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Protein

Transcription initiation factor TFIID subunit 10

Gene

TAF10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID and the transcription regulatory histone acetylation (HAT) complexes SAGA and SLIK. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.9 Publications

GO - Molecular functioni

  • chromatin binding Source: SGD
  • protein complex scaffold Source: SGD

GO - Biological processi

  • chromatin organization Source: SGD
  • histone acetylation Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29756-MONOMER.
ReactomeiR-SCE-5689880. Ub-specific processing proteases.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 10
Alternative name(s):
TAFII-23
Short name:
TAFII23
TAFII-25
Short name:
TAFII25
TBP-associated factor 10
TBP-associated factor 25 kDa
p25
Gene namesi
Name:TAF10
Synonyms:TAF23, TAF25
Ordered Locus Names:YDR167W
ORF Names:YD9489.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR167W.
SGDiS000002574. TAF10.

Subcellular locationi

GO - Cellular componenti

  • SAGA complex Source: SGD
  • SLIK (SAGA-like) complex Source: SGD
  • transcription factor TFIID complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001189011 – 206Transcription initiation factor TFIID subunit 10Add BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineCombined sources1
Modified residuei146PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12030.
PRIDEiQ12030.

PTM databases

iPTMnetiQ12030.

Interactioni

Subunit structurei

In TFIID, TAF10 heterodimerizes with TAF3 and TAF8. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Component of the 1.8 MDa SAGA complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA. Component of the SLIK complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SPT20, RTG2, TAF12, TAF6, HFI1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10 and TAF9.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-18889,EBI-18889
HFI1Q120607EBI-18889,EBI-8287
TAF11Q042265EBI-18889,EBI-18884
TAF12Q0376111EBI-18889,EBI-35097
TAF13P117478EBI-18889,EBI-18897
TAF6P5304010EBI-18889,EBI-18876
TAF8Q037507EBI-18889,EBI-27947

GO - Molecular functioni

  • protein complex scaffold Source: SGD

Protein-protein interaction databases

BioGridi32218. 76 interactors.
DIPiDIP-855N.
IntActiQ12030. 44 interactors.
MINTiMINT-405873.

Structurei

3D structure databases

ProteinModelPortaliQ12030.
SMRiQ12030.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 194Histone-foldAdd BLAST148

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi147 – 172Gln-richAdd BLAST26

Sequence similaritiesi

Belongs to the TAF10 family.Curated
Contains 1 histone-fold domain.Curated

Phylogenomic databases

GeneTreeiENSGT00390000009368.
HOGENOMiHOG000175811.
InParanoidiQ12030.
KOiK03134.
OMAiNTNNPMG.
OrthoDBiEOG092C3BLF.

Family and domain databases

CDDicd07982. TAF10. 1 hit.
InterProiIPR003923. TFIID_30kDa.
[Graphical view]
PANTHERiPTHR21242. PTHR21242. 1 hit.
PfamiPF03540. TFIID_30kDa. 1 hit.
[Graphical view]
PIRSFiPIRSF017246. TFIID_TAF10. 1 hit.
PRINTSiPR01443. TFIID30KDSUB.

Sequencei

Sequence statusi: Complete.

Q12030-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFEEDYDAE FDDNQEGQLE TPFPSVAGAD DGDNDNDDSV AENMKKKQKR
60 70 80 90 100
EAVVDDGSEN AFGIPEFTRK DKTLEEILEM MDSTPPIIPD AVIDYYLTKN
110 120 130 140 150
GFNVADVRVK RLLALATQKF VSDIAKDAYE YSRIRSSVAV SNANNSQARA
160 170 180 190 200
RQLLQGQQQP GVQQISQQQH QQNEKTTASK VVLTVNDLSS AVAEYGLNIG

RPDFYR
Length:206
Mass (Da):23,019
Last modified:November 1, 1996 - v1
Checksum:iA472CCA10DC3B495
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51636 Genomic DNA. Translation: AAB07766.1.
Z47813 Genomic DNA. Translation: CAA87798.1.
BK006938 Genomic DNA. Translation: DAA12007.1.
PIRiS50913.
RefSeqiNP_010451.3. NM_001180474.3.

Genome annotation databases

EnsemblFungiiYDR167W; YDR167W; YDR167W.
GeneIDi851745.
KEGGisce:YDR167W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51636 Genomic DNA. Translation: AAB07766.1.
Z47813 Genomic DNA. Translation: CAA87798.1.
BK006938 Genomic DNA. Translation: DAA12007.1.
PIRiS50913.
RefSeqiNP_010451.3. NM_001180474.3.

3D structure databases

ProteinModelPortaliQ12030.
SMRiQ12030.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32218. 76 interactors.
DIPiDIP-855N.
IntActiQ12030. 44 interactors.
MINTiMINT-405873.

PTM databases

iPTMnetiQ12030.

Proteomic databases

MaxQBiQ12030.
PRIDEiQ12030.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR167W; YDR167W; YDR167W.
GeneIDi851745.
KEGGisce:YDR167W.

Organism-specific databases

EuPathDBiFungiDB:YDR167W.
SGDiS000002574. TAF10.

Phylogenomic databases

GeneTreeiENSGT00390000009368.
HOGENOMiHOG000175811.
InParanoidiQ12030.
KOiK03134.
OMAiNTNNPMG.
OrthoDBiEOG092C3BLF.

Enzyme and pathway databases

BioCyciYEAST:G3O-29756-MONOMER.
ReactomeiR-SCE-5689880. Ub-specific processing proteases.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

PROiQ12030.

Family and domain databases

CDDicd07982. TAF10. 1 hit.
InterProiIPR003923. TFIID_30kDa.
[Graphical view]
PANTHERiPTHR21242. PTHR21242. 1 hit.
PfamiPF03540. TFIID_30kDa. 1 hit.
[Graphical view]
PIRSFiPIRSF017246. TFIID_TAF10. 1 hit.
PRINTSiPR01443. TFIID30KDSUB.
ProtoNetiSearch...

Entry informationi

Entry nameiTAF10_YEAST
AccessioniPrimary (citable) accession number: Q12030
Secondary accession number(s): D6VSE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.