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Q11VK7 (SYL_CYTH3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CHU_1287
OrganismCytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) [Complete proteome] [HAMAP]
Taxonomic identifier269798 [NCBI]
Taxonomic lineageBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeCytophaga

Protein attributes

Sequence length921 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 921921Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000074831

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif695 – 6995"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6981ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q11VK7 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: FEE623E5B6E57BFB

FASTA921104,655
        10         20         30         40         50         60 
MADYHFGEVE NKWQSKWNAE KTFKALENSS KPKYFVLDMF PYPSGSGLHV GHPLGYIASD 

        70         80         90        100        110        120 
IMARYKRIKG YNVLHPMGFD SFGLPAEQYA VQTGQHPAIT TEQNIARYIE QLNKIGFSFD 

       130        140        150        160        170        180 
WDREIRTSDP AYYKWTQWIF IQLFNHWYNK ASDKAEPITN LVKQFETAGN AGINAACDED 

       190        200        210        220        230        240 
AVTFTAADWK SYSEKQQSDT LLKYRLTYLS ETMVNWCPGL GTVLANEEVK DGLSERGGFP 

       250        260        270        280        290        300 
VERKKMKQWS MRITAYADRL LKGLDTIDWP EAMKEMQRYW IGKSLGAMLT FKVVDKDMEL 

       310        320        330        340        350        360 
TVFTTRIDTT FGVTYVSIAP EHEWIGALTS PEQKAAVEEY VTKAKNRSER DRMSDVKTVS 

       370        380        390        400        410        420 
GCFTGSYVTN PFNNEKIPVW IADYVLAGYG TGVVMAVPSS DERDYKFASF YKLPIISVQE 

       430        440        450        460        470        480 
GAHTDITKED FDPKAGTMIN SGFLNGLTVK QAIPEAIKFI EEKKIGFAKI NFKMRDSIFG 

       490        500        510        520        530        540 
RQRYWGEPIP VSYKNDIPYV LNESELPLAL PAIDEYKPTE TGEPPLARNK AFADKGYELS 

       550        560        570        580        590        600 
TMPGWAGSSW YFMRYMDPQN KTAVAAADKI NYWGQVDLYM GGAEHATGHL LYSRFWNKFL 

       610        620        630        640        650        660 
FDMGITPNDE PFAKLINQGM IQGVSKFAYR INGTNKFVSA GLKKEYDTTP IHVDVSIVHN 

       670        680        690        700        710        720 
DVLDTEAFKK WRPDFASAEF ILENNTYVCG SEVEKMSKSK FNVVNPDDIV NKYGADTLRM 

       730        740        750        760        770        780 
YEMFLGPLEQ SKPWNTNGIE GVYKFLNRFW RLFHDTAGNF AVSDAQPTAE ELKVLHKTLK 

       790        800        810        820        830        840 
RVEEDIERFS FNTPVSTFMI CVNELGSLKC NKRTILEPLT IALSPLAPHI AEELWSLLGH 

       850        860        870        880        890        900 
TTSVSTATYP AWDEKFLVES NHEYPISING KMRAKLNLPV DMPAAEVEQQ VLANEVVQKW 

       910        920 
LEGKAPKKII VIPNKIVNVV M 

« Hide

References

[1]"Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii."
Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P., Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R., Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B., McBride M.J.
Appl. Environ. Microbiol. 73:3536-3546(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33406 / NCIMB 9469.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000383 Genomic DNA. Translation: ABG58559.1.
RefSeqYP_677899.1. NC_008255.1.

3D structure databases

ProteinModelPortalQ11VK7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269798.CHU_1287.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG58559; ABG58559; CHU_1287.
GeneID4185986.
KEGGchu:CHU_1287.
PATRIC21593423. VBICytHut34013_1285.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAERYWMNV.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCHUT269798:GJ83-1285-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_CYTH3
AccessionPrimary (citable) accession number: Q11VK7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 22, 2006
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries