Q11QQ3 (MDH_CYTH3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	CHU_3020

Organism

Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) [Complete proteome] [HAMAP]

Taxonomic identifier

269798 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Cytophagia › Cytophagales › Cytophagaceae › Cytophaga › Cytophaga hutchinsonii

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_1000026470
Regions
	Nucleotide binding	7 – 12	6	NAD By similarity
	Nucleotide binding	118 – 120	3	NAD By similarity
Sites
	Active site	175	1	Proton acceptor By similarity
	Binding site	32	1	NAD By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	95	1	NAD By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	151	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11QQ3 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: B0C3C5CA64561F02	FASTA	312	33,351
	10 20 30 40 50 60 MKVTVVGAGN VGATCADVLA YREIVNEVIL LDIKEGVAEG KALDIWQKAP ITQYDTKTTG 70 80 90 100 110 120 VTNDYSKTAN SDVVVITSGL PRKPGMTRDD LISTNAGIVR AVTESVVKYS PNAIIIVVSN 130 140 150 160 170 180 PLDVMTYCAH ITSKLPRNKV IGMAGVLDTA RYRAFLADEI GCSPKEIQGM LLGGHGDTMV 190 200 210 220 230 240 PLPRYTTVGG IPVTELVEAD KLNAIIERTK NGGGELVKLM GTSAWYAPGA AAAQMVEAIV 250 260 270 280 290 300 KDQKKIIPVC IKLEGEYGID DCYLGVPAVI GKNGIEKVIE LKLNAEEKAL METSRKHVKE 310 VMNVLDGLPQ QA

References

[1]

"Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii." Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P., Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R., Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B., McBride M.J. Appl. Environ. Microbiol. 73:3536-3546(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33406 / NCIMB 9469.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000383 Genomic DNA. Translation: ABG60261.1.
RefSeq	YP_679603.1. NC_008255.1.
3D structure databases
ProteinModelPortal	Q11QQ3.
SMR	Q11QQ3. Positions 1-303.
ModBase	Search...
Protein-protein interaction databases
STRING	269798.CHU_3020.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABG60261; ABG60261; CHU_3020.
GeneID	4184953.
KEGG	chu:CHU_3020.
PATRIC	21596907. VBICytHut34013_3003.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	DAMTYVM.
ProtClustDB	CLSK2530842.
Enzyme and pathway databases
BioCyc	CHUT269798:GJ83-3014-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_CYTH3

Accession

Primary (citable) accession number: Q11QQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program