID HLSEL 

« Hide

References

« Hide 'large scale' references
[1]"BTS1 encodes a geranylgeranyl diphosphate synthase in Saccharomyces cerevisiae."
Jiang Y., Proteau P., Poulter D., Ferro-Novick S.
J. Biol. Chem. 270:21793-21799(1995) [PubMed: 7665600] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed: 9169875] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Bph1p, the Saccharomyces cerevisiae homologue of CHS1/beige, functions in cell wall formation and protein sorting."
Shiflett S.L., Vaughn M.B., Huynh D., Kaplan J., Ward D.M.
Traffic 5:700-710(2004) [PubMed: 15296494] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

U39205 Genomic DNA. Translation: AAB68296.1.
U31632 Genomic DNA. Translation: AAA83262.1.
AY692852 Genomic DNA. Translation: AAT92871.1.
PIRS60921.
RefSeqNP_015256.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2DH4X-ray1.98A/B1-335[»]
2E8TX-ray2.13A/B1-335[»]
2E8UX-ray2.08A/B1-335[»]
2E8VX-ray1.80A/B1-335[»]
2E8WX-ray2.35A/B1-335[»]
2E8XX-ray2.04A/B1-335[»]
2E90X-ray2.55A/B1-335[»]
2E91X-ray2.14A/B1-335[»]
2E92X-ray2.31A/B1-335[»]
2E93X-ray2.12A/B1-335[»]
2E94X-ray2.18A/B1-335[»]
2E95X-ray2.20A/B1-335[»]
2Z4VX-ray1.86A/B1-335[»]
2Z4WX-ray2.45A/B1-335[»]
2Z4XX-ray1.90A/B1-335[»]
2Z4YX-ray2.10A/B1-335[»]
2Z4ZX-ray2.09A/B1-335[»]
2Z50X-ray2.01A/B1-335[»]
2Z52X-ray2.13A/B1-335[»]
2Z78X-ray2.10A/B1-335[»]
2Z7HX-ray2.08A/B1-335[»]
2Z7IX-ray2.10A/B1-335[»]
ModBaseSearch...

Protein-protein interaction databases

IntActQ12051.

Genome annotation databases

EnsemblYPL069C. Saccharomyces cerevisiae. [Contig view]
GeneID856036.
GenomeReviewsGene locus YPL069C in contig U00094_GR.
KEGGsce:YPL069C.
NMPDRfig|4932.3.peg.6389.

Organism-specific databases

CYGDYPL069c.
SGDS000005990. BTS1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMQ12051.

Enzyme and pathway databases

BioCycMetaCyc:MON-13701.

Gene expression databases

GermOnlineYPL069C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
IPR008949. Terpenoid_synth.
[Graphical view]
Gene3DG3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHERPTHR12001. Polyprenyl_synt. 1 hit.
PfamPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
PROSITEPS00723. POLYPRENYL_SYNTHET_1. 1 hit.
PS00444. POLYPRENYL_SYNTHET_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubQ12051.
NextBio980968.

Entry information

Entry nameGGPPS_YEAST
AccessionPrimary (citable) accession number: Q12051
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents