Q11PQ4 (SYE_CYTH3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	CHU_3373

Organism

Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) [Complete proteome] [HAMAP]

Taxonomic identifier

269798 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Cytophagia › Cytophagales › Cytophagaceae › Cytophaga

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000330966
Regions
	Motif	14 – 24	11	"HIGH" region HAMAP MF_00022_B
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11PQ4 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: DC98D665678A2C4D	FASTA	517	58,622
	10 20 30 40 50 60 MSDTNKQVRV RFAPSPTGPL HIGGVRTALY NYLFARKMGG KMLLRIEDTD QNRFVPGAEA 70 80 90 100 110 120 YIQEALAWVG IVIDEGAGVG GPHAPYKQSE RKPMYREYAE KLIAEGNAYY AFDTSEELEA 130 140 150 160 170 180 MKERLKAAKV ASPSYNMVTR MQMNNSLTLP EDEVKRRLDA GDEYVIRLKV PRKEEIRLND 190 200 210 220 230 240 MIRGWVVVHS STIDDKVLLK SDGMPTYHLA NIVDDHLMEI THVIRGEEWL PSAPLHVLLY 250 260 270 280 290 300 RFLGWEDTMP QFAHLPLLLK PDGNGKLSKR DADAGGFPIF PLDWKDPASG DTWIGFKQQG 310 320 330 340 350 360 YLQEATTNFL AFLGWNPGTQ QELFTMDELI EAFTVERIGK SGTKFDINKA KWYNQQYMRQ 370 380 390 400 410 420 LPDETLTRLL KEEADKHQAK YDAAKLPLIA QMLKERLTFA KDYWIEGQFF FEAPETFDEK 430 440 450 460 470 480 VASTKWNAEA VTVISAYKEA LAAYTGEFNA ENVKHVLHEV LEKADVKIGK IMQALRLALT 490 500 510 GAGAGPDLMQ FIEIVGKEEA IKRMQFALIT LSEKVNS

References

[1]

"Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii." Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P., Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R., Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B., McBride M.J. Appl. Environ. Microbiol. 73:3536-3546(2007) [PubMed: 17400776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33406 / NCIMB 9469.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000383 Genomic DNA. Translation: ABG60609.1.
RefSeq	YP_679952.1. NC_008255.1.
3D structure databases
ProteinModelPortal	Q11PQ4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q11PQ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4187162.
GenomeReviews	Gene locus CHU_3373 in contig CP000383_GR.
KEGG	chu:CHU_3373.
NMPDR	fig|269798.12.peg.3216.
PATRIC	21597625. VBICytHut34013_3357.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	VRFRMPD.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	CHUT269798:CHU_3373-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_CYTH3

Accession

Primary (citable) accession number: Q11PQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program