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Q11S94

- BIOB_CYTH3

UniProt

Q11S94 - BIOB_CYTH3

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Protein
Biotin synthase
Gene
bioB, CHU_2466
Organism
Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi58 – 581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi98 – 981Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi130 – 1301Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi190 – 1901Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi262 – 2621Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCHUT269798:GJ83-2464-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:CHU_2466
OrganismiCytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Taxonomic identifieri269798 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeCytophaga
ProteomesiUP000001822: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Biotin synthaseUniRule annotation
PRO_0000381341Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi269798.CHU_2466.

Structurei

3D structure databases

ProteinModelPortaliQ11S94.
SMRiQ11S94. Positions 5-317.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRDFHGMA.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q11S94-1 [UniParc]FASTAAdd to Basket

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MTEIRNNWTK EEISAIYNSP ILDLMYRGAT VHREFHDPQE VQVCTLLSIK    50
TGGCPEDCSY CPQAARYHTD VKVEKLMDVK DVLNSALEAK ESGSTRFCMG 100
AAWREVRDNK DFDKVIDMVK GVSTMGMEVC CTLGMLTPEQ ADKLKDAGLY 150
AYNHNLDTSA EHYDKVITTR TYDDRLETLD NVRNAKISVC SGGIIGMGES 200
HGDRVGMLHT LANMVEHPES VPVNALVPVE GTPLEDQPRV SVWEMVRMIA 250
TARIIMPKAM VRLSAGRVRM NTEEQALCFL AGANSIFAGD KLLTTPNPEV 300
NADKEMFQVL NLKPRQSFKN GDAPKIKFEQ IPSALVK 337
Length:337
Mass (Da):37,532
Last modified:August 22, 2006 - v1
Checksum:iD7B65D023605991D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000383 Genomic DNA. Translation: ABG59720.1.
RefSeqiWP_011585834.1. NC_008255.1.
YP_679062.1. NC_008255.1.

Genome annotation databases

EnsemblBacteriaiABG59720; ABG59720; CHU_2466.
GeneIDi4184415.
KEGGichu:CHU_2466.
PATRICi21595811. VBICytHut34013_2464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000383 Genomic DNA. Translation: ABG59720.1 .
RefSeqi WP_011585834.1. NC_008255.1.
YP_679062.1. NC_008255.1.

3D structure databases

ProteinModelPortali Q11S94.
SMRi Q11S94. Positions 5-317.
ModBasei Search...

Protein-protein interaction databases

STRINGi 269798.CHU_2466.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG59720 ; ABG59720 ; CHU_2466 .
GeneIDi 4184415.
KEGGi chu:CHU_2466.
PATRICi 21595811. VBICytHut34013_2464.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RDFHGMA.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CHUT269798:GJ83-2464-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33406 / NCIMB 9469.

Entry informationi

Entry nameiBIOB_CYTH3
AccessioniPrimary (citable) accession number: Q11S94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 22, 2006
Last modified: September 3, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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