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Protein

Protein SLF1

Gene

SLF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the copper-dependent mineralization of copper sulfide complexes on the cell surface in cells cultured in medium containing copper salts.

GO - Molecular functioni

  • RNA binding Source: SGD

GO - Biological processi

  • cellular copper ion homeostasis Source: SGD
  • regulation of translation Source: SGD
Complete GO annotation...

Keywords - Ligandi

Copper, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30034-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SLF1
Gene namesi
Name:SLF1
Ordered Locus Names:YDR515W
ORF Names:D9719.20
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR515w.
EuPathDBiFungiDB:YDR515W.
SGDiS000002923. SLF1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic stress granule Source: SGD
  • polysome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Protein SLF1PRO_0000207614Add
BLAST

Proteomic databases

MaxQBiQ12034.
PaxDbiQ12034.

Expressioni

Gene expression databases

GenevestigatoriQ12034.

Interactioni

Protein-protein interaction databases

BioGridi32566. 533 interactions.
DIPiDIP-2938N.
IntActiQ12034. 5 interactions.
MINTiMINT-574898.
STRINGi4932.YDR515W.

Structurei

3D structure databases

ProteinModelPortaliQ12034.
SMRiQ12034. Positions 271-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini265 – 368104HTH La-type RNA-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH La-type RNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5193.
GeneTreeiENSGT00530000067375.
InParanoidiQ12034.
OrthoDBiEOG7QNVZG.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR006630. Lupus_La_RNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05383. La. 1 hit.
[Graphical view]
SMARTiSM00715. LA. 1 hit.
[Graphical view]
PROSITEiPS50961. HTH_LA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQNLNDNP KNTSSAAEDK KKQTSSLKLA PIPTTSPWKS SSPDSNTVIP
60 70 80 90 100
VEELRDISKT AKPSKNGSGS IKLTSNTKWT PITPSVIISG SKDTNSKSGK
110 120 130 140 150
NSKNSKTNKK MKKRGKYNND INKKDFNGQT NSTSEISNVS NLESKPLDAN
160 170 180 190 200
AKVNIHSSSG ATANGNIKRI TNNNNSTNGR QSRNYQNRNG KTRYNNNSRH
210 220 230 240 250
SQAANNAISF PNNYQARPEY IPNASHWLNN NSRNSYKQLS YFRQQQYYNN
260 270 280 290 300
INYQQQLQTP YYYSMEPIFK SIESIKNQIE FYFSEENLKT DEFLRSKFKK
310 320 330 340 350
ANDGFIPMSL IGKFYRMVNL SLGGDPNLIL ASMREVLQHK ETNHLEIALG
360 370 380 390 400
SIEGAQKNMA DDFNPLENYF IRRENWAEYA MESNFDENDD ETEKYNIEKL
410 420 430 440
LGPNDLDNYS YMGYPNFFPS NENGKKSQSY DQGEISRQFE QNLQIND
Length:447
Mass (Da):50,943
Last modified:November 1, 1996 - v1
Checksum:i7D09BD00E141555B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181N → S in AAT92958 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30375 Genomic DNA. Translation: AAB04684.1.
U33057 Genomic DNA. Translation: AAB64956.1.
AY692939 Genomic DNA. Translation: AAT92958.1.
BK006938 Genomic DNA. Translation: DAA12346.1.
PIRiS59742.
RefSeqiNP_010803.3. NM_001180823.3.

Genome annotation databases

EnsemblFungiiYDR515W; YDR515W; YDR515W.
GeneIDi852127.
KEGGisce:YDR515W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30375 Genomic DNA. Translation: AAB04684.1.
U33057 Genomic DNA. Translation: AAB64956.1.
AY692939 Genomic DNA. Translation: AAT92958.1.
BK006938 Genomic DNA. Translation: DAA12346.1.
PIRiS59742.
RefSeqiNP_010803.3. NM_001180823.3.

3D structure databases

ProteinModelPortaliQ12034.
SMRiQ12034. Positions 271-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32566. 533 interactions.
DIPiDIP-2938N.
IntActiQ12034. 5 interactions.
MINTiMINT-574898.
STRINGi4932.YDR515W.

Proteomic databases

MaxQBiQ12034.
PaxDbiQ12034.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR515W; YDR515W; YDR515W.
GeneIDi852127.
KEGGisce:YDR515W.

Organism-specific databases

CYGDiYDR515w.
EuPathDBiFungiDB:YDR515W.
SGDiS000002923. SLF1.

Phylogenomic databases

eggNOGiCOG5193.
GeneTreeiENSGT00530000067375.
InParanoidiQ12034.
OrthoDBiEOG7QNVZG.

Enzyme and pathway databases

BioCyciYEAST:G3O-30034-MONOMER.

Miscellaneous databases

NextBioi970519.
PROiQ12034.

Gene expression databases

GenevestigatoriQ12034.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR006630. Lupus_La_RNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05383. La. 1 hit.
[Graphical view]
SMARTiSM00715. LA. 1 hit.
[Graphical view]
PROSITEiPS50961. HTH_LA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of SLF1 as a new copper homeostasis gene involved in copper sulfide mineralization in Saccharomyces cerevisiae."
    Yu W., Farrell R.A., Stillman D.J., Winge D.R.
    Mol. Cell. Biol. 16:2464-2472(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSLF1_YEAST
AccessioniPrimary (citable) accession number: Q12034
Secondary accession number(s): D6VTD6, E9P8Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 861 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.