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Protein

Probable palmitoyltransferase AKR2

Gene

AKR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in constitutive endocytosis of a-factor receptor STE3.1 Publication

Miscellaneous

Present with 815 molecules/cell in log phase SD medium.1 Publication

Caution

It is uncertain whether Met-1 or Met-4 is the initiator.Curated

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei476S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

  • palmitoyltransferase activity Source: SGD
  • protein-cysteine S-palmitoyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  • protein palmitoylation Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-33580-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable palmitoyltransferase AKR2 (EC:2.3.1.225)
Alternative name(s):
Ankyrin repeat-containing protein AKR2
Gene namesi
Name:AKR2
Ordered Locus Names:YOR034C
ORF Names:OR26.25
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR034C
SGDiS000005560 AKR2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 306CytoplasmicSequence analysisAdd BLAST306
Transmembranei307 – 327HelicalSequence analysisAdd BLAST21
Transmembranei328 – 348HelicalSequence analysisAdd BLAST21
Topological domaini349 – 360CytoplasmicSequence analysisAdd BLAST12
Transmembranei361 – 381HelicalSequence analysisAdd BLAST21
Topological domaini382 – 392ExtracellularSequence analysisAdd BLAST11
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 489CytoplasmicSequence analysisAdd BLAST76
Transmembranei490 – 510HelicalSequence analysisAdd BLAST21
Topological domaini511 – 546ExtracellularSequence analysisAdd BLAST36
Transmembranei547 – 567HelicalSequence analysisAdd BLAST21
Topological domaini568 – 749CytoplasmicSequence analysisAdd BLAST182

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002129361 – 749Probable palmitoyltransferase AKR2Add BLAST749

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi534N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiQ12013
PaxDbiQ12013
PRIDEiQ12013

PTM databases

iPTMnetiQ12013

Interactioni

Protein-protein interaction databases

BioGridi34436, 72 interactors
DIPiDIP-1882N
IntActiQ12013, 17 interactors
MINTiQ12013
STRINGi4932.YOR034C

Structurei

3D structure databases

ProteinModelPortaliQ12013
SMRiQ12013
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati49 – 79ANK 1Add BLAST31
Repeati83 – 112ANK 2Add BLAST30
Repeati117 – 146ANK 3Add BLAST30
Repeati150 – 183ANK 4Add BLAST34
Repeati189 – 218ANK 5Add BLAST30
Repeati222 – 251ANK 6Add BLAST30
Domaini446 – 496DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063074
HOGENOMiHOG000033883
InParanoidiQ12013
KOiK20032
OrthoDBiEOG092C216R

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR001594 Palmitoyltrfase_DHHC
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF01529 DHHC, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits
PS50216 DHHC, 1 hit

Sequencei

Sequence statusi: Complete.

Q12013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSMSIIDDE NVKKTSNGAA VVTDVAQHAV SDSDNNKAQL LGDGSNTEYV
60 70 80 90 100
VDIFIEAAKD GDLKVVKDVV ESGAVDINND RIDELSGLHW ACINNRFSVA
110 120 130 140 150
KFLLLRGANP NQAAGPGGAT ALHWAARYGN IYIVDLLLKH GADPTLKDEQ
160 170 180 190 200
GLNIMHFSVY SSNILLVVYV LYFVVNNNDN VDIDSKDNNN RTPLLWAAYQ
210 220 230 240 250
GDFLTVELLL KFGSTVAWTD NRGFNALHCA LVGGDQRVIC DLILSGANFY
260 270 280 290 300
ERNNQKQDCF DLAEGMGTKS LFEQALQHHG YDRLGNQKDK LFKKSSHAQF
310 320 330 340 350
TIFLSPFLLM VYIYLISLVL SPVLAIMLSL LVTVVMVNTL KKFVLPCLPR
360 370 380 390 400
KNTYKVSLTR TPFFSGLFLS TFCFLIYIWT KKLYPYSVSD YTMKNVQFLV
410 420 430 440 450
TSFLTVVLFL RLVRSDPGCL KTDDSLTSIQ ETIKQLIDLG KFDRENFCVE
460 470 480 490 500
TLERKPLRSK YSFFSGALVA RYDHYCPWIY NDVGLKNHKL FVFFAVTVQY
510 520 530 540 550
HMFLFMWLCL AYFKKTNYIY EQVEEYARCA LLKNETLCKG SNYDPSTFFL
560 570 580 590 600
FIWISVNFIW LGAMLIVQFF QILKGITTPE LFILIKEEHK AKFINLIPFE
610 620 630 640 650
NSIYTSESKG VEDSDMIPEG PSATTITHTI SIDGLEPRNR RRAILSACFS
660 670 680 690 700
MMGINQWLVT IKEIVGITHI LHGQVPQQHH SSLLRSFLVT NHWKTNLTDF
710 720 730 740
WLNSDVTAPL WQRFFYSSDT SKAMLGGTEV DYYELYEYPA REGEVLRPN
Length:749
Mass (Da):85,236
Last modified:November 1, 1996 - v1
Checksum:i887F5A02B9EE030A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87331 Genomic DNA Translation: CAA60751.1
Z74942 Genomic DNA Translation: CAA99224.1
BK006948 Genomic DNA Translation: DAA10816.1
PIRiS62171
RefSeqiNP_014677.1, NM_001183453.1

Genome annotation databases

EnsemblFungiiYOR034C; YOR034C; YOR034C
GeneIDi854199
KEGGisce:YOR034C

Similar proteinsi

Entry informationi

Entry nameiAKR2_YEAST
AccessioniPrimary (citable) accession number: Q12013
Secondary accession number(s): D6W2A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health