Reviewed, UniProtKB/Swiss-Prot Q12008 (PMG2_YEAST)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate mutase 2
      Short name=PGAM 2
    EC=5.4.2.1
Alternative name(s):
    Phosphoglyceromutase 2
    MPGM 2
    BPG-dependent PGAM 2

Gene names

Name:	GPM2
Ordered Locus Names:	YDL021W
ORF Names:	D2835

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Could be non-functional.
Catalytic activity	2-phospho-D-glycerate = 3-phospho-D-glycerate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 2020 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	phosphoglycerate mutase activity Ref.3 Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P36036	1	EBI-2063839,EBI-27015
HUG1	Q6Q5K6	1	EBI-2063839,EBI-392766
RNR1	P21524	1	EBI-2063839,EBI-15234
RNR2	P09938	1	EBI-2063839,EBI-15240
UBA1	P22515	1	EBI-2063839,EBI-19703

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Phosphoglycerate mutase 2		PRO_0000179840
Sites
	Active site	17	1	Tele-phosphohistidine intermediate By similarity
	Active site	242	1	By similarity
	Site	73	1	Interaction with carboxyl group of phosphoglycerates By similarity
Amino acid modifications
	Modified residue	74	1	Phosphothreonine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q12008-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 1E53ECAAF06C932C	FASTA	311	36,073
	10 20 30 40 50 60 MTASTPSNVM TLFLLRHGQS ELNHENIFCG WIDAKLTEKG KEQARHSAEL IEQYCKANNL 70 80 90 100 110 120 RLPQIGYTSR LIRTQQTIET MCEEFKLKPQ LQVVYDFNKI KLGDEFGSDD KDNMKIPILQ 130 140 150 160 170 180 TWRLNERHYG SWQGQRKPNV LKEYGKDKYM FIRRDYEGKP PPVDLDREMI QQENEKGSST 190 200 210 220 230 240 GYEFKEPNRQ IKYELECSNH DIVLPDSESL REVVYRLNPF LQNVILKLAN QYDESSCLIV 250 260 270 280 290 300 GHGSSVRSLL KILEGISDDD IKNVDIPNGI PLVVELDKNN GLKFIRKFYL DPESAKINAE 310 KVRNEGFIKN P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Investigation of two yeast genes encoding putative isoenzymes of phosphoglycerate mutase." Heinisch J.J., Mueller S., Schlueter E., Jacoby J., Rodicio R. Yeast 14:203-213(1998) [PubMed: 9544241] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed: 11502169] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-74, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z48432 Genomic DNA. Translation: CAA88338.1. Z74069 Genomic DNA. Translation: CAA98580.1. AY692876 Genomic DNA. Translation: AAT92895.1.
PIR	S52498.
RefSeq	NP_010263.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q12008. 7 interactions.
STRING	Q12008.
Proteomic databases
PeptideAtlas	Q12008.
PRIDE	Q12008.
Genome annotation databases
Ensembl	YDL021W; YDL021W; YDL021W; Saccharomyces cerevisiae. [Genome view]
GeneID	851541.
KEGG	sce:YDL021W.
NMPDR	fig|4932.3.peg.1005.
Organism-specific databases
CYGD	YDL021w.
SGD	S000002179. GPM2.
Phylogenomic databases
HOGENOM	Q12008.
OrthoDB	EOG9M3BS9
Enzyme and pathway databases
BRENDA	5.4.2.1. 250.
Gene expression databases
ArrayExpress	Q12008.
Genevestigator	Q12008.
GermOnline	YDL021W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001345. PG/BPGM_mutase_AS. IPR013078. PG_mutase. IPR005952. Phosphogly_mut1. [Graphical view]
PANTHER	PTHR11931. Phosphogly_mut1. 1 hit.
Pfam	PF00300. PGAM. 1 hit. [Graphical view]
SMART	SM00855. PGAM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01258. pgm_1. 1 hit.
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968946.

Entry information

Entry name

PMG2_YEAST

Accession

Primary (citable) accession number: Q12008

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)