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Q12046 (CWC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor CWC2
Alternative name(s):
Complexed with CEF1 protein 2
PRP19-associated complex protein 40
Synthetic lethal with CLF1 protein 3
Gene names
Name:CWC2
Synonyms:NTC40, SLC3
Ordered Locus Names:YDL209C
ORF Names:D1041
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome. Ref.11 Ref.13

Subunit structure

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with ISY1 and PRP19. Ref.5 Ref.6 Ref.7

Subcellular location

Nucleus Ref.9.

Domain

The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding.

Miscellaneous

Present with 2650 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RRM CWC2 family.

Contains 1 C3H1-type zinc finger.

Contains 1 RRM (RNA recognition motif) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRP19P325239EBI-553,EBI-493

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 339339Pre-mRNA-splicing factor CWC2
PRO_0000081552

Regions

Domain135 – 22894RRM
Zinc finger67 – 9428C3H1-type

Amino acid modifications

Modified residue3351Phosphoserine Ref.12
Modified residue3361Phosphoserine Ref.12

Experimental info

Mutagenesis731C → Y: Inhibits cell growth. Ref.11
Mutagenesis791G → D: No effect. Synthetic lethal when associated with CLF1 lacking a TPR domain. Ref.8
Mutagenesis871C → H: Inhibits cell growth. Ref.11
Mutagenesis1861F → D: Inhibits cell growth. Ref.11

Secondary structure

................................................. 339
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q12046 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 371734A11DFDB339

FASTA33938,431
        10         20         30         40         50         60 
MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP FALQPQLHSG 

        70         80         90        100        110        120 
KTRGDNDGQL FFCLFFAKGM CCLGPKCEYL HHIPDEEDIG KLALRTEVLD CFGREKFADY 

       130        140        150        160        170        180 
REDMGGIGSF RKKNKTLYVG GIDGALNSKH LKPAQIESRI RFVFSRLGDI DRIRYVESKN 

       190        200        210        220        230        240 
CGFVKFKYQA NAEFAKEAMS NQTLLLPSDK EWDDRREGTG LLVKWANEDP DPAAQKRLQE 

       250        260        270        280        290        300 
ELKLESLNMM VHLINNNTNS AGTEVNNKNN ERLDRTFPEA SVDNVKKRLL PLDNGMESDD 

       310        320        330 
FIEKLKKVKK NISRENISSK PSVGKLGGPL LDYLSSDED 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA."
Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U., Schmidt E.R.
Yeast 13:163-169(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p."
Chen H.-R., Jan S.-P., Tsao T.Y., Sheu Y.-J., Banroques J., Cheng S.-C.
Mol. Cell. Biol. 18:2196-2204(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PRP19.
[6]"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"Characterization of interactions among the Cef1p-Prp19p-associated splicing complex."
Ohi M.D., Gould K.L.
RNA 8:798-815(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PRP19 AND ISY1.
[8]"Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing."
Vincent K., Wang Q., Jay S., Hobbs K., Rymond B.C.
Genetics 164:895-907(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF GLY-79.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing."
McGrail J.C., Krause A., O'Keefe R.T.
Nucleic Acids Res. 37:4205-4217(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF CYS-73; CYS-87 AND PHE-186, RNA-BINDING.
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335 AND SER-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre."
Rasche N., Dybkov O., Schmitzova J., Akyildiz B., Fabrizio P., Luhrmann R.
EMBO J. 31:1591-1604(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, RNA-BINDING, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99000 Genomic DNA. Translation: CAA67482.1.
Z74257 Genomic DNA. Translation: CAA98787.1.
AY693022 Genomic DNA. Translation: AAT93041.1.
BK006938 Genomic DNA. Translation: DAA11655.1.
PIRS67768.
RefSeqNP_010072.1. NM_001180269.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3TP2X-ray2.40A/B1-227[»]
3U1LX-ray1.64A1-240[»]
3U1MX-ray1.95A1-240[»]
ProteinModelPortalQ12046.
SMRQ12046. Positions 3-227.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31837. 59 interactions.
DIPDIP-5164N.
IntActQ12046. 50 interactions.
MINTMINT-510131.
STRING4932.YDL209C.

Proteomic databases

PaxDbQ12046.
PeptideAtlasQ12046.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL209C; YDL209C; YDL209C.
GeneID851318.
KEGGsce:YDL209C.

Organism-specific databases

CYGDYDL209c.
SGDS000002368. CWC2.

Phylogenomic databases

eggNOGNOG288665.
HOGENOMHOG000172735.
OMARIRYVES.
OrthoDBEOG7D867D.

Enzyme and pathway databases

BioCycYEAST:G3O-29591-MONOMER.

Gene expression databases

GenevestigatorQ12046.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR000571. Znf_CCCH.
[Graphical view]
PfamPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968354.

Entry information

Entry nameCWC2_YEAST
AccessionPrimary (citable) accession number: Q12046
Secondary accession number(s): D6VRE5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references