UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase - Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

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Reviewed, UniProtKB/Swiss-Prot Q11RG9 (MURE_CYTH3)

Last modified June 16, 2009. Version 27. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	CHU_2745

Organism

Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) [Complete proteome] [HAMAP]

Taxonomic identifier

269798 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Sphingobacteria › Sphingobacteriales › Flexibacteraceae › Cytophaga

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Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg²⁺ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

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Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 486

486

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208

PRO_1000012350

Regions

Nucleotide binding

112 – 118

ATP Potential

Region

154 – 155

UDP-MurNAc-L-Ala-D-Glu binding By similarity

Region

402 – 405

Meso-diaminopimelate binding By similarity

Motif

402 – 405

Meso-diaminopimelate recognition motif HAMAP MF_00208

Sites

Binding site

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

181

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

187

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

189

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

378

Meso-diaminopimelate By similarity

Binding site

455

Meso-diaminopimelate; via carbonyl oxygen By similarity

Binding site

459

Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue

221

N6-carboxylysine By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q11RG9-1 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 2FB7EB012E12EAF1
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FASTA

486

53,279

        10         20         30         40         50         60 
MQIKDLIYKV SLISVSGRTD VDVTAICFDS RKVEKGSMFI AVRGVSSDGH SFIADVIQKG 

        70         80         90        100        110        120 
ATAVVCEELP EIESTADCTI IQVKDSAEAL GMIASNFYDS PSSKLKLVGV TGTNGKTTTV 

       130        140        150        160        170        180 
TLLYRLFRKL GYKTGLLSTV ENIIEDKVVQ ATHTTPDAIS LNKLLADMVK AGCTHCFMEV 

       190        200        210        220        230        240 
SSHAAVQRRI AGLQFAGGLF SNITHDHLDY HKTFDEYIKA KKLFFDGLPN DSFALINSDD 

       250        260        270        280        290        300 
KRGRVMIQNT RAKTYTYSLL ALADFKGKLI STTMQGLEMD VDGIQAWFRL IGNFNAYNLL 

       310        320        330        340        350        360 
AVYATAVLLG EDKEEVLMQL STIEAANGRF EQQISATRIT VIVDYAHTPD ALKNVLETIT 

       370        380        390        400        410        420 
ELKGANKIIT VVGCGGNRDA AKRPVMADIA CQFSDHVVLT SDNPRNEEPQ AILTEMEKGV 

       430        440        450        460        470        480 
RIVDKKKVLS VLDRKEAIKV ACTLASTGDI ILVAGKGHET YQEIKGVKYP FDDRLIIKEL 


LDTLGK

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References

[1]

"Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii."
Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P., Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R., Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B., McBride M.J.
Appl. Environ. Microbiol. 73:3536-3546(2007) [PubMed: 17400776] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases
	CP000383 Genomic DNA. Translation: ABG59995.1.
RefSeq	YP_679337.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4185335.
GenomeReviews	Gene locus CHU_2745 in contig CP000383_GR.
KEGG	chu:CHU_2745.
NMPDR	fig\|269798.12.peg.2634.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q11RG9.
OMA	Q11RG9. HTPDGIE.
Enzyme and pathway databases
BioCyc	CHUT269798:CHU_2745-MON.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

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Entry information

Entry name

MURE_CYTH3

Accession

Primary (citable) accession number: Q11RG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents