ID   SLF1_YEAST              Reviewed;         447 AA.
AC   Q12034; D6VTD6; E9P8Z6;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 173.
DE   RecName: Full=Protein SLF1;
GN   Name=SLF1; OrderedLocusNames=YDR515W; ORFNames=D9719.20;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8628314; DOI=10.1128/mcb.16.5.2464;
RA   Yu W., Farrell R.A., Stillman D.J., Winge D.R.;
RT   "Identification of SLF1 as a new copper homeostasis gene involved in copper
RT   sulfide mineralization in Saccharomyces cerevisiae.";
RL   Mol. Cell. Biol. 16:2464-2472(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Regulates the copper-dependent mineralization of copper
CC       sulfide complexes on the cell surface in cells cultured in medium
CC       containing copper salts.
CC   -!- MISCELLANEOUS: Present with 861 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; U30375; AAB04684.1; -; Genomic_DNA.
DR   EMBL; U33057; AAB64956.1; -; Genomic_DNA.
DR   EMBL; AY692939; AAT92958.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA12346.1; -; Genomic_DNA.
DR   PIR; S59742; S59742.
DR   RefSeq; NP_010803.3; NM_001180823.3.
DR   AlphaFoldDB; Q12034; -.
DR   SMR; Q12034; -.
DR   BioGRID; 32566; 581.
DR   DIP; DIP-2938N; -.
DR   IntAct; Q12034; 7.
DR   MINT; Q12034; -.
DR   STRING; 4932.YDR515W; -.
DR   iPTMnet; Q12034; -.
DR   MaxQB; Q12034; -.
DR   PaxDb; 4932-YDR515W; -.
DR   PeptideAtlas; Q12034; -.
DR   EnsemblFungi; YDR515W_mRNA; YDR515W; YDR515W.
DR   GeneID; 852127; -.
DR   KEGG; sce:YDR515W; -.
DR   AGR; SGD:S000002923; -.
DR   SGD; S000002923; SLF1.
DR   VEuPathDB; FungiDB:YDR515W; -.
DR   eggNOG; KOG2590; Eukaryota.
DR   GeneTree; ENSGT00940000176708; -.
DR   HOGENOM; CLU_039873_0_0_1; -.
DR   InParanoid; Q12034; -.
DR   OMA; EYAMESN; -.
DR   OrthoDB; 24041at2759; -.
DR   BioCyc; YEAST:G3O-30034-MONOMER; -.
DR   BioGRID-ORCS; 852127; 0 hits in 10 CRISPR screens.
DR   PRO; PR:Q12034; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; Q12034; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0010494; C:cytoplasmic stress granule; IDA:SGD.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0003729; F:mRNA binding; HDA:SGD.
DR   GO; GO:0003723; F:RNA binding; IDA:SGD.
DR   GO; GO:0006878; P:intracellular copper ion homeostasis; IMP:SGD.
DR   GO; GO:0045727; P:positive regulation of translation; IBA:GO_Central.
DR   GO; GO:0006417; P:regulation of translation; IMP:SGD.
DR   CDD; cd07323; LAM; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR045180; La_dom_prot.
DR   InterPro; IPR006630; La_HTH.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR22792:SF172; LUPUS LA PROTEIN HOMOLOG; 1.
DR   PANTHER; PTHR22792; LUPUS LA PROTEIN-RELATED; 1.
DR   Pfam; PF05383; La; 1.
DR   SMART; SM00715; LA; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS50961; HTH_LA; 1.
PE   1: Evidence at protein level;
KW   Copper; Reference proteome; RNA-binding.
FT   CHAIN           1..447
FT                   /note="Protein SLF1"
FT                   /id="PRO_0000207614"
FT   DOMAIN          265..368
FT                   /note="HTH La-type RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00332"
FT   REGION          1..143
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          157..198
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        27..48
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        61..102
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        122..143
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        118
FT                   /note="N -> S (in Ref. 4; AAT92958)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   447 AA;  50943 MW;  7D09BD00E141555B CRC64;
     MSSQNLNDNP KNTSSAAEDK KKQTSSLKLA PIPTTSPWKS SSPDSNTVIP VEELRDISKT
     AKPSKNGSGS IKLTSNTKWT PITPSVIISG SKDTNSKSGK NSKNSKTNKK MKKRGKYNND
     INKKDFNGQT NSTSEISNVS NLESKPLDAN AKVNIHSSSG ATANGNIKRI TNNNNSTNGR
     QSRNYQNRNG KTRYNNNSRH SQAANNAISF PNNYQARPEY IPNASHWLNN NSRNSYKQLS
     YFRQQQYYNN INYQQQLQTP YYYSMEPIFK SIESIKNQIE FYFSEENLKT DEFLRSKFKK
     ANDGFIPMSL IGKFYRMVNL SLGGDPNLIL ASMREVLQHK ETNHLEIALG SIEGAQKNMA
     DDFNPLENYF IRRENWAEYA MESNFDENDD ETEKYNIEKL LGPNDLDNYS YMGYPNFFPS
     NENGKKSQSY DQGEISRQFE QNLQIND
//