Q11QU6 (BIOD_CYTH3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	CHU_2977

Organism

Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) [Complete proteome] [HAMAP]

Taxonomic identifier

269798 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Cytophagia › Cytophagales › Cytophagaceae › Cytophaga

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000302504
Regions
	Nucleotide binding	99 – 102	4	ATP By similarity
Sites
	Metal binding	12	1	Magnesium 1 By similarity
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	99	1	Magnesium 2 By similarity
	Binding site	206	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11QU6 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 5C2D2F081B2D480C	FASTA	206	22,683
	10 20 30 40 50 60 MKKYFITAIS TDSGKTLVSA ILTNALQADY WKPVQAGLEE TDSNTIRTLL HSGHSIIHPE 70 80 90 100 110 120 AYALKQAVSP HAAARNEGVE IVLAAIQLPD TNGNDLIIEG AGGVLVPLND TDVVADLITQ 130 140 150 160 170 180 FDAEVILVSN LYLGSINHTL LTAQELKRRN IRVKGIVFNG PRNEESERII LKHTGYKVLL 190 200 HVSPEEKITK EVVTAYAVKL FDNWYE

References

[1]

"Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii." Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P., Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R., Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B., McBride M.J. Appl. Environ. Microbiol. 73:3536-3546(2007) [PubMed: 17400776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33406 / NCIMB 9469.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000383 Genomic DNA. Translation: ABG60218.1.
RefSeq	YP_679560.1. NC_008255.1.
3D structure databases
ProteinModelPortal	Q11QU6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q11QU6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4185086.
GenomeReviews	Gene locus CHU_2977 in contig CP000383_GR.
KEGG	chu:CHU_2977.
NMPDR	fig|269798.12.peg.2842.
PATRIC	21596815. VBICytHut34013_2959.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	WKPIQSG.
Enzyme and pathway databases
BioCyc	CHUT269798:CHU_2977-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CYTH3

Accession

Primary (citable) accession number: Q11QU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program