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Q11NV4

- SYI_CYTH3

UniProt

Q11NV4 - SYI_CYTH3

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Protein

Isoleucine--tRNA ligase

Gene
ileS, CHU_3676
Organism
Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei661 – 6611ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCHUT269798:GJ83-3669-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CHU_3676
OrganismiCytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Taxonomic identifieri269798 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeCytophaga
ProteomesiUP000001822: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11101110Isoleucine--tRNA ligaseUniRule annotationPRO_1000070895Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi269798.CHU_3676.

Structurei

3D structure databases

ProteinModelPortaliQ11NV4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionUniRule annotationAdd
BLAST
Motifi658 – 6625"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q11NV4-1 [UniParc]FASTAAdd to Basket

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MKYNEKKTEL SEVGKEVQQF WNDKKIFEKS VETREGNPTF TFYEGPPSAN     50
GTPGIHHVMG RTVKDIFCRF KTMQGFQVKR KGGWDTHGLP VELQVEKELG 100
ITKEDIGKKI TVEQYNQKCR EAVMKYKSQW DELTVKMGYW VDLEKPYITF 150
ENNYIESVWY LLKEFHQKKL LYKGYTIQPY SPAAGTGLSS HELNQPGTYK 200
DVRDTSAVAQ FKLKANPKFA DNTYFLAWTT TPWTLPSNSA LAVGENIEYV 250
LVSTFNPYTF KPVQVILAKA LLGKYFSEKA KDLSLEAYKD GDKLIPFKIL 300
QSYKGKDLVG IEYEQLMPYL QPETPAFRVI AGDFVSTEDG TGIVHIAPTF 350
GADDARVAKL AGIPSIVTKD DRGNEYPLVD KRGRFTKEVT DFAGEYVKEA 400
YLTDEEKEAE RVKQGRDKYL SVDERISIKL KEANRAFKVE KYEHSYPHCW 450
RTDKPVLYYP LDSWFIKTTA KKERLVELNK TINWKPESTG VGRFGNWLEN 500
LVDWNLSRSR YWGTPLPIWR SEDGSEEKCI GSIEELKTEI AKAQKAGIET 550
ATDIKDLHRP YVDNIVLVSD SGKPMKRELD LIDVWFDSGA MPYAQWHYPF 600
ENKELFNNNY PADFIAEGVD QTRGWFFTLH AISGMLYDKV AFKNVIANGL 650
VLDKNGNKMS KRVGNVVNPF ETIDKYGPDA TRWYMITNAP PWDNLKFNLD 700
GITEVQRRFF GTLQNTYSFF ALYANLDNFT FAEAEIPLAQ RTESDRWILS 750
KLQSLVKDVA DAYSDYEPTK AGRAIQDFVV DDLSNWYVRL NRKRFWKGEY 800
NADKTAAYQT LYTCLETVAK LGAPIAPFYM DKLFSDLNQV SKKNAVESVH 850
LADFPKVNEA FLDVELEERM SLAQRISSLV HSIRKAQTIK VRQPLSRILI 900
PILQPHLKAQ IQAVEDLIKN EVNIKAVEYI EDTSGVVIKT IKPNFKKLGK 950
EYGAKLKEIG NAIAELRAED ITAIERNVFE LKLADGTVIP ITSEDVEIRS 1000
QDIPGWSVAS EGGITVALDI TLSDDLRKEG IARDVVNRVQ NLRKDMGLEV 1050
QDKIRITIQK VDELINSALS ANQEYICTET QAFSLELVEK LADGKEVEMD 1100
EQTLIMKIEK 1110
Length:1,110
Mass (Da):126,860
Last modified:August 22, 2006 - v1
Checksum:iBF665BCB606DE8B9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000383 Genomic DNA. Translation: ABG60909.1.
RefSeqiYP_680252.1. NC_008255.1.

Genome annotation databases

EnsemblBacteriaiABG60909; ABG60909; CHU_3676.
GeneIDi4184649.
KEGGichu:CHU_3676.
PATRICi21598233. VBICytHut34013_3661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000383 Genomic DNA. Translation: ABG60909.1 .
RefSeqi YP_680252.1. NC_008255.1.

3D structure databases

ProteinModelPortali Q11NV4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269798.CHU_3676.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG60909 ; ABG60909 ; CHU_3676 .
GeneIDi 4184649.
KEGGi chu:CHU_3676.
PATRICi 21598233. VBICytHut34013_3661.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CHUT269798:GJ83-3669-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33406 / NCIMB 9469.

Entry informationi

Entry nameiSYI_CYTH3
AccessioniPrimary (citable) accession number: Q11NV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 22, 2006
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi