Reviewed, UniProtKB/Swiss-Prot Q11PV4 (METE_CYTH3)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	CHU_3320

Organism

Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) [Complete proteome] [HAMAP]

Taxonomic identifier

269798 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Sphingobacteria › Sphingobacteriales › Flexibacteraceae › Cytophaga

Protein attributes

Sequence length	775 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 775	775	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097824
Sites
	Metal binding	658	1	Zinc By similarity
	Metal binding	660	1	Zinc By similarity
	Metal binding	743	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11PV4-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 21AAEDF406B850BA	FASTA	775	88,314
	10 20 30 40 50 60 MIAHNLGYPR IGDNRELKKA CEQYWSGAIT QQKLQDVAKA IRTNNWLTQK DAGLDLIPCN 70 80 90 100 110 120 DFSFYDQVLD MSFMLGLIPE RFSSLLQKKD LTELDIYFAM ARGYQKDGYD ITAMEMTKWF 130 140 150 160 170 180 DTNYHYIVPE FRKDQKFSPI SEKAIREYTE AKVVIGKAAK PVLLGPVSYL LAGKEKEAGF 190 200 210 220 230 240 NRIELIENLL PVYTKLLATL QQNGAEWIQL DEPFLVMDLT VQERKTYETT YTALRNLFPS 250 260 270 280 290 300 LKFIVATYFE CTGTNIDIAA KLPVDALHLD LVRCPSQLSD ILTPAFISSN TMLSLGVVDG 310 320 330 340 350 360 RNVWKNDFST SSTFITKAIA ALGKERVMIA PSCSLLHVPC DLDNEKNGNV LTPEIKNWMA 370 380 390 400 410 420 FAKQKLHEVA ALKKLNRAPM QSEELRLLKA NTESIENRAK SKLIHKQHVK ARVKAVQPSD 430 440 450 460 470 480 SQRKSLFKSR QQIQEKALQL PLMPTTTIGS FPQTDEVRAN RAKYKKGDIT LAQYEDFVKT 490 500 510 520 530 540 EMTKAIEWQE KIGIDVLVHG EFERNDMVEY FGEQLKGYVF SENGWVQSYG SRCVKPPIIY 550 560 570 580 590 600 GDIERTEPMT VAWTTLAQSL TKKYMKGMLT GPVTILQWSF VRDDQPRRDT CMQIAFAIRD 610 620 630 640 650 660 EVVDLEAAGI KIIQIDEPAI REGLPLRREN WETYLNWAVE CFRISASGVK DETQIHTHMC 670 680 690 700 710 720 YSEFNDIIKN IAAMDADVIT IETSRSQMEL LDAFVDFNYP NEIGPGVYDI HSPRIPTTDE 730 740 750 760 770 MADLLNKAMH VLPVRNIWVN PDCGLKTRRW PETEAALINM VAAAQKIRKE ITTVV

References

[1]

"Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii." Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P., Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R., Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B., McBride M.J. Appl. Environ. Microbiol. 73:3536-3546(2007) [PubMed: 17400776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000383 Genomic DNA. Translation: ABG60559.1.
RefSeq	YP_679902.1.
3D structure databases
SMR	Q11PV4. Positions 3-770.
ModBase	Search...
Protein-protein interaction databases
STRING	Q11PV4.
Genome annotation databases
GeneID	4186690.
GenomeReviews	Gene locus CHU_3320 in contig CP000383_GR.
KEGG	chu:CHU_3320.
NMPDR	fig|269798.12.peg.3166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	SFVRNDI.
Enzyme and pathway databases
BioCyc	CHUT269798:CHU_3320-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_CYTH3

Accession

Primary (citable) accession number: Q11PV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	August 22, 2006
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)