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Protein

Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

ALG6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc(2)-PP-Dol.1 Publication

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: SGD
  • transferase activity, transferring hexosyl groups Source: SGD

GO - Biological processi

  • aerobic respiration Source: SGD
  • oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  • protein glycosylation Source: SGD
  • protein N-linked glycosylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YOR002W-MONOMER.
YEAST:YOR002W-MONOMER.
BRENDAi2.4.1.267. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.267)
Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:ALG6
Ordered Locus Names:YOR002W
ORF Names:UNA544
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR002W.
SGDiS000005528. ALG6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 35CytoplasmicSequence analysisAdd BLAST35
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Topological domaini57 – 104LumenalSequence analysisAdd BLAST48
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Topological domaini126 – 145CytoplasmicSequence analysisAdd BLAST20
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Topological domaini167 – 171LumenalSequence analysis5
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Topological domaini193 – 223CytoplasmicSequence analysisAdd BLAST31
Transmembranei224 – 244HelicalSequence analysisAdd BLAST21
Topological domaini245 – 263LumenalSequence analysisAdd BLAST19
Transmembranei264 – 284HelicalSequence analysisAdd BLAST21
Topological domaini285 – 332CytoplasmicSequence analysisAdd BLAST48
Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
Topological domaini354 – 356LumenalSequence analysis3
Transmembranei357 – 377HelicalSequence analysisAdd BLAST21
Topological domaini378 – 398CytoplasmicSequence analysisAdd BLAST21
Transmembranei399 – 419HelicalSequence analysisAdd BLAST21
Topological domaini420 – 425LumenalSequence analysis6
Transmembranei426 – 446HelicalSequence analysisAdd BLAST21
Topological domaini447 – 481CytoplasmicSequence analysisAdd BLAST35
Transmembranei482 – 502HelicalSequence analysisAdd BLAST21
Topological domaini503 – 508LumenalSequence analysis6
Transmembranei509 – 529HelicalSequence analysisAdd BLAST21
Topological domaini530 – 544CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: GO_Central
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741611 – 544Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST544

Proteomic databases

PRIDEiQ12001.

Interactioni

Protein-protein interaction databases

BioGridi34405. 171 interactors.
MINTiMINT-4502342.

Structurei

3D structure databases

ProteinModelPortaliQ12001.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075073.
HOGENOMiHOG000195048.
InParanoidiQ12001.
KOiK03848.
OMAiTTMAFFI.
OrthoDBiEOG092C0OC7.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 2 hits.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIGKRLLVN KPAEESFYAS PMYDFLYPFR PVGNQWLPEY IIFVCAVILR
60 70 80 90 100
CTIGLGPYSG KGSPPLYGDF EAQRHWMEIT QHLPLSKWYW YDLQYWGLDY
110 120 130 140 150
PPLTAFHSYL LGLIGSFFNP SWFALEKSRG FESPDNGLKT YMRSTVIISD
160 170 180 190 200
ILFYFPAVIY FTKWLGRYRN QSPIGQSIAA SAILFQPSLM LIDHGHFQYN
210 220 230 240 250
SVMLGLTAYA INNLLDEYYA MAAVCFVLSI CFKQMALYYA PIFFAYLLSR
260 270 280 290 300
SLLFPKFNIA RLTVIAFATL ATFAIIFAPL YFLGGGLKNI HQCIHRIFPF
310 320 330 340 350
ARGIFEDKVA NFWCVTNVFV KYKERFTIQQ LQLYSLIATV IGFLPAMIMT
360 370 380 390 400
LLHPKKHLLP YVLIACSMSF FLFSFQVHEK TILIPLLPIT LLYSSTDWNV
410 420 430 440 450
LSLVSWINNV ALFTLWPLLK KDGLHLQYAV SFLLSNWLIG NFSFITPRFL
460 470 480 490 500
PKSLTPGPSI SSINSDYRRR SLLPYNVVWK SFIIGTYIAM GFYHFLDQFV
510 520 530 540
APPSKYPDLW VLLNCAVGFI CFSIFWLWSY YKIFTSGSKS MKDL
Length:544
Mass (Da):62,783
Last modified:November 1, 1997 - v1
Checksum:i64BFA11A1F6D02B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203M → V in AAT92914 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43491 Genomic DNA. Translation: AAC49481.1.
Z74910 Genomic DNA. Translation: CAA99190.1.
AY692895 Genomic DNA. Translation: AAT92914.1.
BK006948 Genomic DNA. Translation: DAA10784.1.
PIRiS61985.
RefSeqiNP_014644.1. NM_001183421.1.

Genome annotation databases

EnsemblFungiiYOR002W; YOR002W; YOR002W.
GeneIDi854163.
KEGGisce:YOR002W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43491 Genomic DNA. Translation: AAC49481.1.
Z74910 Genomic DNA. Translation: CAA99190.1.
AY692895 Genomic DNA. Translation: AAT92914.1.
BK006948 Genomic DNA. Translation: DAA10784.1.
PIRiS61985.
RefSeqiNP_014644.1. NM_001183421.1.

3D structure databases

ProteinModelPortaliQ12001.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34405. 171 interactors.
MINTiMINT-4502342.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Proteomic databases

PRIDEiQ12001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR002W; YOR002W; YOR002W.
GeneIDi854163.
KEGGisce:YOR002W.

Organism-specific databases

EuPathDBiFungiDB:YOR002W.
SGDiS000005528. ALG6.

Phylogenomic databases

GeneTreeiENSGT00550000075073.
HOGENOMiHOG000195048.
InParanoidiQ12001.
KOiK03848.
OMAiTTMAFFI.
OrthoDBiEOG092C0OC7.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:YOR002W-MONOMER.
YEAST:YOR002W-MONOMER.
BRENDAi2.4.1.267. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiQ12001.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 2 hits.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG6_YEAST
AccessioniPrimary (citable) accession number: Q12001
Secondary accession number(s): D6W268, E9P8Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.