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Protein

Mitochondrial 2-methylisocitrate lyase

Gene

ICL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate during the metabolism of endogenous propionyl-CoA. Does not act on isocitrate.1 Publication

Miscellaneous

Present with 1084 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.

Pathwayi: propanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei238Curated1

GO - Molecular functioni

  • methylisocitrate lyase activity Source: SGD

GO - Biological processi

  • propionate catabolic process, 2-methylcitrate cycle Source: SGD

Keywordsi

Molecular functionLyase

Enzyme and pathway databases

BioCyciYEAST:G3O-34168-MONOMER.
UniPathwayiUPA00946.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial 2-methylisocitrate lyase (EC:4.1.3.30)
Short name:
Methylisocitrate lyase
Gene namesi
Name:ICL2
Ordered Locus Names:YPR006C
ORF Names:LPZ6C, YP9723.06C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR006C.
SGDiS000006210. ICL2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688001 – 575Mitochondrial 2-methylisocitrate lyaseAdd BLAST575

Proteomic databases

PRIDEiQ12031.

Expressioni

Inductioni

Repressed by glucose and induced by ethanol and threonine.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi36183. 40 interactors.
DIPiDIP-4593N.
IntActiQ12031. 1 interactor.
MINTiMINT-505141.
STRINGi4932.YPR006C.

Structurei

3D structure databases

ProteinModelPortaliQ12031.
SMRiQ12031.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000053163.
HOGENOMiHOG000238475.
InParanoidiQ12031.
KOiK01637.
OMAiMHDRKQW.
OrthoDBiEOG092C19UX.

Family and domain databases

InterProiView protein in InterPro
IPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
PANTHERiPTHR21631. PTHR21631. 1 hit.
PfamiView protein in Pfam
PF00463. ICL. 1 hit.
PIRSFiPIRSF001362. Isocit_lyase. 2 hits.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiView protein in PROSITE
PS00161. ISOCITRATE_LYASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12031-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITMINNKTF NRKTTGTLKK LVLSSDKSLR RSFNGASSTK DFVFSESSKV
60 70 80 90 100
EEWWESARFK NISRPYSATD VVKHRGSLPA NTSIYPSSYQ ARKLFNLLEE
110 120 130 140 150
NFKNGTPLHT LGVIDPVQMS QLARCRNIKV AYISGWACSS TLVGSTNEVS
160 170 180 190 200
PDFGDYPYDT VPNQVERIFK AQQLHDRKAF LEASIKGSTP VDYLKPIIAD
210 220 230 240 250
ADMGHGGPTT VMKVAKLFAE KGAAGIHLED QMVGGKRCGH LSGAVLVPTA
260 270 280 290 300
THLMRLISTR FQWDIMGTEN LVIARTDSCN GKLLSSSSDP RDHEFIRGII
310 320 330 340 350
RDNVVPWSEK LIEMEDKKIP NSAIADMEKE WYHENELFTF EEALEKQFTA
360 370 380 390 400
SEFESYKEKK EDLMVNKLGR AYLSLREMKL LAQEVTPLKK IIFDWDAPRT
410 420 430 440 450
KEGYYMFNGC IEAAIRRSLV FAPYSDMIWL ETKTPDLEQA RSFSRKIHKQ
460 470 480 490 500
LPATKLVYNL SPSFNWSAHG FDDKALKSFV WDLAKEGFTL QLVSLAGLHS
510 520 530 540 550
DGVSFWELAN SFQSDGMKAY VEKVQKREKE TNCDIMTHQL WSGAEYVDSL
560 570
MKVVQNGASS QTLSTSGESF TETQF
Length:575
Mass (Da):64,976
Last modified:November 1, 1997 - v1
Checksum:iC29A9A62A02BD291
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA95046.1.
Z48951 Genomic DNA. Translation: CAA88784.1.
U31900 Genomic DNA. Translation: AAA97585.1.
BK006949 Genomic DNA. Translation: DAA11433.1.
PIRiS52819.
RefSeqiNP_015331.1. NM_001184103.1.

Genome annotation databases

EnsemblFungiiYPR006C; YPR006C; YPR006C.
GeneIDi856114.
KEGGisce:YPR006C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiACEB_YEAST
AccessioniPrimary (citable) accession number: Q12031
Secondary accession number(s): D6W417
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 5, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names