Reviewed, UniProtKB/Swiss-Prot Q12045 (VIK1_YEAST)

Names and origin

Protein names

Recommended name:
    Spindle pole body-associated protein VIK1
Alternative name(s):
    Vegetative interaction with KAR3 protein 1

Gene names

Name:	VIK1
Ordered Locus Names:	YPL253C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Targets and/or maintains KAR3 at the spindle pole body during vegetative growth. Ref.2 Ref.3
Subunit structure	Interacts with KAR3. Ref.2
Subcellular location	Nucleus › spindle pole body. Nucleus. Ref.2 Ref.3

Ontologies

Keywords
Cellular component	Nucleus
Domain	Coiled coil
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	microtubule-based process Ref.2 Inferred from mutant phenotype. Source: SGD mitotic sister chromatid cohesion Inferred from genetic interaction. Source: SGD
Cellular component	kinesin complex Ref.2 Inferred from direct assay. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle pole body Ref.2 Ref.3 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-38784,EBI-24597
KAR3	P17119	3	EBI-38784,EBI-9499

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 647

647

Spindle pole body-associated protein VIK1

PRO_0000270929

Regions

Coiled coil

202 – 350

149

Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

647

Helix Strand Turn

Details...

Helix

353 – 372

20

Beta strand

376 – 381

6

Helix

383 – 385

3

Beta strand

390 – 393

4

Turn

394 – 397

4

Beta strand

398 – 401

4

Turn

402 – 404

3

Beta strand

407 – 409

3

Beta strand

411 – 415

5

Turn

416 – 418

3

Helix

421 – 427

7

Helix

430 – 438

9

Beta strand

443 – 448

6

Helix

454 – 465

12

Helix

470 – 474

5

Beta strand

475 – 485

11

Beta strand

490 – 492

3

Beta strand

506 – 509

4

Beta strand

514 – 518

5

Beta strand

521 – 525

5

Helix

526 – 529

4

Turn

532 – 535

4

Beta strand

546 – 557

12

Beta strand

570 – 577

8

Helix

580 – 591

12

Helix

599 – 610

12

Beta strand

614 – 619

6

Helix

622 – 624

3

Helix

625 – 638

14

Sequences

	Sequence		Length	Mass (Da)
	Q12045-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A6E61C51CC10B8EB	FASTA	647	75,730
	10 20 30 40 50 60 MASQQNKHAF LSKNRIFHNP DNVSSSKSRN LMDITNTTNT MNGSRPSSMK SSLALPPVKD 70 80 90 100 110 120 SFPSVSRSAS LNINMSKIKD LKDRQDKIRF QRHTLRTQLI ECEREIKTIK FRDLNKSRFE 130 140 150 160 170 180 LYKKKSKQAK YLKQVRDLTQ NLNSKDGERA DLIKKNKSAL ATLQAELDQN LILKRQESQE 190 200 210 220 230 240 LYNNKLIFWE NELQIMENVE PDHEITEEIS QLKKTLQELN INWANLQKQN LERQVNHESQ 250 260 270 280 290 300 LRKDFIAFKE AKLKSMENLT NKHRELLDQI ATLQSESEKL HKEIMDIDRQ AEYSEQNISE 310 320 330 340 350 360 INENIKQLEL ANNPLISKSL QNSQDLEHLQ NQMENLKEMA SKQEKFYNDT YNTVEKELLR 370 380 390 400 410 420 SRRLENSIIE QKGTMRCYAY VMEQNLPENL LFDYENGVIT QGLSEHVYKF NRVIPHLKVS 430 440 450 460 470 480 EDKFFTQEYS VYHDMCLNQK KNFNLISLST TPHGSLRESL IKFLAEKDTI YQKQYVITLQ 490 500 510 520 530 540 FVFLSDDEFS QDMLLDYSHN DKDSIKLKFE KHSISLDSKL VIIENGLEDL PLNFSCDEHP 550 560 570 580 590 600 NLPHSGMGII KVQFFPRDSK SDGNNDPVPV DFYFIELNNL KSIEQFDKSI FKKESCETPI 610 620 630 640 ALVLKKLISD TKSFFLLNLN DSKNVNKLLT ISEEVQTQLC KRKKKLT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p." Manning B.D., Barrett J.G., Wallace J.A., Granok H., Snyder M. J. Cell Biol. 144:1219-1233(1999) [PubMed: 10087265] [Abstract] Cited for: FUNCTION, INTERACTION WITH KAR3, SUBCELLULAR LOCATION.
[3]	"The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae." Shanks R.M.Q., Kamieniecki R.J., Dawson D.S. Genetics 159:939-951(2001) [PubMed: 11729143] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z73609 Genomic DNA. Translation: CAA97978.1.
Z67751 Genomic DNA. Translation: CAA91591.1.
Z73608 Genomic DNA. Translation: CAA97977.1.

PIR

S61011.

RefSeq

NP_015070.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O0A	X-ray	1.60	A	353-647	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2397N.

IntAct

Q12045. 9 interactions.

Genome annotation databases

Ensembl

YPL253C. Saccharomyces cerevisiae. [Contig view]

GeneID

855822.

GenomeReviews

Gene locus YPL253C in contig U00094_GR.

KEGG

sce:YPL253C.

NMPDR

fig|4932.3.peg.6196.

Organism-specific databases

CYGD

YPL253c.

SGD

S000006174. VIK1.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

Q12045.

OMA

Q12045. NISEINE.

Family and domain databases

ProtoNet

Search...

Other Resources

NextBio

980368.

Entry information

Entry name

VIK1_YEAST

Accession

Primary (citable) accession number: Q12045
Secondary accession number(s): Q7LGU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)