Q11VI2 (DAPA_CYTH3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	CHU_1312

Organism

Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) [Complete proteome] [HAMAP]

Taxonomic identifier

269798 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Cytophagia › Cytophagales › Cytophagaceae › Cytophaga

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000340943
Regions
	Region	50 – 51	2	Pyruvate binding By similarity
Sites
	Active site	163	1	Schiff-base intermediate with substrate By similarity
	Binding site	108	1	Pyruvate By similarity
	Site	135	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11VI2 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: A5DD03DD6F0F5A74	FASTA	297	32,243
	10 20 30 40 50 60 MHTFYGTGTA LITPFKKDHS VDYDAFKNLI EFNISSGVDY IVVNGTTAES ATTSKEEKAK 70 80 90 100 110 120 LLEVALDVNN GRVPVMYGIG GNDTAEVIQR IGSADFKGVD ALLSVCPYYN KPSQEGVFQH 130 140 150 160 170 180 YKAIAEKCPV PVLAYNVPGR TGINISAKTS IRLSEVKNIF GIKEASGDLV QALEIIKNTP 190 200 210 220 230 240 NEFLVISGDD LLAVPTISLG AVGAISVLSN TYPAKFSEMI KAAIQSRFQQ ATQILARFTE 250 260 270 280 290 MNPLMYEEGN PVGVKSLLEL MGICSSEVRL PLVKASGELI HKIEQAHVKI TREYANH

References

[1]

"Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii." Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P., Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R., Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B., McBride M.J. Appl. Environ. Microbiol. 73:3536-3546(2007) [PubMed: 17400776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33406 / NCIMB 9469.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000383 Genomic DNA. Translation: ABG58584.1.
RefSeq	YP_677924.1. NC_008255.1.
3D structure databases
ProteinModelPortal	Q11VI2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q11VI2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4186122.
GenomeReviews	Gene locus CHU_1312 in contig CP000383_GR.
KEGG	chu:CHU_1312.
NMPDR	fig|269798.12.peg.1251.
PATRIC	21593467. VBICytHut34013_1307.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	CEMEDSN.
ProtClustDB	CLSK2530613.
Enzyme and pathway databases
BioCyc	CHUT269798:CHU_1312-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_CYTH3

Accession

Primary (citable) accession number: Q11VI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program