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Protein

Pre-mRNA-splicing factor CWC2

Gene

CWC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.2 Publications

Miscellaneous

Present with 2650 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri67 – 94C3H1-typePROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • pre-mRNA binding Source: UniProtKB
  • U6 snRNA binding Source: UniProtKB

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • mRNA cis splicing, via spliceosome Source: UniProtKB
  • mRNA splicing, via spliceosome Source: SGD
  • positive regulation of cell cycle Source: UniProtKB
  • positive regulation of RNA splicing Source: UniProtKB
  • spliceosomal snRNP assembly Source: UniProtKB

Keywordsi

Molecular functionRNA-binding
Biological processCell cycle, mRNA processing, mRNA splicing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29591-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC2
Alternative name(s):
Complexed with CEF1 protein 2
PRP19-associated complex protein 40
Synthetic lethal with CLF1 protein 3
Gene namesi
Name:CWC2
Synonyms:NTC40, SLC3
Ordered Locus Names:YDL209C
ORF Names:D1041
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL209C
SGDiS000002368 CWC2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73C → Y: Inhibits cell growth. 1 Publication1
Mutagenesisi79G → D: No effect. Synthetic lethal when associated with CLF1 lacking a TPR domain. 1 Publication1
Mutagenesisi87C → H: Inhibits cell growth. 1 Publication1
Mutagenesisi186F → D: Inhibits cell growth. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000815521 – 339Pre-mRNA-splicing factor CWC2Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei335PhosphoserineCombined sources1
Modified residuei336PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12046
PaxDbiQ12046
PRIDEiQ12046

PTM databases

iPTMnetiQ12046

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with ISY1. Interacts with PRP19.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP19P3252313EBI-553,EBI-493

Protein-protein interaction databases

BioGridi31837, 109 interactors
DIPiDIP-5164N
IntActiQ12046, 51 interactors
STRINGi4932.YDL209C

Structurei

Secondary structure

1339
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Helixi15 – 17Combined sources3
Beta strandi25 – 27Combined sources3
Helixi30 – 39Combined sources10
Beta strandi41 – 45Combined sources5
Helixi55 – 58Combined sources4
Helixi63 – 66Combined sources4
Helixi74 – 77Combined sources4
Helixi84 – 86Combined sources3
Beta strandi88 – 92Combined sources5
Helixi96 – 105Combined sources10
Beta strandi107 – 109Combined sources3
Beta strandi113 – 115Combined sources3
Helixi117 – 119Combined sources3
Beta strandi125 – 127Combined sources3
Helixi129 – 133Combined sources5
Beta strandi136 – 140Combined sources5
Helixi144 – 146Combined sources3
Helixi153 – 165Combined sources13
Beta strandi170 – 176Combined sources7
Helixi177 – 179Combined sources3
Beta strandi181 – 188Combined sources8
Helixi189 – 199Combined sources11
Beta strandi207 – 209Combined sources3
Helixi212 – 217Combined sources6
Beta strandi222 – 225Combined sources4
Helixi232 – 255Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TP2X-ray2.40A/B1-227[»]
3U1LX-ray1.64A1-240[»]
3U1MX-ray1.95A1-240[»]
5GM6electron microscopy3.50R1-339[»]
5GMKelectron microscopy3.40R1-339[»]
5LJ3electron microscopy3.80M1-339[»]
5LJ5electron microscopy3.80M1-339[»]
5LQWelectron microscopy5.80F1-339[»]
5MPSelectron microscopy3.85M1-339[»]
5MQ0electron microscopy4.17M1-339[»]
5WSGelectron microscopy4.00R1-339[»]
6BK8electron microscopy3.30G1-339[»]
6EXNelectron microscopy3.70M1-339[»]
ProteinModelPortaliQ12046
SMRiQ12046
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini135 – 228RRMPROSITE-ProRule annotationAdd BLAST94

Domaini

The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding.

Sequence similaritiesi

Belongs to the RRM CWC2 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri67 – 94C3H1-typePROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000172735
InParanoidiQ12046
OMAiRIRYVES
OrthoDBiEOG092C4KFZ

Family and domain databases

CDDicd12360 RRM_cwf2, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR034181 Cwc2_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR032297 Torus
IPR000571 Znf_CCCH
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF16131 Torus, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50103 ZF_C3H1, 1 hit

Sequencei

Sequence statusi: Complete.

Q12046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP
60 70 80 90 100
FALQPQLHSG KTRGDNDGQL FFCLFFAKGM CCLGPKCEYL HHIPDEEDIG
110 120 130 140 150
KLALRTEVLD CFGREKFADY REDMGGIGSF RKKNKTLYVG GIDGALNSKH
160 170 180 190 200
LKPAQIESRI RFVFSRLGDI DRIRYVESKN CGFVKFKYQA NAEFAKEAMS
210 220 230 240 250
NQTLLLPSDK EWDDRREGTG LLVKWANEDP DPAAQKRLQE ELKLESLNMM
260 270 280 290 300
VHLINNNTNS AGTEVNNKNN ERLDRTFPEA SVDNVKKRLL PLDNGMESDD
310 320 330
FIEKLKKVKK NISRENISSK PSVGKLGGPL LDYLSSDED
Length:339
Mass (Da):38,431
Last modified:November 1, 1996 - v1
Checksum:i371734A11DFDB339
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99000 Genomic DNA Translation: CAA67482.1
Z74257 Genomic DNA Translation: CAA98787.1
AY693022 Genomic DNA Translation: AAT93041.1
BK006938 Genomic DNA Translation: DAA11655.1
PIRiS67768
RefSeqiNP_010072.1, NM_001180269.1

Genome annotation databases

EnsemblFungiiYDL209C; YDL209C; YDL209C
GeneIDi851318
KEGGisce:YDL209C

Similar proteinsi

Entry informationi

Entry nameiCWC2_YEAST
AccessioniPrimary (citable) accession number: Q12046
Secondary accession number(s): D6VRE5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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