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Protein

Radiation-sensitive protein 28

Gene

RAD28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transcription-coupled repair nucleotide excision repair (NER) of UV-induced DNA lesions.2 Publications

GO - Biological processi

  • DNA repair Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-29645-MONOMER.
ReactomeiR-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Radiation-sensitive protein 28
Gene namesi
Name:RAD28
Ordered Locus Names:YDR030C
ORF Names:YD9813.08c
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR030C.
SGDiS000002437. RAD28.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Radiation-sensitive protein 28PRO_0000268697Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi32079. 9 interactions.
DIPiDIP-5177N.
MINTiMINT-573314.

Structurei

3D structure databases

ProteinModelPortaliQ12021.
SMRiQ12021. Positions 60-86, 193-386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati55 – 9440WD 1Add
BLAST
Repeati193 – 23341WD 2Add
BLAST
Repeati285 – 32541WD 3Add
BLAST
Repeati357 – 39640WD 4Add
BLAST
Repeati404 – 45148WD 5Add
BLAST

Sequence similaritiesi

Contains 5 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00390000009065.
HOGENOMiHOG000065962.
InParanoidiQ12021.
KOiK10570.
OMAiRMYRQSS.
OrthoDBiEOG7S226X.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12021-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPFLEFRVG NISLNEFYRR TIQSEFERIL EDPLSNMKNY RFSKQSNYST
60 70 80 90 100
KEKTPLSIGV NCLDIDDTGQ VLLGGGDDGS LSIWGLDESL HRNDEGEQEL
110 120 130 140 150
INKRLNYIKR QPHQSDDEPA QIMGYKNKRT RINDNNTMRL VHSFQTQRNK
160 170 180 190 200
YRMYRQSSAA VPVQRSHISN KTDSPIGFSE TLSETDSEAS ISHHKYGITT
210 220 230 240 250
LKWYKADNGM FFTGSNDKTV KIWDTNRFEA VQDINLGYKI NQIDNNVVDD
260 270 280 290 300
SSLLVVASED YYPRLIDLRT MNSGVTALGM GNQTRMQSEI LCCKFNPVRE
310 320 330 340 350
QIIACGDMEG GVKLWDLRMR NRLYSELKRN KNRFKTINND DNDDQSDVYF
360 370 380 390 400
SSNQSKAHLR CCSDIVWNSE GSELCSVGMD GKLNVWRPFT EILQPEGLAS
410 420 430 440 450
YSQLGTQDLS RIKYKKRVSR RLLWFDKFLL CITDNGEVEI YNTEEKKLWN
460 470 480 490 500
KLEYPMVNQV KKNQASHCQF SSMIVQTNIM NSVGLKLFFG TNNNTVSDGG

SIFECS
Length:506
Mass (Da):58,230
Last modified:November 1, 1996 - v1
Checksum:i3517F97D9C1F4874
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47814 Genomic DNA. Translation: CAA87809.1.
Z74326 Genomic DNA. Translation: CAA98852.1.
BK006938 Genomic DNA. Translation: DAA11874.1.
PIRiS50937.
RefSeqiNP_010313.3. NM_001180338.3.

Genome annotation databases

EnsemblFungiiYDR030C; YDR030C; YDR030C.
GeneIDi851594.
KEGGisce:YDR030C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47814 Genomic DNA. Translation: CAA87809.1.
Z74326 Genomic DNA. Translation: CAA98852.1.
BK006938 Genomic DNA. Translation: DAA11874.1.
PIRiS50937.
RefSeqiNP_010313.3. NM_001180338.3.

3D structure databases

ProteinModelPortaliQ12021.
SMRiQ12021. Positions 60-86, 193-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32079. 9 interactions.
DIPiDIP-5177N.
MINTiMINT-573314.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR030C; YDR030C; YDR030C.
GeneIDi851594.
KEGGisce:YDR030C.

Organism-specific databases

EuPathDBiFungiDB:YDR030C.
SGDiS000002437. RAD28.

Phylogenomic databases

GeneTreeiENSGT00390000009065.
HOGENOMiHOG000065962.
InParanoidiQ12021.
KOiK10570.
OMAiRMYRQSS.
OrthoDBiEOG7S226X.

Enzyme and pathway databases

BioCyciYEAST:G3O-29645-MONOMER.
ReactomeiR-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Miscellaneous databases

NextBioi969078.
PROiQ12021.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Molecular cloning and characterization of Saccharomyces cerevisiae RAD28, the yeast homolog of the human Cockayne syndrome A (CSA) gene."
    Bhatia P.K., Verhage R.A., Brouwer J., Friedberg E.C.
    J. Bacteriol. 178:5977-5988(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Recovery of RNA polymerase II synthesis following DNA damage in mutants of Saccharomyces cerevisiae defective in nucleotide excision repair."
    Reagan M.S., Friedberg E.C.
    Nucleic Acids Res. 25:4257-4263(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAD28_YEAST
AccessioniPrimary (citable) accession number: Q12021
Secondary accession number(s): D6VS14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.