Reviewed, UniProtKB/Swiss-Prot Q12013 (AKR2_YEAST)

Names and origin

Protein names

Recommended name:
    Probable palmitoyltransferase AKR2
    EC=2.3.1.-
Alternative name(s):
    Ankyrin repeat-containing protein AKR2

Gene names

Name:	AKR2
Ordered Locus Names:	YOR034C
ORF Names:	OR26.25

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	749 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in constitutive endocytosis of a-factor receptor STE3. Ref.2
Catalytic activity	Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	The DHHC domain is required for palmitoyltransferase activity By similarity.
Miscellaneous	Present with 815 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily. Contains 6 ANK repeats. Contains 1 DHHC-type zinc finger.
Caution	It is uncertain whether Met-1 or Met-4 is the initiator.

Ontologies

Keywords
Cellular component	Membrane
Domain	ANK repeat Repeat Transmembrane Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LCB4	Q12246	1	EBI-34438,EBI-38830
YPT35	P38815	1	EBI-34438,EBI-24665

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 749	749	Probable palmitoyltransferase AKR2		PRO_0000212936
Regions
	Topological domain	1 – 306	306	Cytoplasmic Potential
	Transmembrane	307 – 327	21	Potential
	Transmembrane	328 – 348	21	Potential
	Topological domain	349 – 360	12	Cytoplasmic Potential
	Transmembrane	361 – 381	21	Potential
	Topological domain	382 – 392	11	Extracellular Potential
	Transmembrane	393 – 413	21	Potential
	Topological domain	414 – 489	76	Cytoplasmic Potential
	Transmembrane	490 – 510	21	Potential
	Topological domain	511 – 546	36	Extracellular Potential
	Transmembrane	547 – 567	21	Potential
	Topological domain	568 – 749	182	Cytoplasmic Potential
	Repeat	49 – 79	31	ANK 1
	Repeat	83 – 112	30	ANK 2
	Repeat	117 – 146	30	ANK 3
	Repeat	150 – 183	34	ANK 4
	Repeat	189 – 218	30	ANK 5
	Repeat	222 – 251	30	ANK 6
	Zinc finger	446 – 496	51	DHHC-type
Sites
	Active site	476	1	S-palmitoyl cysteine intermediate By similarity
Amino acid modifications
	Glycosylation	534	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12013-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 887F5A02B9EE030A	FASTA	749	85,236
	10 20 30 40 50 60 MTSMSIIDDE NVKKTSNGAA VVTDVAQHAV SDSDNNKAQL LGDGSNTEYV VDIFIEAAKD 70 80 90 100 110 120 GDLKVVKDVV ESGAVDINND RIDELSGLHW ACINNRFSVA KFLLLRGANP NQAAGPGGAT 130 140 150 160 170 180 ALHWAARYGN IYIVDLLLKH GADPTLKDEQ GLNIMHFSVY SSNILLVVYV LYFVVNNNDN 190 200 210 220 230 240 VDIDSKDNNN RTPLLWAAYQ GDFLTVELLL KFGSTVAWTD NRGFNALHCA LVGGDQRVIC 250 260 270 280 290 300 DLILSGANFY ERNNQKQDCF DLAEGMGTKS LFEQALQHHG YDRLGNQKDK LFKKSSHAQF 310 320 330 340 350 360 TIFLSPFLLM VYIYLISLVL SPVLAIMLSL LVTVVMVNTL KKFVLPCLPR KNTYKVSLTR 370 380 390 400 410 420 TPFFSGLFLS TFCFLIYIWT KKLYPYSVSD YTMKNVQFLV TSFLTVVLFL RLVRSDPGCL 430 440 450 460 470 480 KTDDSLTSIQ ETIKQLIDLG KFDRENFCVE TLERKPLRSK YSFFSGALVA RYDHYCPWIY 490 500 510 520 530 540 NDVGLKNHKL FVFFAVTVQY HMFLFMWLCL AYFKKTNYIY EQVEEYARCA LLKNETLCKG 550 560 570 580 590 600 SNYDPSTFFL FIWISVNFIW LGAMLIVQFF QILKGITTPE LFILIKEEHK AKFINLIPFE 610 620 630 640 650 660 NSIYTSESKG VEDSDMIPEG PSATTITHTI SIDGLEPRNR RRAILSACFS MMGINQWLVT 670 680 690 700 710 720 IKEIVGITHI LHGQVPQQHH SSLLRSFLVT NHWKTNLTDF WLNSDVTAPL WQRFFYSSDT 730 740 SKAMLGGTEV DYYELYEYPA REGEVLRPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The ankyrin repeat-containing protein Akr1p is required for the endocytosis of yeast pheromone receptors." Givan S.A., Sprague G.F. Jr. Mol. Biol. Cell 8:1317-1327(1997) [PubMed: 9243510] [Abstract] Cited for: FUNCTION.
[3]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X87331 Genomic DNA. Translation: CAA60751.1. Z74942 Genomic DNA. Translation: CAA99224.1.
PIR	S62171.
RefSeq	NP_014677.1.
3D structure databases
HSSP	HSSP built from PDB template 1SW6 based on UniProtKB P09959.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1882N.
IntAct	Q12013. 8 interactions.
Genome annotation databases
Ensembl	YOR034C. Saccharomyces cerevisiae. [Contig view]
GeneID	854199.
GenomeReviews	Gene locus YOR034C in contig Y13140_GR.
KEGG	sce:YOR034C.
NMPDR	fig|4932.3.peg.5776.
Organism-specific databases
CYGD	YOR034c.
SGD	S000005560. AKR2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q12013.
OMA	Q12013. TITHTIS.
Gene expression databases
ArrayExpress	Q12013.
GermOnline	YOR034C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002110. ANK. IPR001594. Znf_DHHC. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00023. Ank. 4 hits. PF01529. zf-DHHC. 1 hit. [Graphical view]
ProDom	PD003041. Znf_DHHC. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00248. ANK. 6 hits. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 4 hits. PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976032.

Entry information

Entry name

AKR2_YEAST

Accession

Primary (citable) accession number: Q12013

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)