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Protein

Probable palmitoyltransferase AKR2

Gene

AKR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in constitutive endocytosis of a-factor receptor STE3.1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei476 – 4761S-palmitoyl cysteine intermediateBy similarity

GO - Molecular functioni

  • palmitoyltransferase activity Source: SGD
  • protein-cysteine S-palmitoyltransferase activity Source: UniProtKB-EC
  • zinc ion binding Source: InterPro

GO - Biological processi

  • protein palmitoylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-33580-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable palmitoyltransferase AKR2 (EC:2.3.1.225)
Alternative name(s):
Ankyrin repeat-containing protein AKR2
Gene namesi
Name:AKR2
Ordered Locus Names:YOR034C
ORF Names:OR26.25
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR034C.
SGDiS000005560. AKR2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 306306CytoplasmicSequence analysisAdd
BLAST
Transmembranei307 – 32721HelicalSequence analysisAdd
BLAST
Transmembranei328 – 34821HelicalSequence analysisAdd
BLAST
Topological domaini349 – 36012CytoplasmicSequence analysisAdd
BLAST
Transmembranei361 – 38121HelicalSequence analysisAdd
BLAST
Topological domaini382 – 39211ExtracellularSequence analysisAdd
BLAST
Transmembranei393 – 41321HelicalSequence analysisAdd
BLAST
Topological domaini414 – 48976CytoplasmicSequence analysisAdd
BLAST
Transmembranei490 – 51021HelicalSequence analysisAdd
BLAST
Topological domaini511 – 54636ExtracellularSequence analysisAdd
BLAST
Transmembranei547 – 56721HelicalSequence analysisAdd
BLAST
Topological domaini568 – 749182CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 749749Probable palmitoyltransferase AKR2PRO_0000212936Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi534 – 5341N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiQ12013.

PTM databases

iPTMnetiQ12013.

Interactioni

Protein-protein interaction databases

BioGridi34436. 20 interactions.
DIPiDIP-1882N.
IntActiQ12013. 6 interactions.
MINTiMINT-395171.

Structurei

3D structure databases

ProteinModelPortaliQ12013.
SMRiQ12013. Positions 17-264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati49 – 7931ANK 1Add
BLAST
Repeati83 – 11230ANK 2Add
BLAST
Repeati117 – 14630ANK 3Add
BLAST
Repeati150 – 18334ANK 4Add
BLAST
Repeati189 – 21830ANK 5Add
BLAST
Repeati222 – 25130ANK 6Add
BLAST
Domaini446 – 49651DHHCPROSITE-ProRule annotationAdd
BLAST

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 DHHC domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063074.
HOGENOMiHOG000033883.
InParanoidiQ12013.
KOiK20032.
OMAiCIMAVIN.
OrthoDBiEOG092C216R.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF01529. zf-DHHC. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS50216. DHHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSMSIIDDE NVKKTSNGAA VVTDVAQHAV SDSDNNKAQL LGDGSNTEYV
60 70 80 90 100
VDIFIEAAKD GDLKVVKDVV ESGAVDINND RIDELSGLHW ACINNRFSVA
110 120 130 140 150
KFLLLRGANP NQAAGPGGAT ALHWAARYGN IYIVDLLLKH GADPTLKDEQ
160 170 180 190 200
GLNIMHFSVY SSNILLVVYV LYFVVNNNDN VDIDSKDNNN RTPLLWAAYQ
210 220 230 240 250
GDFLTVELLL KFGSTVAWTD NRGFNALHCA LVGGDQRVIC DLILSGANFY
260 270 280 290 300
ERNNQKQDCF DLAEGMGTKS LFEQALQHHG YDRLGNQKDK LFKKSSHAQF
310 320 330 340 350
TIFLSPFLLM VYIYLISLVL SPVLAIMLSL LVTVVMVNTL KKFVLPCLPR
360 370 380 390 400
KNTYKVSLTR TPFFSGLFLS TFCFLIYIWT KKLYPYSVSD YTMKNVQFLV
410 420 430 440 450
TSFLTVVLFL RLVRSDPGCL KTDDSLTSIQ ETIKQLIDLG KFDRENFCVE
460 470 480 490 500
TLERKPLRSK YSFFSGALVA RYDHYCPWIY NDVGLKNHKL FVFFAVTVQY
510 520 530 540 550
HMFLFMWLCL AYFKKTNYIY EQVEEYARCA LLKNETLCKG SNYDPSTFFL
560 570 580 590 600
FIWISVNFIW LGAMLIVQFF QILKGITTPE LFILIKEEHK AKFINLIPFE
610 620 630 640 650
NSIYTSESKG VEDSDMIPEG PSATTITHTI SIDGLEPRNR RRAILSACFS
660 670 680 690 700
MMGINQWLVT IKEIVGITHI LHGQVPQQHH SSLLRSFLVT NHWKTNLTDF
710 720 730 740
WLNSDVTAPL WQRFFYSSDT SKAMLGGTEV DYYELYEYPA REGEVLRPN
Length:749
Mass (Da):85,236
Last modified:November 1, 1996 - v1
Checksum:i887F5A02B9EE030A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87331 Genomic DNA. Translation: CAA60751.1.
Z74942 Genomic DNA. Translation: CAA99224.1.
BK006948 Genomic DNA. Translation: DAA10816.1.
PIRiS62171.
RefSeqiNP_014677.1. NM_001183453.1.

Genome annotation databases

EnsemblFungiiYOR034C; YOR034C; YOR034C.
GeneIDi854199.
KEGGisce:YOR034C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87331 Genomic DNA. Translation: CAA60751.1.
Z74942 Genomic DNA. Translation: CAA99224.1.
BK006948 Genomic DNA. Translation: DAA10816.1.
PIRiS62171.
RefSeqiNP_014677.1. NM_001183453.1.

3D structure databases

ProteinModelPortaliQ12013.
SMRiQ12013. Positions 17-264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34436. 20 interactions.
DIPiDIP-1882N.
IntActiQ12013. 6 interactions.
MINTiMINT-395171.

PTM databases

iPTMnetiQ12013.

Proteomic databases

MaxQBiQ12013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR034C; YOR034C; YOR034C.
GeneIDi854199.
KEGGisce:YOR034C.

Organism-specific databases

EuPathDBiFungiDB:YOR034C.
SGDiS000005560. AKR2.

Phylogenomic databases

GeneTreeiENSGT00530000063074.
HOGENOMiHOG000033883.
InParanoidiQ12013.
KOiK20032.
OMAiCIMAVIN.
OrthoDBiEOG092C216R.

Enzyme and pathway databases

BioCyciYEAST:G3O-33580-MONOMER.

Miscellaneous databases

PROiQ12013.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF01529. zf-DHHC. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS50216. DHHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKR2_YEAST
AccessioniPrimary (citable) accession number: Q12013
Secondary accession number(s): D6W2A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 815 molecules/cell in log phase SD medium.1 Publication

Caution

It is uncertain whether Met-1 or Met-4 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.