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Protein

ATP-dependent DNA helicase HMI1, mitochondrial

Gene

HMI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for mitochondrial genome maintenance and mitochondrial DNA inheritance.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei275ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 34ATPPROSITE-ProRule annotation6

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: SGD
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • mitochondrial DNA metabolic process Source: SGD
  • mitochondrial genome maintenance Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33495-MONOMER
BRENDAi3.6.4.12 984

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase HMI1, mitochondrial (EC:3.6.4.12)
Gene namesi
Name:HMI1
Ordered Locus Names:YOL095C
ORF Names:O0920
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YOL095C
SGDiS000005455 HMI1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi704R → D: Impaired import into mitochondrion. 1 Publication1
Mutagenesisi705R → D: Impaired import into mitochondrion. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000132971 – 692ATP-dependent DNA helicase HMI1, mitochondrialAdd BLAST692
PropeptideiPRO_0000013298693 – 706Cleaved upon import into mitochondrionAdd BLAST14

Proteomic databases

MaxQBiQ12039
PaxDbiQ12039
PRIDEiQ12039

PTM databases

iPTMnetiQ12039

Interactioni

Protein-protein interaction databases

BioGridi34307, 315 interactors
DIPiDIP-4225N
MINTiQ12039
STRINGi4932.YOL095C

Structurei

3D structure databases

ProteinModelPortaliQ12039
SMRiQ12039
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 277UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST273
Domaini278 – 593UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd BLAST316

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000066700
HOGENOMiHOG000112919
InParanoidiQ12039
KOiK19781
OMAiKHLTIAG
OrthoDBiEOG092C2AXC

Family and domain databases

InterProiView protein in InterPro
IPR014017 DNA_helicase_UvrD-like_C
IPR000212 DNA_helicase_UvrD/REP
IPR027417 P-loop_NTPase
IPR014016 UvrD-like_ATP-bd
PANTHERiPTHR11070 PTHR11070, 1 hit
PfamiView protein in Pfam
PF13361 UvrD_C, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51198 UVRD_HELICASE_ATP_BIND, 1 hit
PS51217 UVRD_HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKLTPSQWK VINKSYEPAS TIKVIAGPGS GKTLTLLYKV LHLITVENIK
60 70 80 90 100
PEEILIFSLT NKAVDSIIEN LLSIFENSHT NKEIVHQIGC YTVHGLANRI
110 120 130 140 150
VVENEGMINI IEEIGWRGLM KLLPPSKRTP HHFRSYKELE KVVKDYKLNN
160 170 180 190 200
AKNNNPVIEK LVELMDNCKV MTNDDLIIRA KKYLELDSSD SDASSFTQDL
210 220 230 240 250
RNKYKVVLID EFQDLYPSLA PLITMICKGK QLIMFGDTNQ SIYGFLGSNN
260 270 280 290 300
EIMSQLDNLH PKNSTTVLKL FDNFRSTPEI ISLASKIINR PLAEKQIIDD
310 320 330 340 350
TDETPSELVR KLPSGVSPQI MTFDDLAAES EFIIDKITQL ICSSAKFSDI
360 370 380 390 400
AILSRTNSHL TAIASILKKY GIPYQKLKSQ PDWMDDLRIQ FLLDILKVCS
410 420 430 440 450
LASDEKHNRE FNTGDKWQSN FSILVTMSAL KGIGDASIQA LYKACSLKNL
460 470 480 490 500
SIWKYLTMVP NFEWPLGLSI KKKMENYTSN LYEMIENDQV HQLDDPMELL
510 520 530 540 550
EKVASITNNL NLNPTYFQSL SDAQSSLEFK THLQEMAQVM KVSKSNKPPG
560 570 580 590 600
ISFVKWFLET YFDQTMVFHQ SQQALQTTGP GTVKLSTIHS AKGLEFPIVF
610 620 630 640 650
LTNGSMSNFP MDTNALYVGI TRARNLLYMC NMKHERLVSK SSPYSRNIMS
660 670 680 690 700
NNLFWTYYNK DLKRSVCDVK VTHGYNVQRY NQLRKNFGFY RAYSSLRGCK

SVFRRI
Length:706
Mass (Da):80,576
Last modified:November 1, 1996 - v1
Checksum:i626FD0FB2A5520F5
GO

Sequence cautioni

The sequence CAA88166 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26030 Genomic DNA Translation: AAC49064.1
Z48149 Genomic DNA Translation: CAA88166.1 Different initiation.
X83121 Genomic DNA Translation: CAA58184.1
Z74837 Genomic DNA Translation: CAA99107.1
BK006948 Genomic DNA Translation: DAA10689.1
PIRiS57374
RefSeqiNP_014546.1, NM_001183349.1

Genome annotation databases

EnsemblFungiiYOL095C; YOL095C; YOL095C
GeneIDi854058
KEGGisce:YOL095C

Similar proteinsi

Entry informationi

Entry nameiHMI1_YEAST
AccessioniPrimary (citable) accession number: Q12039
Secondary accession number(s): D6W1X3, Q05379
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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