Q11Q97 (SYN_CYTH3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	CHU_3176

Organism

Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) [Complete proteome] [HAMAP]

Taxonomic identifier

269798 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Cytophagia › Cytophagales › Cytophagaceae › Cytophaga

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm By similarity HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Asparagine--tRNA ligase HAMAP MF_00534		PRO_1000211900

Sequences

	Sequence		Length	Mass (Da)
	Q11Q97 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 872F10D811959A63	FASTA	464	52,658
	10 20 30 40 50 60 MKRIKIKELL SNSSQYIDQQ VVLKGWVRTK RGNKHVAFVA LNDGSTIHTI QIVLDVAKFN 70 80 90 100 110 120 EEQLKDVTTG ACISISGKVV TSQGAGQSIE VQGDTLEVIG VADPETYPLQ KKGHSLEFLR 130 140 150 160 170 180 EIAHLRPRTN TFSCVLRLRH AMAFAVHSFF NEKGFVYVHT PIITGSDAEG AGAMFQVTTL 190 200 210 220 230 240 DLKNPSKTKE GEVDFTKDFF GKATNLTVSG QLEGELAAMA LGEVYTFGPT FRAENSNTTR 250 260 270 280 290 300 HLAEFWMIEP EMAFYEIQDN MDLAEEFLKY LVKYALDNCI DDLNFLNEMY DKELIERLKS 310 320 330 340 350 360 VVETPFVRLN YTEAVDILIA TKQKFEYKVE WGIDLQSEHE RYLVEKHFKK PVILTNYPKA 370 380 390 400 410 420 IKAFYMKANE DGKTVRAMDV LFPGIGEIIG GSQREDNLEK LQERCKEVGI HENDIWWYLE 430 440 450 460 TRKFGSAPHS GFGLGFERLM LFVTGMGNIR DVIPFPRTPQ SAEF

References

[1]

"Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii." Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P., Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R., Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B., McBride M.J. Appl. Environ. Microbiol. 73:3536-3546(2007) [PubMed: 17400776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33406 / NCIMB 9469.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000383 Genomic DNA. Translation: ABG60416.1.
RefSeq	YP_679759.1. NC_008255.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q11Q97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4186102.
GenomeReviews	Gene locus CHU_3176 in contig CP000383_GR.
KEGG	chu:CHU_3176.
NMPDR	fig|269798.12.peg.3033.
PATRIC	21597231. VBICytHut34013_3161.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0017.
HOGENOM	HBG745843.
OMA	AIHRFFH.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	CHUT269798:CHU_3176-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_CYTH3

Accession

Primary (citable) accession number: Q11Q97

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program