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Protein

AP-1 complex subunit gamma-1

Gene

APL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The AP-1 complex interacts directly with clathrin.1 Publication

GO - Molecular functioni

  • clathrin binding Source: SGD
  • protein transporter activity Source: InterPro

GO - Biological processi

  • Golgi to vacuole transport Source: SGD
  • intracellular protein transport Source: InterPro
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34188-MONOMER.
ReactomeiR-SCE-432720. Lysosome Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit gamma-1
Alternative name(s):
Clathrin assembly protein complex 1 gamma large chain
Clathrin assembly protein large gamma chain
Gamma-adaptin
Gene namesi
Name:APL4
Ordered Locus Names:YPR029C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR029C.
SGDiS000006233. APL4.

Subcellular locationi

GO - Cellular componenti

  • AP-1 adaptor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 832832AP-1 complex subunit gamma-1PRO_0000193764Add
BLAST

Proteomic databases

MaxQBiQ12028.
PeptideAtlasiQ12028.

PTM databases

iPTMnetiQ12028.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). AP-1 interacts with clathrin.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
APL2P360006EBI-33025,EBI-2206
APS1P351816EBI-33025,EBI-2612

GO - Molecular functioni

  • clathrin binding Source: SGD

Protein-protein interaction databases

BioGridi36207. 101 interactions.
DIPiDIP-3913N.
IntActiQ12028. 29 interactions.
MINTiMINT-475384.

Structurei

3D structure databases

ProteinModelPortaliQ12028.
SMRiQ12028. Positions 5-632.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini733 – 832100GAEAdd
BLAST

Sequence similaritiesi

Contains 1 GAE domain.Curated

Phylogenomic databases

GeneTreeiENSGT00390000012618.
HOGENOMiHOG000210271.
InParanoidiQ12028.
KOiK12391.
OMAiNEKLVPQ.
OrthoDBiEOG7JHMDS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]
PIRSFiPIRSF037094. AP1_complex_gamma. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSLRSFIK DVRGAKTLAD ERAIITKQSA KIRTKLRDDH LPHEKRRVNI
60 70 80 90 100
QKLLYLYILG EKTHFGQVES INLIASDDFV DKRLGYLAAT LLLDESEDLL
110 120 130 140 150
TLLTNMLNND LHHPNKYAVS LALTSLGFLS SPELARDLYP DVENIIKNSR
160 170 180 190 200
DPFLLKKALQ CAAKLIFKDV SLLEIFNIED ITKILSSHSI CTHGVLLGVT
210 220 230 240 250
KIIQSILLIG LNRKKDEDED EDGIDYSNDI LSPLSLLLRD FFIRLENMNS
260 270 280 290 300
KNIEPGYDVQ GICDPFLQCE IIYTLKLYFQ VGELLNSNNV LDYKDNFCDL
310 320 330 340 350
LTRIATNTDS TKNSGQAILY ETVKTIFSLD LNQPLRVLGI NILAKFLAGK
360 370 380 390 400
DNNTKYVSLN TLLKVVPQEP TAVQRHRKFI SHCLQDTDVS IRMRALELSF
410 420 430 440 450
AILDDSNLVE LVNELMKFLA KQDEDSKDLI IYTIDHLIDT FDTRVVKDES
460 470 480 490 500
WKLDVFFNIL KLVGSFINYE KINDILIIIN NTSQLSDKSE FLRKMLTISL
510 520 530 540 550
NGTSAEISEE NIGWQLVLIW CIGEYGDLVL NEGNKNGADI INESSITDYL
560 570 580 590 600
LTLQELYTAT NLKIINYILT AALKLSVRFH DAKNIEKLRQ LILSYTDSTD
610 620 630 640 650
LSLQMKSNQY EIFFNQSISV KKIILETMPK FEKITEEQDN GKALSKNLIS
660 670 680 690 700
NEPVDLLSDL LGEDSKAESK ASTGDNVKPI DILEEIFGEK NDIAQVPKNA
710 720 730 740 750
NKEESINHSS AVEANSGVTL PLDANKIYDS SSLNVYASLL SANSGLAHLD
760 770 780 790 800
LYFQAKSLIS DLKTFCAVPK AQKLTLGQLY PSSTINASQI CKQSLKISGS
810 820 830
GKLKLRVKLD FHLNGSSSIT NEQFDHKFDE TL
Length:832
Mass (Da):93,624
Last modified:November 1, 1996 - v1
Checksum:iABB619E9DC4F565C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA95025.1.
Z49274 Genomic DNA. Translation: CAA89283.1.
BK006949 Genomic DNA. Translation: DAA11455.1.
PIRiS54503.
RefSeqiNP_015354.1. NM_001184126.1.

Genome annotation databases

EnsemblFungiiYPR029C; YPR029C; YPR029C.
GeneIDi856141.
KEGGisce:YPR029C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA95025.1.
Z49274 Genomic DNA. Translation: CAA89283.1.
BK006949 Genomic DNA. Translation: DAA11455.1.
PIRiS54503.
RefSeqiNP_015354.1. NM_001184126.1.

3D structure databases

ProteinModelPortaliQ12028.
SMRiQ12028. Positions 5-632.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36207. 101 interactions.
DIPiDIP-3913N.
IntActiQ12028. 29 interactions.
MINTiMINT-475384.

PTM databases

iPTMnetiQ12028.

Proteomic databases

MaxQBiQ12028.
PeptideAtlasiQ12028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR029C; YPR029C; YPR029C.
GeneIDi856141.
KEGGisce:YPR029C.

Organism-specific databases

EuPathDBiFungiDB:YPR029C.
SGDiS000006233. APL4.

Phylogenomic databases

GeneTreeiENSGT00390000012618.
HOGENOMiHOG000210271.
InParanoidiQ12028.
KOiK12391.
OMAiNEKLVPQ.
OrthoDBiEOG7JHMDS.

Enzyme and pathway databases

BioCyciYEAST:G3O-34188-MONOMER.
ReactomeiR-SCE-432720. Lysosome Vesicle Biogenesis.

Miscellaneous databases

PROiQ12028.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]
PIRSFiPIRSF037094. AP1_complex_gamma. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Adaptor complex-independent clathrin function in yeast."
    Yeung B.G., Phan H.L., Payne G.S.
    Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CLATHRIN.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAP1G1_YEAST
AccessioniPrimary (citable) accession number: Q12028
Secondary accession number(s): D6W439
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4850 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.