Reviewed, UniProtKB/Swiss-Prot Q11RG9 (MURE_CYTH3)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	CHU_2745

Organism

Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) [Complete proteome] [HAMAP]

Taxonomic identifier

269798 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Sphingobacteria › Sphingobacteriales › Flexibacteraceae › Cytophaga

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity HAMAP MF_00208.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000012350
Regions
	Nucleotide binding	112 – 118	7	ATP Potential
	Region	154 – 155	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	402 – 405	4	Meso-diaminopimelate binding By similarity
	Motif	402 – 405	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	30	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	181	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	187	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	189	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	378	1	Meso-diaminopimelate By similarity
	Binding site	455	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	459	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	221	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11RG9-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 2FB7EB012E12EAF1	FASTA	486	53,279
	10 20 30 40 50 60 MQIKDLIYKV SLISVSGRTD VDVTAICFDS RKVEKGSMFI AVRGVSSDGH SFIADVIQKG 70 80 90 100 110 120 ATAVVCEELP EIESTADCTI IQVKDSAEAL GMIASNFYDS PSSKLKLVGV TGTNGKTTTV 130 140 150 160 170 180 TLLYRLFRKL GYKTGLLSTV ENIIEDKVVQ ATHTTPDAIS LNKLLADMVK AGCTHCFMEV 190 200 210 220 230 240 SSHAAVQRRI AGLQFAGGLF SNITHDHLDY HKTFDEYIKA KKLFFDGLPN DSFALINSDD 250 260 270 280 290 300 KRGRVMIQNT RAKTYTYSLL ALADFKGKLI STTMQGLEMD VDGIQAWFRL IGNFNAYNLL 310 320 330 340 350 360 AVYATAVLLG EDKEEVLMQL STIEAANGRF EQQISATRIT VIVDYAHTPD ALKNVLETIT 370 380 390 400 410 420 ELKGANKIIT VVGCGGNRDA AKRPVMADIA CQFSDHVVLT SDNPRNEEPQ AILTEMEKGV 430 440 450 460 470 480 RIVDKKKVLS VLDRKEAIKV ACTLASTGDI ILVAGKGHET YQEIKGVKYP FDDRLIIKEL LDTLGK

References

[1]

"Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii." Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P., Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R., Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B., McBride M.J. Appl. Environ. Microbiol. 73:3536-3546(2007) [PubMed: 17400776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000383 Genomic DNA. Translation: ABG59995.1.
RefSeq	YP_679337.1.
3D structure databases
SMR	Q11RG9. Positions 2-486.
ModBase	Search...
Protein-protein interaction databases
STRING	Q11RG9.
Genome annotation databases
GeneID	4185335.
GenomeReviews	Gene locus CHU_2745 in contig CP000383_GR.
KEGG	chu:CHU_2745.
NMPDR	fig|269798.12.peg.2634.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0769.
HOGENOM	HBG602753.
OMA	HTPDGIE.
Enzyme and pathway databases
BioCyc	CHUT269798:CHU_2745-MONOMER.
Family and domain databases
HAMAP	MF_00208. MurE. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_CYTH3

Accession

Primary (citable) accession number: Q11RG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)