Regulator of calcineurin 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12044 (RCN2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Regulator of calcineurin 2

Alternative name(s):
Weak suppressor of PAT1 ts protein 1

Gene names

Name:	RCN2
Synonyms:	WSP1
Ordered Locus Names:	YOR220W
ORF Names:	YOR50-10

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasm Ref.7.
Post-translational modification	Phosphorylation of Ser-152 and Ser-160 is induced 2-fold in response to mating pheromone.
Miscellaneous	Present with 967 molecules/cell in log phase SD medium.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 265

265

Regulator of calcineurin 2

PRO_0000237641

Regions

Compositional bias

141 – 204

Ser-rich

Amino acid modifications

Modified residue

102

Phosphoserine Ref.10 Ref.11

Modified residue

104

Phosphoserine Ref.5 Ref.9 Ref.10 Ref.11

Modified residue

105

Phosphothreonine Ref.5 Ref.11

Modified residue

110

Phosphoserine Ref.10 Ref.11

Modified residue

132

Phosphothreonine Ref.9 Ref.10 Ref.11

Modified residue

150

Phosphoserine Ref.11

Modified residue

152

Phosphoserine Ref.5 Ref.6 Ref.9 Ref.10 Ref.11

Modified residue

156

Phosphoserine Ref.11

Modified residue

157

Phosphoserine Ref.5 Ref.11

Modified residue

160

Phosphoserine Ref.5 Ref.6 Ref.9 Ref.10 Ref.11

Modified residue

162

Phosphothreonine Ref.11

Modified residue

183

Phosphoserine Ref.5 Ref.9 Ref.10 Ref.11

Modified residue

185

Phosphoserine Ref.11

Modified residue

186

Phosphoserine Ref.10 Ref.11

Modified residue

187

Phosphoserine Ref.10 Ref.11

Modified residue

188

Phosphoserine Ref.11

Modified residue

189

Phosphothreonine Ref.10 Ref.11

Modified residue

193

Phosphoserine Ref.5 Ref.11

Modified residue

197

Phosphoserine Ref.11

Modified residue

198

Phosphoserine Ref.10 Ref.11

Modified residue

200

Phosphothreonine Ref.11

Modified residue

201

Phosphoserine Ref.10 Ref.11

Modified residue

255

Phosphoserine Ref.11

Modified residue

257

Phosphoserine Ref.10 Ref.11

Sequences

Sequence

Length

Mass (Da)

Tools

Q12044 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4A184C5C1218A617
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FASTA

265

29,256

        10         20         30         40         50         60 
MANQKQMRTQ ILITDIPSGK FTSKWPTQLE KTLFKEQFPN LQSHLQYYTP LPFLNRIIII 

        70         80         90        100        110        120 
FDNEDDTLQV FKFLQELLAK ENSGPMKLFV TESLLNNQHP RSRSTDDAVS LQDNNLALLE 

       130        140        150        160        170        180 
DHRNKPLLSI NTDPGVTGVD SSSLNKGGSS LSPDKSSLES PTMLKLSTDS KPFSYQEPLP 

       190        200        210        220        230        240 
KLSRSSSSTS NLSLNRSSQT SLPSQLENKD KSASGTKCLF ASKPLGLTID TSTRSNAASC 

       250        260 
TENDVNATAS NPPKSPSITV NEFFH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae." Galisson F., Dujon B. Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 102-123 AND 147-196, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; THR-105; SER-152; SER-157; SER-160; SER-183 AND SER-193, MASS SPECTROMETRY. Strain: YAL6B.
[6]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152 AND SER-160, MASS SPECTROMETRY. Strain: 2124.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; THR-132; SER-152; SER-160 AND SER-183, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102; SER-104; SER-110; THR-132; SER-152; SER-160; SER-183; SER-186; SER-187; THR-189; SER-198; SER-201 AND SER-257, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102; SER-104; THR-105; SER-110; THR-132; SER-150; SER-152; SER-156; SER-157; SER-160; THR-162; SER-183; SER-185; SER-186; SER-187; SER-188; THR-189; SER-193; SER-197; SER-198; THR-200; SER-201; SER-255 AND SER-257, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92441 Genomic DNA. Translation: CAA63183.1. Z75128 Genomic DNA. Translation: CAA99438.1. AY557757 Genomic DNA. Translation: AAS56083.1. BK006948 Genomic DNA. Translation: DAA10991.1.
PIR	S60947.
RefSeq	NP_014863.1. NM_001183639.1.
3D structure databases
ProteinModelPortal	Q12044.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1996N.
IntAct	Q12044. 6 interactions.
MINT	MINT-384402.
STRING	4932.YOR220W.
Proteomic databases
PaxDb	Q12044.
PeptideAtlas	Q12044.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR220W; YOR220W; YOR220W.
GeneID	854395.
KEGG	sce:YOR220W.
Organism-specific databases
CYGD	YOR220w.
SGD	S000005746. RCN2.
Phylogenomic databases
eggNOG	NOG44665.
HOGENOM	HOG000066002.
OMA	ANDEKAH.
OrthoDB	EOG48PQVZ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33720-MONOMER.
Gene expression databases
Genevestigator	Q12044.
GermOnline	YOR220W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	976559.

Entry information

Entry name

RCN2_YEAST

Accession

Primary (citable) accession number: Q12044
Secondary accession number(s): D6W2S5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents