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Q12046

- CWC2_YEAST

UniProt

Q12046 - CWC2_YEAST

Protein

Pre-mRNA-splicing factor CWC2

Gene

CWC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri67 – 9428C3H1-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. nucleotide binding Source: InterPro
    3. pre-mRNA binding Source: UniProtKB
    4. protein binding Source: IntAct
    5. U6 snRNA binding Source: UniProtKB

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. mRNA cis splicing, via spliceosome Source: UniProtKB
    3. mRNA splicing, via spliceosome Source: SGD
    4. positive regulation of cell cycle Source: UniProtKB
    5. positive regulation of RNA splicing Source: UniProtKB
    6. spliceosomal snRNP assembly Source: UniProtKB

    Keywords - Biological processi

    Cell cycle, mRNA processing, mRNA splicing

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29591-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor CWC2
    Alternative name(s):
    Complexed with CEF1 protein 2
    PRP19-associated complex protein 40
    Synthetic lethal with CLF1 protein 3
    Gene namesi
    Name:CWC2
    Synonyms:NTC40, SLC3
    Ordered Locus Names:YDL209C
    ORF Names:D1041
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL209c.
    SGDiS000002368. CWC2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. Prp19 complex Source: SGD
    2. U2-type catalytic step 1 spliceosome Source: SGD
    3. U2-type catalytic step 2 spliceosome Source: SGD

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi73 – 731C → Y: Inhibits cell growth. 1 Publication
    Mutagenesisi79 – 791G → D: No effect. Synthetic lethal when associated with CLF1 lacking a TPR domain. 1 Publication
    Mutagenesisi87 – 871C → H: Inhibits cell growth. 1 Publication
    Mutagenesisi186 – 1861F → D: Inhibits cell growth. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339Pre-mRNA-splicing factor CWC2PRO_0000081552Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei335 – 3351Phosphoserine1 Publication
    Modified residuei336 – 3361Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12046.
    PaxDbiQ12046.
    PeptideAtlasiQ12046.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12046.

    Interactioni

    Subunit structurei

    Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with ISY1 and PRP19.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PRP19P325239EBI-553,EBI-493

    Protein-protein interaction databases

    BioGridi31837. 59 interactions.
    DIPiDIP-5164N.
    IntActiQ12046. 50 interactions.
    MINTiMINT-510131.
    STRINGi4932.YDL209C.

    Structurei

    Secondary structure

    1
    339
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 63
    Helixi15 – 173
    Beta strandi25 – 273
    Helixi30 – 3910
    Beta strandi41 – 455
    Helixi55 – 584
    Helixi63 – 664
    Helixi74 – 774
    Helixi84 – 863
    Beta strandi88 – 925
    Helixi96 – 10510
    Beta strandi107 – 1093
    Beta strandi113 – 1153
    Helixi117 – 1193
    Beta strandi125 – 1273
    Helixi129 – 1335
    Beta strandi136 – 1405
    Helixi144 – 1463
    Helixi153 – 16513
    Beta strandi170 – 1767
    Helixi177 – 1793
    Beta strandi181 – 1888
    Helixi189 – 19911
    Helixi212 – 2176
    Beta strandi222 – 2254

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TP2X-ray2.40A/B1-227[»]
    3U1LX-ray1.64A1-240[»]
    3U1MX-ray1.95A1-240[»]
    ProteinModelPortaliQ12046.
    SMRiQ12046. Positions 3-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini135 – 22894RRMPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding.

    Sequence similaritiesi

    Belongs to the RRM CWC2 family.Curated
    Contains 1 C3H1-type zinc finger.PROSITE-ProRule annotation
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri67 – 9428C3H1-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG288665.
    HOGENOMiHOG000172735.
    OMAiRIRYVES.
    OrthoDBiEOG7D867D.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR000571. Znf_CCCH.
    [Graphical view]
    PfamiPF00076. RRM_1. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    PS50103. ZF_C3H1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12046-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP    50
    FALQPQLHSG KTRGDNDGQL FFCLFFAKGM CCLGPKCEYL HHIPDEEDIG 100
    KLALRTEVLD CFGREKFADY REDMGGIGSF RKKNKTLYVG GIDGALNSKH 150
    LKPAQIESRI RFVFSRLGDI DRIRYVESKN CGFVKFKYQA NAEFAKEAMS 200
    NQTLLLPSDK EWDDRREGTG LLVKWANEDP DPAAQKRLQE ELKLESLNMM 250
    VHLINNNTNS AGTEVNNKNN ERLDRTFPEA SVDNVKKRLL PLDNGMESDD 300
    FIEKLKKVKK NISRENISSK PSVGKLGGPL LDYLSSDED 339
    Length:339
    Mass (Da):38,431
    Last modified:November 1, 1996 - v1
    Checksum:i371734A11DFDB339
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99000 Genomic DNA. Translation: CAA67482.1.
    Z74257 Genomic DNA. Translation: CAA98787.1.
    AY693022 Genomic DNA. Translation: AAT93041.1.
    BK006938 Genomic DNA. Translation: DAA11655.1.
    PIRiS67768.
    RefSeqiNP_010072.1. NM_001180269.1.

    Genome annotation databases

    EnsemblFungiiYDL209C; YDL209C; YDL209C.
    GeneIDi851318.
    KEGGisce:YDL209C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99000 Genomic DNA. Translation: CAA67482.1 .
    Z74257 Genomic DNA. Translation: CAA98787.1 .
    AY693022 Genomic DNA. Translation: AAT93041.1 .
    BK006938 Genomic DNA. Translation: DAA11655.1 .
    PIRi S67768.
    RefSeqi NP_010072.1. NM_001180269.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TP2 X-ray 2.40 A/B 1-227 [» ]
    3U1L X-ray 1.64 A 1-240 [» ]
    3U1M X-ray 1.95 A 1-240 [» ]
    ProteinModelPortali Q12046.
    SMRi Q12046. Positions 3-227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31837. 59 interactions.
    DIPi DIP-5164N.
    IntActi Q12046. 50 interactions.
    MINTi MINT-510131.
    STRINGi 4932.YDL209C.

    Proteomic databases

    MaxQBi Q12046.
    PaxDbi Q12046.
    PeptideAtlasi Q12046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL209C ; YDL209C ; YDL209C .
    GeneIDi 851318.
    KEGGi sce:YDL209C.

    Organism-specific databases

    CYGDi YDL209c.
    SGDi S000002368. CWC2.

    Phylogenomic databases

    eggNOGi NOG288665.
    HOGENOMi HOG000172735.
    OMAi RIRYVES.
    OrthoDBi EOG7D867D.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29591-MONOMER.

    Miscellaneous databases

    NextBioi 968354.

    Gene expression databases

    Genevestigatori Q12046.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR000571. Znf_CCCH.
    [Graphical view ]
    Pfami PF00076. RRM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    PS50103. ZF_C3H1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA."
      Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U., Schmidt E.R.
      Yeast 13:163-169(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p."
      Chen H.-R., Jan S.-P., Tsao T.Y., Sheu Y.-J., Banroques J., Cheng S.-C.
      Mol. Cell. Biol. 18:2196-2204(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRP19.
    6. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Characterization of interactions among the Cef1p-Prp19p-associated splicing complex."
      Ohi M.D., Gould K.L.
      RNA 8:798-815(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRP19 AND ISY1.
    8. "Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing."
      Vincent K., Wang Q., Jay S., Hobbs K., Rymond B.C.
      Genetics 164:895-907(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF GLY-79.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing."
      McGrail J.C., Krause A., O'Keefe R.T.
      Nucleic Acids Res. 37:4205-4217(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-73; CYS-87 AND PHE-186, RNA-BINDING.
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335 AND SER-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre."
      Rasche N., Dybkov O., Schmitzova J., Akyildiz B., Fabrizio P., Luhrmann R.
      EMBO J. 31:1591-1604(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, RNA-BINDING, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiCWC2_YEAST
    AccessioniPrimary (citable) accession number: Q12046
    Secondary accession number(s): D6VRE5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 5, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2650 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3