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Protein

Lipase 5

Gene

TGL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Releases specific fatty acids from neutral lipid triacylglycerols (TAG) thereby supplying fatty acids to a general acylation process. May have a specific role in sporulation.1 Publication

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei216NucleophilePROSITE-ProRule annotation1
Active sitei375Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • lysophosphatidic acid acyltransferase activity Source: SGD
  • triglyceride lipase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism, Sporulation

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33618-MONOMER
YEAST:G3O-33618-MONOMER

Chemistry databases

SwissLipidsiSLP:000000054
SLP:000000673

Names & Taxonomyi

Protein namesi
Recommended name:
Lipase 5 (EC:3.1.1.3)
Alternative name(s):
Triacylglycerol lipase 5
Gene namesi
Name:TGL5
Synonyms:STC2
Ordered Locus Names:YOR081C
ORF Names:YOR29-32, YOR2964C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR081C
SGDiS000005607 TGL5

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002709181 – 749Lipase 5Add BLAST749

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi270N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi289N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi304N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi321N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi474N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi589N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei645PhosphoserineCombined sources1
Glycosylationi680N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi714N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi742N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ12043
PaxDbiQ12043
PRIDEiQ12043

PTM databases

iPTMnetiQ12043

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34480, 63 interactors
DIPiDIP-6280N
IntActiQ12043, 31 interactors
MINTiQ12043
STRINGi4932.YOR081C

Structurei

3D structure databases

ProteinModelPortaliQ12043
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini183 – 388PNPLAPROSITE-ProRule annotationAdd BLAST206

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi214 – 218GXSXGPROSITE-ProRule annotation5

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016747
HOGENOMiHOG000065996
InParanoidiQ12043
OMAiILYSNFP
OrthoDBiEOG092C24S4

Family and domain databases

InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR002641 PNPLA_dom
IPR021771 Triacylglycerol_lipase
PfamiView protein in Pfam
PF11815 DUF3336, 1 hit
PF01734 Patatin, 1 hit
SUPFAMiSSF52151 SSF52151, 1 hit
PROSITEiView protein in PROSITE
PS51635 PNPLA, 1 hit

Sequencei

Sequence statusi: Complete.

Q12043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTLPVTEF LLSKYYELSN TPATDSSSLF KWLYHKTLSR KQLLISDLSS
60 70 80 90 100
QKKHAISYDQ WNDIASRLDD LTGLSEWKTI DESSLYNYKL LQDLTIRMRH
110 120 130 140 150
LRTTHDYHRL LYLIRTKWVR NLGNMNNVNL YRHSHTGTKQ IIHDYLEESQ
160 170 180 190 200
AVLTALIHQS NMNDHYLLGI LQQTRRNIGR TALVLSGGST FGLFHIGVLA
210 220 230 240 250
ALFESDLMPK VISGSSAGAI VASIFCVHTT QEIPSLLTNV LNMEFNIFND
260 270 280 290 300
DNSKSPNENL LIKISRFCQN GTWFNNQPLI NTMLSFLGNL TFREAYNKTG
310 320 330 340 350
KILNITVSPA SIYEQPKLLN NLTAPNVLIW SAVCASCSLP GVFPSTPLFE
360 370 380 390 400
KDPHTGKIKE WGATNLHLSN MKFMDGSVDN DMPISRLSEM FNVDHIIACQ
410 420 430 440 450
VNIHVFPLLK FSNTCVGGEI EKEITARFRN QVTKIFKFFS DETIHFLDIL
460 470 480 490 500
KELEFHPYLM TKLKHLFLQQ YSGNVTILPD LSMVGQFHEV LKNPSQLFLL
510 520 530 540 550
HQTTLGARAT WPKISMIQNN CGQEFALDKA ITFLKEKIII SSSIKNPLQF
560 570 580 590 600
YQPRFSEQIK SLSIMDADLP GVDLEESSSN SLSIIKSPNK TAAPGRFPLQ
610 620 630 640 650
PLPSPSSTFN KRKMDMLSPS PSPSTSPQRS KSSFTQQGTR QKANSLSFAI
660 670 680 690 700
GASSLRLKKS PLKVPSRPQF KKRSSYYNQN MSAEMRKNRK KSGTISSYDV
710 720 730 740
QTNSEDFPIP AIENGSFDNT LFNPSRFPMD AMSAATNDNF MNNSDIFQN
Length:749
Mass (Da):84,716
Last modified:November 1, 1996 - v1
Checksum:iF3C5DBA97B6BA58B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA Translation: CAA64004.1
Z74989 Genomic DNA Translation: CAA99274.1
Z70678 Genomic DNA Translation: CAA94566.1
BK006948 Genomic DNA Translation: DAA10860.1
PIRiS61643
RefSeqiNP_014724.1, NM_001183500.1

Genome annotation databases

EnsemblFungiiYOR081C; YOR081C; YOR081C
GeneIDi854248
KEGGisce:YOR081C

Similar proteinsi

Entry informationi

Entry nameiTGL5_YEAST
AccessioniPrimary (citable) accession number: Q12043
Secondary accession number(s): D6W2E4, Q7LH13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health