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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei661ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCHUT269798:G1G6N-3604-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CHU_3676
OrganismiCytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Taxonomic identifieri269798 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeCytophaga
Proteomesi
  • UP000001822 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000708951 – 1110Isoleucine--tRNA ligaseAdd BLAST1110

Proteomic databases

PRIDEiQ11NV4

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269798.CHU_3676

Structurei

3D structure databases

ProteinModelPortaliQ11NV4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 57"HIGH" regionAdd BLAST11
Motifi658 – 662"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 2 hits
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

Q11NV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYNEKKTEL SEVGKEVQQF WNDKKIFEKS VETREGNPTF TFYEGPPSAN
60 70 80 90 100
GTPGIHHVMG RTVKDIFCRF KTMQGFQVKR KGGWDTHGLP VELQVEKELG
110 120 130 140 150
ITKEDIGKKI TVEQYNQKCR EAVMKYKSQW DELTVKMGYW VDLEKPYITF
160 170 180 190 200
ENNYIESVWY LLKEFHQKKL LYKGYTIQPY SPAAGTGLSS HELNQPGTYK
210 220 230 240 250
DVRDTSAVAQ FKLKANPKFA DNTYFLAWTT TPWTLPSNSA LAVGENIEYV
260 270 280 290 300
LVSTFNPYTF KPVQVILAKA LLGKYFSEKA KDLSLEAYKD GDKLIPFKIL
310 320 330 340 350
QSYKGKDLVG IEYEQLMPYL QPETPAFRVI AGDFVSTEDG TGIVHIAPTF
360 370 380 390 400
GADDARVAKL AGIPSIVTKD DRGNEYPLVD KRGRFTKEVT DFAGEYVKEA
410 420 430 440 450
YLTDEEKEAE RVKQGRDKYL SVDERISIKL KEANRAFKVE KYEHSYPHCW
460 470 480 490 500
RTDKPVLYYP LDSWFIKTTA KKERLVELNK TINWKPESTG VGRFGNWLEN
510 520 530 540 550
LVDWNLSRSR YWGTPLPIWR SEDGSEEKCI GSIEELKTEI AKAQKAGIET
560 570 580 590 600
ATDIKDLHRP YVDNIVLVSD SGKPMKRELD LIDVWFDSGA MPYAQWHYPF
610 620 630 640 650
ENKELFNNNY PADFIAEGVD QTRGWFFTLH AISGMLYDKV AFKNVIANGL
660 670 680 690 700
VLDKNGNKMS KRVGNVVNPF ETIDKYGPDA TRWYMITNAP PWDNLKFNLD
710 720 730 740 750
GITEVQRRFF GTLQNTYSFF ALYANLDNFT FAEAEIPLAQ RTESDRWILS
760 770 780 790 800
KLQSLVKDVA DAYSDYEPTK AGRAIQDFVV DDLSNWYVRL NRKRFWKGEY
810 820 830 840 850
NADKTAAYQT LYTCLETVAK LGAPIAPFYM DKLFSDLNQV SKKNAVESVH
860 870 880 890 900
LADFPKVNEA FLDVELEERM SLAQRISSLV HSIRKAQTIK VRQPLSRILI
910 920 930 940 950
PILQPHLKAQ IQAVEDLIKN EVNIKAVEYI EDTSGVVIKT IKPNFKKLGK
960 970 980 990 1000
EYGAKLKEIG NAIAELRAED ITAIERNVFE LKLADGTVIP ITSEDVEIRS
1010 1020 1030 1040 1050
QDIPGWSVAS EGGITVALDI TLSDDLRKEG IARDVVNRVQ NLRKDMGLEV
1060 1070 1080 1090 1100
QDKIRITIQK VDELINSALS ANQEYICTET QAFSLELVEK LADGKEVEMD
1110
EQTLIMKIEK
Length:1,110
Mass (Da):126,860
Last modified:August 22, 2006 - v1
Checksum:iBF665BCB606DE8B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000383 Genomic DNA Translation: ABG60909.1
RefSeqiWP_011587014.1, NZ_FPJX01000004.1

Genome annotation databases

EnsemblBacteriaiABG60909; ABG60909; CHU_3676
KEGGichu:CHU_3676

Similar proteinsi

Entry informationi

Entry nameiSYI_CYTH3
AccessioniPrimary (citable) accession number: Q11NV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 22, 2006
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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