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Protein

Mitochondrial 2-methylisocitrate lyase

Gene

ICL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate during the metabolism of endogenous propionyl-CoA. Does not act on isocitrate.1 Publication

Miscellaneous

Present with 1084 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.

Pathwayi: propanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei238Curated1

GO - Molecular functioni

  • methylisocitrate lyase activity Source: SGD

GO - Biological processi

  • propionate catabolic process, 2-methylcitrate cycle Source: SGD

Keywordsi

Molecular functionLyase

Enzyme and pathway databases

BioCyciYEAST:G3O-34168-MONOMER
UniPathwayiUPA00946

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial 2-methylisocitrate lyase (EC:4.1.3.30)
Short name:
Methylisocitrate lyase
Gene namesi
Name:ICL2
Ordered Locus Names:YPR006C
ORF Names:LPZ6C, YP9723.06C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR006C
SGDiS000006210 ICL2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688001 – 575Mitochondrial 2-methylisocitrate lyaseAdd BLAST575

Proteomic databases

PaxDbiQ12031
PRIDEiQ12031

Expressioni

Inductioni

Repressed by glucose and induced by ethanol and threonine.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi36183, 40 interactors
DIPiDIP-4593N
IntActiQ12031, 1 interactor
STRINGi4932.YPR006C

Structurei

3D structure databases

ProteinModelPortaliQ12031
SMRiQ12031
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000053163
HOGENOMiHOG000238475
InParanoidiQ12031
KOiK01637
OMAiMHDRKQW
OrthoDBiEOG092C19UX

Family and domain databases

InterProiView protein in InterPro
IPR006254 Isocitrate_lyase
IPR018523 Isocitrate_lyase_ph_CS
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR21631 PTHR21631, 1 hit
PfamiView protein in Pfam
PF00463 ICL, 1 hit
PIRSFiPIRSF001362 Isocit_lyase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR01346 isocit_lyase, 1 hit
PROSITEiView protein in PROSITE
PS00161 ISOCITRATE_LYASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q12031-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITMINNKTF NRKTTGTLKK LVLSSDKSLR RSFNGASSTK DFVFSESSKV
60 70 80 90 100
EEWWESARFK NISRPYSATD VVKHRGSLPA NTSIYPSSYQ ARKLFNLLEE
110 120 130 140 150
NFKNGTPLHT LGVIDPVQMS QLARCRNIKV AYISGWACSS TLVGSTNEVS
160 170 180 190 200
PDFGDYPYDT VPNQVERIFK AQQLHDRKAF LEASIKGSTP VDYLKPIIAD
210 220 230 240 250
ADMGHGGPTT VMKVAKLFAE KGAAGIHLED QMVGGKRCGH LSGAVLVPTA
260 270 280 290 300
THLMRLISTR FQWDIMGTEN LVIARTDSCN GKLLSSSSDP RDHEFIRGII
310 320 330 340 350
RDNVVPWSEK LIEMEDKKIP NSAIADMEKE WYHENELFTF EEALEKQFTA
360 370 380 390 400
SEFESYKEKK EDLMVNKLGR AYLSLREMKL LAQEVTPLKK IIFDWDAPRT
410 420 430 440 450
KEGYYMFNGC IEAAIRRSLV FAPYSDMIWL ETKTPDLEQA RSFSRKIHKQ
460 470 480 490 500
LPATKLVYNL SPSFNWSAHG FDDKALKSFV WDLAKEGFTL QLVSLAGLHS
510 520 530 540 550
DGVSFWELAN SFQSDGMKAY VEKVQKREKE TNCDIMTHQL WSGAEYVDSL
560 570
MKVVQNGASS QTLSTSGESF TETQF
Length:575
Mass (Da):64,976
Last modified:November 1, 1997 - v1
Checksum:iC29A9A62A02BD291
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA Translation: CAA95046.1
Z48951 Genomic DNA Translation: CAA88784.1
U31900 Genomic DNA Translation: AAA97585.1
BK006949 Genomic DNA Translation: DAA11433.1
PIRiS52819
RefSeqiNP_015331.1, NM_001184103.1

Genome annotation databases

EnsemblFungiiYPR006C; YPR006C; YPR006C
GeneIDi856114
KEGGisce:YPR006C

Similar proteinsi

Entry informationi

Entry nameiACEB_YEAST
AccessioniPrimary (citable) accession number: Q12031
Secondary accession number(s): D6W417
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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