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Protein

Lipase 5

Gene

TGL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Releases specific fatty acids from neutral lipid triacylglycerols (TAG) thereby supplying fatty acids to a general acylation process. May have a specific role in sporulation.1 Publication

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei216NucleophilePROSITE-ProRule annotation1
Active sitei375Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • lysophosphatidic acid acyltransferase activity Source: SGD
  • triglyceride lipase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Sporulation

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33618-MONOMER.
YEAST:G3O-33618-MONOMER.

Chemistry databases

SwissLipidsiSLP:000000054.
SLP:000000673.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipase 5 (EC:3.1.1.3)
Alternative name(s):
Triacylglycerol lipase 5
Gene namesi
Name:TGL5
Synonyms:STC2
Ordered Locus Names:YOR081C
ORF Names:YOR29-32, YOR2964C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR081C.
SGDiS000005607. TGL5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002709181 – 749Lipase 5Add BLAST749

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi270N-linked (GlcNAc...)Sequence analysis1
Glycosylationi289N-linked (GlcNAc...)Sequence analysis1
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1
Glycosylationi304N-linked (GlcNAc...)Sequence analysis1
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Glycosylationi474N-linked (GlcNAc...)Sequence analysis1
Glycosylationi589N-linked (GlcNAc...)Sequence analysis1
Modified residuei645PhosphoserineCombined sources1
Glycosylationi680N-linked (GlcNAc...)Sequence analysis1
Glycosylationi714N-linked (GlcNAc...)Sequence analysis1
Glycosylationi742N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ12043.
PRIDEiQ12043.

PTM databases

iPTMnetiQ12043.

Interactioni

Protein-protein interaction databases

BioGridi34480. 31 interactors.
DIPiDIP-6280N.
IntActiQ12043. 7 interactors.
MINTiMINT-608359.

Structurei

3D structure databases

ProteinModelPortaliQ12043.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini183 – 388PNPLAPROSITE-ProRule annotationAdd BLAST206

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi214 – 218GXSXGPROSITE-ProRule annotation5

Sequence similaritiesi

Contains 1 PNPLA (patatin-like phospholipase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016747.
HOGENOMiHOG000065996.
InParanoidiQ12043.
OMAiFRGHITI.
OrthoDBiEOG092C24S4.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
IPR021771. Triacylglycerol_lipase.
[Graphical view]
PfamiPF11815. DUF3336. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51635. PNPLA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTLPVTEF LLSKYYELSN TPATDSSSLF KWLYHKTLSR KQLLISDLSS
60 70 80 90 100
QKKHAISYDQ WNDIASRLDD LTGLSEWKTI DESSLYNYKL LQDLTIRMRH
110 120 130 140 150
LRTTHDYHRL LYLIRTKWVR NLGNMNNVNL YRHSHTGTKQ IIHDYLEESQ
160 170 180 190 200
AVLTALIHQS NMNDHYLLGI LQQTRRNIGR TALVLSGGST FGLFHIGVLA
210 220 230 240 250
ALFESDLMPK VISGSSAGAI VASIFCVHTT QEIPSLLTNV LNMEFNIFND
260 270 280 290 300
DNSKSPNENL LIKISRFCQN GTWFNNQPLI NTMLSFLGNL TFREAYNKTG
310 320 330 340 350
KILNITVSPA SIYEQPKLLN NLTAPNVLIW SAVCASCSLP GVFPSTPLFE
360 370 380 390 400
KDPHTGKIKE WGATNLHLSN MKFMDGSVDN DMPISRLSEM FNVDHIIACQ
410 420 430 440 450
VNIHVFPLLK FSNTCVGGEI EKEITARFRN QVTKIFKFFS DETIHFLDIL
460 470 480 490 500
KELEFHPYLM TKLKHLFLQQ YSGNVTILPD LSMVGQFHEV LKNPSQLFLL
510 520 530 540 550
HQTTLGARAT WPKISMIQNN CGQEFALDKA ITFLKEKIII SSSIKNPLQF
560 570 580 590 600
YQPRFSEQIK SLSIMDADLP GVDLEESSSN SLSIIKSPNK TAAPGRFPLQ
610 620 630 640 650
PLPSPSSTFN KRKMDMLSPS PSPSTSPQRS KSSFTQQGTR QKANSLSFAI
660 670 680 690 700
GASSLRLKKS PLKVPSRPQF KKRSSYYNQN MSAEMRKNRK KSGTISSYDV
710 720 730 740
QTNSEDFPIP AIENGSFDNT LFNPSRFPMD AMSAATNDNF MNNSDIFQN
Length:749
Mass (Da):84,716
Last modified:November 1, 1996 - v1
Checksum:iF3C5DBA97B6BA58B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64004.1.
Z74989 Genomic DNA. Translation: CAA99274.1.
Z70678 Genomic DNA. Translation: CAA94566.1.
BK006948 Genomic DNA. Translation: DAA10860.1.
PIRiS61643.
RefSeqiNP_014724.1. NM_001183500.1.

Genome annotation databases

EnsemblFungiiYOR081C; YOR081C; YOR081C.
GeneIDi854248.
KEGGisce:YOR081C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64004.1.
Z74989 Genomic DNA. Translation: CAA99274.1.
Z70678 Genomic DNA. Translation: CAA94566.1.
BK006948 Genomic DNA. Translation: DAA10860.1.
PIRiS61643.
RefSeqiNP_014724.1. NM_001183500.1.

3D structure databases

ProteinModelPortaliQ12043.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34480. 31 interactors.
DIPiDIP-6280N.
IntActiQ12043. 7 interactors.
MINTiMINT-608359.

Chemistry databases

SwissLipidsiSLP:000000054.
SLP:000000673.

PTM databases

iPTMnetiQ12043.

Proteomic databases

MaxQBiQ12043.
PRIDEiQ12043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR081C; YOR081C; YOR081C.
GeneIDi854248.
KEGGisce:YOR081C.

Organism-specific databases

EuPathDBiFungiDB:YOR081C.
SGDiS000005607. TGL5.

Phylogenomic databases

GeneTreeiENSGT00390000016747.
HOGENOMiHOG000065996.
InParanoidiQ12043.
OMAiFRGHITI.
OrthoDBiEOG092C24S4.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33618-MONOMER.
YEAST:G3O-33618-MONOMER.

Miscellaneous databases

PROiQ12043.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
IPR021771. Triacylglycerol_lipase.
[Graphical view]
PfamiPF11815. DUF3336. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51635. PNPLA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTGL5_YEAST
AccessioniPrimary (citable) accession number: Q12043
Secondary accession number(s): D6W2E4, Q7LH13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.