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Protein

Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

ALG6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc(2)-PP-Dol.1 Publication

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: SGD
  • transferase activity, transferring hexosyl groups Source: SGD

GO - Biological processi

  • aerobic respiration Source: SGD
  • oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  • protein glycosylation Source: SGD
  • protein N-linked glycosylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-7191.
YEAST:YOR002W-MONOMER.
BRENDAi2.4.1.267. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.267)
Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:ALG6
Ordered Locus Names:YOR002W
ORF Names:UNA544
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR002W.
SGDiS000005528. ALG6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535CytoplasmicSequence analysisAdd
BLAST
Transmembranei36 – 5621HelicalSequence analysisAdd
BLAST
Topological domaini57 – 10448LumenalSequence analysisAdd
BLAST
Transmembranei105 – 12521HelicalSequence analysisAdd
BLAST
Topological domaini126 – 14520CytoplasmicSequence analysisAdd
BLAST
Transmembranei146 – 16621HelicalSequence analysisAdd
BLAST
Topological domaini167 – 1715LumenalSequence analysis
Transmembranei172 – 19221HelicalSequence analysisAdd
BLAST
Topological domaini193 – 22331CytoplasmicSequence analysisAdd
BLAST
Transmembranei224 – 24421HelicalSequence analysisAdd
BLAST
Topological domaini245 – 26319LumenalSequence analysisAdd
BLAST
Transmembranei264 – 28421HelicalSequence analysisAdd
BLAST
Topological domaini285 – 33248CytoplasmicSequence analysisAdd
BLAST
Transmembranei333 – 35321HelicalSequence analysisAdd
BLAST
Topological domaini354 – 3563LumenalSequence analysis
Transmembranei357 – 37721HelicalSequence analysisAdd
BLAST
Topological domaini378 – 39821CytoplasmicSequence analysisAdd
BLAST
Transmembranei399 – 41921HelicalSequence analysisAdd
BLAST
Topological domaini420 – 4256LumenalSequence analysis
Transmembranei426 – 44621HelicalSequence analysisAdd
BLAST
Topological domaini447 – 48135CytoplasmicSequence analysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence analysisAdd
BLAST
Topological domaini503 – 5086LumenalSequence analysis
Transmembranei509 – 52921HelicalSequence analysisAdd
BLAST
Topological domaini530 – 54415CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: GO_Central
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 544544Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferasePRO_0000174161Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi34405. 171 interactions.
MINTiMINT-4502342.

Structurei

3D structure databases

ProteinModelPortaliQ12001.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075073.
HOGENOMiHOG000195048.
InParanoidiQ12001.
KOiK03848.
OMAiTTMAFFI.
OrthoDBiEOG092C0OC7.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 2 hits.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIGKRLLVN KPAEESFYAS PMYDFLYPFR PVGNQWLPEY IIFVCAVILR
60 70 80 90 100
CTIGLGPYSG KGSPPLYGDF EAQRHWMEIT QHLPLSKWYW YDLQYWGLDY
110 120 130 140 150
PPLTAFHSYL LGLIGSFFNP SWFALEKSRG FESPDNGLKT YMRSTVIISD
160 170 180 190 200
ILFYFPAVIY FTKWLGRYRN QSPIGQSIAA SAILFQPSLM LIDHGHFQYN
210 220 230 240 250
SVMLGLTAYA INNLLDEYYA MAAVCFVLSI CFKQMALYYA PIFFAYLLSR
260 270 280 290 300
SLLFPKFNIA RLTVIAFATL ATFAIIFAPL YFLGGGLKNI HQCIHRIFPF
310 320 330 340 350
ARGIFEDKVA NFWCVTNVFV KYKERFTIQQ LQLYSLIATV IGFLPAMIMT
360 370 380 390 400
LLHPKKHLLP YVLIACSMSF FLFSFQVHEK TILIPLLPIT LLYSSTDWNV
410 420 430 440 450
LSLVSWINNV ALFTLWPLLK KDGLHLQYAV SFLLSNWLIG NFSFITPRFL
460 470 480 490 500
PKSLTPGPSI SSINSDYRRR SLLPYNVVWK SFIIGTYIAM GFYHFLDQFV
510 520 530 540
APPSKYPDLW VLLNCAVGFI CFSIFWLWSY YKIFTSGSKS MKDL
Length:544
Mass (Da):62,783
Last modified:November 1, 1997 - v1
Checksum:i64BFA11A1F6D02B7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti203 – 2031M → V in AAT92914 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43491 Genomic DNA. Translation: AAC49481.1.
Z74910 Genomic DNA. Translation: CAA99190.1.
AY692895 Genomic DNA. Translation: AAT92914.1.
BK006948 Genomic DNA. Translation: DAA10784.1.
PIRiS61985.
RefSeqiNP_014644.1. NM_001183421.1.

Genome annotation databases

EnsemblFungiiYOR002W; YOR002W; YOR002W.
GeneIDi854163.
KEGGisce:YOR002W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43491 Genomic DNA. Translation: AAC49481.1.
Z74910 Genomic DNA. Translation: CAA99190.1.
AY692895 Genomic DNA. Translation: AAT92914.1.
BK006948 Genomic DNA. Translation: DAA10784.1.
PIRiS61985.
RefSeqiNP_014644.1. NM_001183421.1.

3D structure databases

ProteinModelPortaliQ12001.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34405. 171 interactions.
MINTiMINT-4502342.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR002W; YOR002W; YOR002W.
GeneIDi854163.
KEGGisce:YOR002W.

Organism-specific databases

EuPathDBiFungiDB:YOR002W.
SGDiS000005528. ALG6.

Phylogenomic databases

GeneTreeiENSGT00550000075073.
HOGENOMiHOG000195048.
InParanoidiQ12001.
KOiK03848.
OMAiTTMAFFI.
OrthoDBiEOG092C0OC7.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:MONOMER-7191.
YEAST:YOR002W-MONOMER.
BRENDAi2.4.1.267. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiQ12001.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 2 hits.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG6_YEAST
AccessioniPrimary (citable) accession number: Q12001
Secondary accession number(s): D6W268, E9P8Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.