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Protein

Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

ALG6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc2-PP-Dol.1 Publication

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: SGD
  • transferase activity, transferring hexosyl groups Source: SGD

GO - Biological processi

  • aerobic respiration Source: SGD
  • oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  • protein glycosylation Source: SGD
  • protein N-linked glycosylation Source: GO_Central

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YOR002W-MONOMER
YEAST:YOR002W-MONOMER
BRENDAi2.4.1.267 984
ReactomeiR-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT57 Glycosyltransferase Family 57

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.267)
Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:ALG6
Ordered Locus Names:YOR002W
ORF Names:UNA544
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YOR002W
SGDiS000005528 ALG6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 35CytoplasmicSequence analysisAdd BLAST35
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Topological domaini57 – 104LumenalSequence analysisAdd BLAST48
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Topological domaini126 – 145CytoplasmicSequence analysisAdd BLAST20
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Topological domaini167 – 171LumenalSequence analysis5
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Topological domaini193 – 223CytoplasmicSequence analysisAdd BLAST31
Transmembranei224 – 244HelicalSequence analysisAdd BLAST21
Topological domaini245 – 263LumenalSequence analysisAdd BLAST19
Transmembranei264 – 284HelicalSequence analysisAdd BLAST21
Topological domaini285 – 332CytoplasmicSequence analysisAdd BLAST48
Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
Topological domaini354 – 356LumenalSequence analysis3
Transmembranei357 – 377HelicalSequence analysisAdd BLAST21
Topological domaini378 – 398CytoplasmicSequence analysisAdd BLAST21
Transmembranei399 – 419HelicalSequence analysisAdd BLAST21
Topological domaini420 – 425LumenalSequence analysis6
Transmembranei426 – 446HelicalSequence analysisAdd BLAST21
Topological domaini447 – 481CytoplasmicSequence analysisAdd BLAST35
Transmembranei482 – 502HelicalSequence analysisAdd BLAST21
Topological domaini503 – 508LumenalSequence analysis6
Transmembranei509 – 529HelicalSequence analysisAdd BLAST21
Topological domaini530 – 544CytoplasmicSequence analysisAdd BLAST15

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741611 – 544Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST544

Proteomic databases

PaxDbiQ12001
PRIDEiQ12001

Interactioni

Protein-protein interaction databases

BioGridi34405, 271 interactors
STRINGi4932.YOR002W

Structurei

3D structure databases

ProteinModelPortaliQ12001
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075073
HOGENOMiHOG000195048
InParanoidiQ12001
KOiK03848
OMAiFINPDWI
OrthoDBiEOG092C0OC7

Family and domain databases

InterProiView protein in InterPro
IPR004856 Glyco_trans_ALG6/ALG8
PANTHERiPTHR12413 PTHR12413, 1 hit
PfamiView protein in Pfam
PF03155 Alg6_Alg8, 1 hit

Sequencei

Sequence statusi: Complete.

Q12001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIGKRLLVN KPAEESFYAS PMYDFLYPFR PVGNQWLPEY IIFVCAVILR
60 70 80 90 100
CTIGLGPYSG KGSPPLYGDF EAQRHWMEIT QHLPLSKWYW YDLQYWGLDY
110 120 130 140 150
PPLTAFHSYL LGLIGSFFNP SWFALEKSRG FESPDNGLKT YMRSTVIISD
160 170 180 190 200
ILFYFPAVIY FTKWLGRYRN QSPIGQSIAA SAILFQPSLM LIDHGHFQYN
210 220 230 240 250
SVMLGLTAYA INNLLDEYYA MAAVCFVLSI CFKQMALYYA PIFFAYLLSR
260 270 280 290 300
SLLFPKFNIA RLTVIAFATL ATFAIIFAPL YFLGGGLKNI HQCIHRIFPF
310 320 330 340 350
ARGIFEDKVA NFWCVTNVFV KYKERFTIQQ LQLYSLIATV IGFLPAMIMT
360 370 380 390 400
LLHPKKHLLP YVLIACSMSF FLFSFQVHEK TILIPLLPIT LLYSSTDWNV
410 420 430 440 450
LSLVSWINNV ALFTLWPLLK KDGLHLQYAV SFLLSNWLIG NFSFITPRFL
460 470 480 490 500
PKSLTPGPSI SSINSDYRRR SLLPYNVVWK SFIIGTYIAM GFYHFLDQFV
510 520 530 540
APPSKYPDLW VLLNCAVGFI CFSIFWLWSY YKIFTSGSKS MKDL
Length:544
Mass (Da):62,783
Last modified:November 1, 1997 - v1
Checksum:i64BFA11A1F6D02B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203M → V in AAT92914 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43491 Genomic DNA Translation: AAC49481.1
Z74910 Genomic DNA Translation: CAA99190.1
AY692895 Genomic DNA Translation: AAT92914.1
BK006948 Genomic DNA Translation: DAA10784.1
PIRiS61985
RefSeqiNP_014644.1, NM_001183421.1

Genome annotation databases

EnsemblFungiiYOR002W; YOR002W; YOR002W
GeneIDi854163
KEGGisce:YOR002W

Similar proteinsi

Entry informationi

Entry nameiALG6_YEAST
AccessioniPrimary (citable) accession number: Q12001
Secondary accession number(s): D6W268, E9P8Y7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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