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Protein

ATP-dependent DNA helicase HMI1, mitochondrial

Gene

HMI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for mitochondrial genome maintenance and mitochondrial DNA inheritance.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei275 – 2751ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 346ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: SGD
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • mitochondrial DNA metabolic process Source: SGD
  • mitochondrial genome maintenance Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33495-MONOMER.
BRENDAi3.6.4.12. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase HMI1, mitochondrial (EC:3.6.4.12)
Gene namesi
Name:HMI1
Ordered Locus Names:YOL095C
ORF Names:O0920
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL095C.
SGDiS000005455. HMI1.

Subcellular locationi

  • Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi704 – 7041R → D: Impaired import into mitochondrion. 1 Publication
Mutagenesisi705 – 7051R → D: Impaired import into mitochondrion. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692ATP-dependent DNA helicase HMI1, mitochondrialPRO_0000013297Add
BLAST
Propeptidei693 – 70614Cleaved upon import into mitochondrionPRO_0000013298Add
BLAST

Proteomic databases

MaxQBiQ12039.

PTM databases

iPTMnetiQ12039.

Interactioni

Protein-protein interaction databases

BioGridi34307. 98 interactions.
DIPiDIP-4225N.
MINTiMINT-509468.

Structurei

3D structure databases

ProteinModelPortaliQ12039.
SMRiQ12039. Positions 2-637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 277273UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini278 – 593316UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated
Contains 1 uvrD-like helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 uvrD-like helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000066700.
HOGENOMiHOG000112919.
InParanoidiQ12039.
KOiK19781.
OMAiQCIYEFL.
OrthoDBiEOG092C2AXC.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 3 hits.
PfamiPF00580. UvrD-helicase. 2 hits.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKLTPSQWK VINKSYEPAS TIKVIAGPGS GKTLTLLYKV LHLITVENIK
60 70 80 90 100
PEEILIFSLT NKAVDSIIEN LLSIFENSHT NKEIVHQIGC YTVHGLANRI
110 120 130 140 150
VVENEGMINI IEEIGWRGLM KLLPPSKRTP HHFRSYKELE KVVKDYKLNN
160 170 180 190 200
AKNNNPVIEK LVELMDNCKV MTNDDLIIRA KKYLELDSSD SDASSFTQDL
210 220 230 240 250
RNKYKVVLID EFQDLYPSLA PLITMICKGK QLIMFGDTNQ SIYGFLGSNN
260 270 280 290 300
EIMSQLDNLH PKNSTTVLKL FDNFRSTPEI ISLASKIINR PLAEKQIIDD
310 320 330 340 350
TDETPSELVR KLPSGVSPQI MTFDDLAAES EFIIDKITQL ICSSAKFSDI
360 370 380 390 400
AILSRTNSHL TAIASILKKY GIPYQKLKSQ PDWMDDLRIQ FLLDILKVCS
410 420 430 440 450
LASDEKHNRE FNTGDKWQSN FSILVTMSAL KGIGDASIQA LYKACSLKNL
460 470 480 490 500
SIWKYLTMVP NFEWPLGLSI KKKMENYTSN LYEMIENDQV HQLDDPMELL
510 520 530 540 550
EKVASITNNL NLNPTYFQSL SDAQSSLEFK THLQEMAQVM KVSKSNKPPG
560 570 580 590 600
ISFVKWFLET YFDQTMVFHQ SQQALQTTGP GTVKLSTIHS AKGLEFPIVF
610 620 630 640 650
LTNGSMSNFP MDTNALYVGI TRARNLLYMC NMKHERLVSK SSPYSRNIMS
660 670 680 690 700
NNLFWTYYNK DLKRSVCDVK VTHGYNVQRY NQLRKNFGFY RAYSSLRGCK

SVFRRI
Length:706
Mass (Da):80,576
Last modified:November 1, 1996 - v1
Checksum:i626FD0FB2A5520F5
GO

Sequence cautioni

The sequence CAA88166 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26030 Genomic DNA. Translation: AAC49064.1.
Z48149 Genomic DNA. Translation: CAA88166.1. Different initiation.
X83121 Genomic DNA. Translation: CAA58184.1.
Z74837 Genomic DNA. Translation: CAA99107.1.
BK006948 Genomic DNA. Translation: DAA10689.1.
PIRiS57374.
RefSeqiNP_014546.1. NM_001183349.1.

Genome annotation databases

EnsemblFungiiYOL095C; YOL095C; YOL095C.
GeneIDi854058.
KEGGisce:YOL095C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26030 Genomic DNA. Translation: AAC49064.1.
Z48149 Genomic DNA. Translation: CAA88166.1. Different initiation.
X83121 Genomic DNA. Translation: CAA58184.1.
Z74837 Genomic DNA. Translation: CAA99107.1.
BK006948 Genomic DNA. Translation: DAA10689.1.
PIRiS57374.
RefSeqiNP_014546.1. NM_001183349.1.

3D structure databases

ProteinModelPortaliQ12039.
SMRiQ12039. Positions 2-637.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34307. 98 interactions.
DIPiDIP-4225N.
MINTiMINT-509468.

PTM databases

iPTMnetiQ12039.

Proteomic databases

MaxQBiQ12039.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL095C; YOL095C; YOL095C.
GeneIDi854058.
KEGGisce:YOL095C.

Organism-specific databases

EuPathDBiFungiDB:YOL095C.
SGDiS000005455. HMI1.

Phylogenomic databases

GeneTreeiENSGT00530000066700.
HOGENOMiHOG000112919.
InParanoidiQ12039.
KOiK19781.
OMAiQCIYEFL.
OrthoDBiEOG092C2AXC.

Enzyme and pathway databases

BioCyciYEAST:G3O-33495-MONOMER.
BRENDAi3.6.4.12. 984.

Miscellaneous databases

PROiQ12039.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 3 hits.
PfamiPF00580. UvrD-helicase. 2 hits.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMI1_YEAST
AccessioniPrimary (citable) accession number: Q12039
Secondary accession number(s): D6W1X3, Q05379
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.