Reviewed, UniProtKB/Swiss-Prot Q11RE2 (K6PF_CYTH3)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase
      Short name=Phosphofructokinase
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	CHU_2773

Organism

Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) [Complete proteome] [HAMAP]

Taxonomic identifier

269798 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Sphingobacteria › Sphingobacteriales › Flexibacteraceae › Cytophaga

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	6-phosphofructokinase HAMAP MF_00339		PRO_1000059756
Regions
	Nucleotide binding	21 – 25	5	ATP By similarity
	Nucleotide binding	155 – 159	5	ATP By similarity
	Nucleotide binding	172 – 188	17	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Metal binding	186	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	188	1	Magnesium By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	247	1	Substrate By similarity
	Binding site	253	1	Substrate By similarity
	Binding site	256	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11RE2-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: A3897A05791AB459	FASTA	328	35,478
	10 20 30 40 50 60 MKKIAVYTSG GDAPGMNACI RAAVRAGHYH NLEVYGIVRG YDGMINGNFI ELDNRAVSNI 70 80 90 100 110 120 IQKGGTILKS ARSEEFRSPE GRRKAFDQIQ KFGIDGLVAI GGNGTFTGAE LFYNEYQIPC 130 140 150 160 170 180 VGAPGTIDND LYGSDFTIGY DTAVNTCLAA IDKIRDTADA HERVFFIEVM GRDTGYIAVR 190 200 210 220 230 240 SAISGGAEMA IIPEFPYDKN VVINRLKHGT SDTKASHIII VAEGHQEGRA SMIARDIKEA 250 260 270 280 290 300 LPQLDCRVTT LGHVQRGGGP SAADRVLASR LGLATVEGLL EGRANVMAGI IDHKVVYTPF 310 320 IDTITKRKPI NEDLLSLAEK LNGRSYKV

References

[1]

"Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii." Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P., Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R., Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B., McBride M.J. Appl. Environ. Microbiol. 73:3536-3546(2007) [PubMed: 17400776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000383 Genomic DNA. Translation: ABG60022.1.
RefSeq	YP_679364.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4186262.
GenomeReviews	Gene locus CHU_2773 in contig CP000383_GR.
KEGG	chu:CHU_2773.
NMPDR	fig|269798.12.peg.2660.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q11RE2.
OMA	Q11RE2. CLGVDTA.
Enzyme and pathway databases
BioCyc	CHUT269798:CHU_2773-MON.
Family and domain databases
HAMAP	MF_00339. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_CYTH3

Accession

Primary (citable) accession number: Q11RE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)