Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

panB

Organism
Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. no protein annotated in this organism
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52MagnesiumUniRule annotation1
Metal bindingi91MagnesiumUniRule annotation1
Binding sitei91Alpha-ketoisovalerateUniRule annotation1
Binding sitei121Alpha-ketoisovalerateUniRule annotation1
Metal bindingi123MagnesiumUniRule annotation1
Active sitei190Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPantothenate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCHUT269798:G1G6N-3168-MONOMER
UniPathwayiUPA00028; UER00003

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotation (EC:2.1.2.11UniRule annotation)
Alternative name(s):
Ketopantoate hydroxymethyltransferaseUniRule annotation
Short name:
KPHMTUniRule annotation
Gene namesi
Name:panBUniRule annotation
Ordered Locus Names:CHU_3218
OrganismiCytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Taxonomic identifieri269798 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeCytophaga
Proteomesi
  • UP000001822 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002972541 – 2723-methyl-2-oxobutanoate hydroxymethyltransferaseAdd BLAST272

Proteomic databases

PRIDEiQ11Q55

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi269798.CHU_3218

Structurei

3D structure databases

ProteinModelPortaliQ11Q55
SMRiQ11Q55
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 53Alpha-ketoisovalerate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PanB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCG Bacteria
COG0413 LUCA
HOGENOMiHOG000078427
KOiK00606
OMAiGHIGLMP
OrthoDBiPOG091H02K8

Family and domain databases

CDDicd06557 KPHMT-like, 1 hit
HAMAPiMF_00156 PanB, 1 hit
InterProiView protein in InterPro
IPR003700 Pantoate_hydroxy_MeTrfase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR20881 PTHR20881, 1 hit
PfamiView protein in Pfam
PF02548 Pantoate_transf, 1 hit
PIRSFiPIRSF000388 Pantoate_hydroxy_MeTrfase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR00222 panB, 1 hit

Sequencei

Sequence statusi: Complete.

Q11Q55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTAKSDIKK VTTHQLQEMK NRGEKISMLT AYDYSMAKIL DAAGVDVILV
60 70 80 90 100
GDSASNVMAG HETTLPITLD QMIYHASSVI RAINRALVVV DLPFGSYQGN
110 120 130 140 150
SSEALRSAIR IMKESGAHAV KMEGGIEIKE SVLCIISAGI PVMGHLGLMP
160 170 180 190 200
QSIYKFGTYV VRAREEEEAN KLIADAKILE ESGCFGLVLE KIPASLSKTV
210 220 230 240 250
THQLQIPTIG IGAGPDVDGQ VLVTHDLLGI NNEFHPRFLR TYANLQATIS
260 270
TAVTSYIDDV KSKNFPNENE CY
Length:272
Mass (Da):29,541
Last modified:August 22, 2006 - v1
Checksum:i9A5FA9D189F1A76B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000383 Genomic DNA Translation: ABG60458.1
RefSeqiWP_011586568.1, NZ_FPJX01000011.1

Genome annotation databases

EnsemblBacteriaiABG60458; ABG60458; CHU_3218
KEGGichu:CHU_3218

Similar proteinsi

Entry informationi

Entry nameiPANB_CYTH3
AccessioniPrimary (citable) accession number: Q11Q55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: August 22, 2006
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health