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Q12046

- CWC2_YEAST

UniProt

Q12046 - CWC2_YEAST

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Protein
Pre-mRNA-splicing factor CWC2
Gene
CWC2, NTC40, SLC3, YDL209C, D1041
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri67 – 9428C3H1-type
Add
BLAST

GO - Molecular functioni

  1. U6 snRNA binding Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. nucleotide binding Source: InterPro
  4. pre-mRNA binding Source: UniProtKB
  5. protein binding Source: IntAct

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. mRNA cis splicing, via spliceosome Source: UniProtKB
  3. mRNA splicing, via spliceosome Source: SGD
  4. positive regulation of RNA splicing Source: UniProtKB
  5. positive regulation of cell cycle Source: UniProtKB
  6. spliceosomal snRNP assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29591-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC2
Alternative name(s):
Complexed with CEF1 protein 2
PRP19-associated complex protein 40
Synthetic lethal with CLF1 protein 3
Gene namesi
Name:CWC2
Synonyms:NTC40, SLC3
Ordered Locus Names:YDL209C
ORF Names:D1041
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL209c.
SGDiS000002368. CWC2.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. Prp19 complex Source: SGD
  2. U2-type catalytic step 1 spliceosome Source: SGD
  3. U2-type catalytic step 2 spliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 731C → Y: Inhibits cell growth. 1 Publication
Mutagenesisi79 – 791G → D: No effect. Synthetic lethal when associated with CLF1 lacking a TPR domain. 1 Publication
Mutagenesisi87 – 871C → H: Inhibits cell growth. 1 Publication
Mutagenesisi186 – 1861F → D: Inhibits cell growth. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Pre-mRNA-splicing factor CWC2
PRO_0000081552Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei335 – 3351Phosphoserine1 Publication
Modified residuei336 – 3361Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12046.
PaxDbiQ12046.
PeptideAtlasiQ12046.

Expressioni

Gene expression databases

GenevestigatoriQ12046.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with ISY1 and PRP19.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP19P325239EBI-553,EBI-493

Protein-protein interaction databases

BioGridi31837. 59 interactions.
DIPiDIP-5164N.
IntActiQ12046. 50 interactions.
MINTiMINT-510131.
STRINGi4932.YDL209C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63
Helixi15 – 173
Beta strandi25 – 273
Helixi30 – 3910
Beta strandi41 – 455
Helixi55 – 584
Helixi63 – 664
Helixi74 – 774
Helixi84 – 863
Beta strandi88 – 925
Helixi96 – 10510
Beta strandi107 – 1093
Beta strandi113 – 1153
Helixi117 – 1193
Beta strandi125 – 1273
Helixi129 – 1335
Beta strandi136 – 1405
Helixi144 – 1463
Helixi153 – 16513
Beta strandi170 – 1767
Helixi177 – 1793
Beta strandi181 – 1888
Helixi189 – 19911
Helixi212 – 2176
Beta strandi222 – 2254

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TP2X-ray2.40A/B1-227[»]
3U1LX-ray1.64A1-240[»]
3U1MX-ray1.95A1-240[»]
ProteinModelPortaliQ12046.
SMRiQ12046. Positions 3-227.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini135 – 22894RRM
Add
BLAST

Domaini

The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding.

Sequence similaritiesi

Belongs to the RRM CWC2 family.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG288665.
HOGENOMiHOG000172735.
OMAiRIRYVES.
OrthoDBiEOG7D867D.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12046-1 [UniParc]FASTAAdd to Basket

« Hide

MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP    50
FALQPQLHSG KTRGDNDGQL FFCLFFAKGM CCLGPKCEYL HHIPDEEDIG 100
KLALRTEVLD CFGREKFADY REDMGGIGSF RKKNKTLYVG GIDGALNSKH 150
LKPAQIESRI RFVFSRLGDI DRIRYVESKN CGFVKFKYQA NAEFAKEAMS 200
NQTLLLPSDK EWDDRREGTG LLVKWANEDP DPAAQKRLQE ELKLESLNMM 250
VHLINNNTNS AGTEVNNKNN ERLDRTFPEA SVDNVKKRLL PLDNGMESDD 300
FIEKLKKVKK NISRENISSK PSVGKLGGPL LDYLSSDED 339
Length:339
Mass (Da):38,431
Last modified:November 1, 1996 - v1
Checksum:i371734A11DFDB339
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X99000 Genomic DNA. Translation: CAA67482.1.
Z74257 Genomic DNA. Translation: CAA98787.1.
AY693022 Genomic DNA. Translation: AAT93041.1.
BK006938 Genomic DNA. Translation: DAA11655.1.
PIRiS67768.
RefSeqiNP_010072.1. NM_001180269.1.

Genome annotation databases

EnsemblFungiiYDL209C; YDL209C; YDL209C.
GeneIDi851318.
KEGGisce:YDL209C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X99000 Genomic DNA. Translation: CAA67482.1 .
Z74257 Genomic DNA. Translation: CAA98787.1 .
AY693022 Genomic DNA. Translation: AAT93041.1 .
BK006938 Genomic DNA. Translation: DAA11655.1 .
PIRi S67768.
RefSeqi NP_010072.1. NM_001180269.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3TP2 X-ray 2.40 A/B 1-227 [» ]
3U1L X-ray 1.64 A 1-240 [» ]
3U1M X-ray 1.95 A 1-240 [» ]
ProteinModelPortali Q12046.
SMRi Q12046. Positions 3-227.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31837. 59 interactions.
DIPi DIP-5164N.
IntActi Q12046. 50 interactions.
MINTi MINT-510131.
STRINGi 4932.YDL209C.

Proteomic databases

MaxQBi Q12046.
PaxDbi Q12046.
PeptideAtlasi Q12046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL209C ; YDL209C ; YDL209C .
GeneIDi 851318.
KEGGi sce:YDL209C.

Organism-specific databases

CYGDi YDL209c.
SGDi S000002368. CWC2.

Phylogenomic databases

eggNOGi NOG288665.
HOGENOMi HOG000172735.
OMAi RIRYVES.
OrthoDBi EOG7D867D.

Enzyme and pathway databases

BioCyci YEAST:G3O-29591-MONOMER.

Miscellaneous databases

NextBioi 968354.

Gene expression databases

Genevestigatori Q12046.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR000571. Znf_CCCH.
[Graphical view ]
Pfami PF00076. RRM_1. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA."
    Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U., Schmidt E.R.
    Yeast 13:163-169(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p."
    Chen H.-R., Jan S.-P., Tsao T.Y., Sheu Y.-J., Banroques J., Cheng S.-C.
    Mol. Cell. Biol. 18:2196-2204(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRP19.
  6. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Characterization of interactions among the Cef1p-Prp19p-associated splicing complex."
    Ohi M.D., Gould K.L.
    RNA 8:798-815(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRP19 AND ISY1.
  8. "Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing."
    Vincent K., Wang Q., Jay S., Hobbs K., Rymond B.C.
    Genetics 164:895-907(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLY-79.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing."
    McGrail J.C., Krause A., O'Keefe R.T.
    Nucleic Acids Res. 37:4205-4217(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-73; CYS-87 AND PHE-186, RNA-BINDING.
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335 AND SER-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre."
    Rasche N., Dybkov O., Schmitzova J., Akyildiz B., Fabrizio P., Luhrmann R.
    EMBO J. 31:1591-1604(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, RNA-BINDING, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiCWC2_YEAST
AccessioniPrimary (citable) accession number: Q12046
Secondary accession number(s): D6VRE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2650 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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