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Q12044 (RCN2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of calcineurin 2
Alternative name(s):
Weak suppressor of PAT1 ts protein 1
Gene names
Name:RCN2
Synonyms:WSP1
Ordered Locus Names:YOR220W
ORF Names:YOR50-10
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Cytoplasm Ref.5.

Post-translational modification

Phosphorylation of Ser-152 and Ser-160 is induced 2-fold in response to mating pheromone.

Miscellaneous

Present with 967 molecules/cell in log phase SD medium.

Ontologies

Keywords
   Cellular componentCytoplasm
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265Regulator of calcineurin 2
PRO_0000237641

Regions

Compositional bias141 – 20464Ser-rich

Amino acid modifications

Modified residue1041Phosphoserine Ref.8 Ref.10
Modified residue1101Phosphoserine Ref.9 Ref.10
Modified residue1321Phosphothreonine Ref.8 Ref.9 Ref.10
Modified residue1521Phosphoserine Ref.8 Ref.10
Modified residue1571Phosphoserine Ref.10
Modified residue1601Phosphoserine Ref.8 Ref.10
Modified residue1831Phosphoserine Ref.7 Ref.8 Ref.10
Modified residue1871Phosphoserine Ref.10
Modified residue1931Phosphoserine Ref.9 Ref.10
Modified residue2011Phosphoserine Ref.10
Modified residue2551Phosphoserine Ref.9 Ref.10

Sequences

Sequence LengthMass (Da)Tools
Q12044 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4A184C5C1218A617

FASTA26529,256
        10         20         30         40         50         60 
MANQKQMRTQ ILITDIPSGK FTSKWPTQLE KTLFKEQFPN LQSHLQYYTP LPFLNRIIII 

        70         80         90        100        110        120 
FDNEDDTLQV FKFLQELLAK ENSGPMKLFV TESLLNNQHP RSRSTDDAVS LQDNNLALLE 

       130        140        150        160        170        180 
DHRNKPLLSI NTDPGVTGVD SSSLNKGGSS LSPDKSSLES PTMLKLSTDS KPFSYQEPLP 

       190        200        210        220        230        240 
KLSRSSSSTS NLSLNRSSQT SLPSQLENKD KSASGTKCLF ASKPLGLTID TSTRSNAASC 

       250        260 
TENDVNATAS NPPKSPSITV NEFFH 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae."
Galisson F., Dujon B.
Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; THR-132; SER-152; SER-160 AND SER-183, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; THR-132; SER-193 AND SER-255, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-110; THR-132; SER-152; SER-157; SER-160; SER-183; SER-187; SER-193; SER-201 AND SER-255, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X92441 Genomic DNA. Translation: CAA63183.1.
Z75128 Genomic DNA. Translation: CAA99438.1.
AY557757 Genomic DNA. Translation: AAS56083.1.
BK006948 Genomic DNA. Translation: DAA10991.1.
PIRS60947.
RefSeqNP_014863.1. NM_001183639.1.

3D structure databases

ProteinModelPortalQ12044.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34614. 19 interactions.
DIPDIP-1996N.
IntActQ12044. 4 interactions.
MINTMINT-384402.
STRING4932.YOR220W.

Proteomic databases

MaxQBQ12044.
PaxDbQ12044.
PeptideAtlasQ12044.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR220W; YOR220W; YOR220W.
GeneID854395.
KEGGsce:YOR220W.

Organism-specific databases

CYGDYOR220w.
SGDS000005746. RCN2.

Phylogenomic databases

eggNOGNOG44665.
HOGENOMHOG000066002.
OMARCAREHY.
OrthoDBEOG7GTTGZ.

Enzyme and pathway databases

BioCycYEAST:G3O-33720-MONOMER.

Gene expression databases

GenevestigatorQ12044.

Family and domain databases

ProtoNetSearch...

Other

NextBio976559.

Entry information

Entry nameRCN2_YEAST
AccessionPrimary (citable) accession number: Q12044
Secondary accession number(s): D6W2S5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD