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Q11NV4

- SYI_CYTH3

UniProt

Q11NV4 - SYI_CYTH3

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei661 – 6611ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCHUT269798:GJ83-3669-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CHU_3676
OrganismiCytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Taxonomic identifieri269798 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeCytophaga
ProteomesiUP000001822: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11101110Isoleucine--tRNA ligasePRO_1000070895Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269798.CHU_3676.

Structurei

3D structure databases

ProteinModelPortaliQ11NV4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi658 – 6625"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q11NV4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKYNEKKTEL SEVGKEVQQF WNDKKIFEKS VETREGNPTF TFYEGPPSAN
60 70 80 90 100
GTPGIHHVMG RTVKDIFCRF KTMQGFQVKR KGGWDTHGLP VELQVEKELG
110 120 130 140 150
ITKEDIGKKI TVEQYNQKCR EAVMKYKSQW DELTVKMGYW VDLEKPYITF
160 170 180 190 200
ENNYIESVWY LLKEFHQKKL LYKGYTIQPY SPAAGTGLSS HELNQPGTYK
210 220 230 240 250
DVRDTSAVAQ FKLKANPKFA DNTYFLAWTT TPWTLPSNSA LAVGENIEYV
260 270 280 290 300
LVSTFNPYTF KPVQVILAKA LLGKYFSEKA KDLSLEAYKD GDKLIPFKIL
310 320 330 340 350
QSYKGKDLVG IEYEQLMPYL QPETPAFRVI AGDFVSTEDG TGIVHIAPTF
360 370 380 390 400
GADDARVAKL AGIPSIVTKD DRGNEYPLVD KRGRFTKEVT DFAGEYVKEA
410 420 430 440 450
YLTDEEKEAE RVKQGRDKYL SVDERISIKL KEANRAFKVE KYEHSYPHCW
460 470 480 490 500
RTDKPVLYYP LDSWFIKTTA KKERLVELNK TINWKPESTG VGRFGNWLEN
510 520 530 540 550
LVDWNLSRSR YWGTPLPIWR SEDGSEEKCI GSIEELKTEI AKAQKAGIET
560 570 580 590 600
ATDIKDLHRP YVDNIVLVSD SGKPMKRELD LIDVWFDSGA MPYAQWHYPF
610 620 630 640 650
ENKELFNNNY PADFIAEGVD QTRGWFFTLH AISGMLYDKV AFKNVIANGL
660 670 680 690 700
VLDKNGNKMS KRVGNVVNPF ETIDKYGPDA TRWYMITNAP PWDNLKFNLD
710 720 730 740 750
GITEVQRRFF GTLQNTYSFF ALYANLDNFT FAEAEIPLAQ RTESDRWILS
760 770 780 790 800
KLQSLVKDVA DAYSDYEPTK AGRAIQDFVV DDLSNWYVRL NRKRFWKGEY
810 820 830 840 850
NADKTAAYQT LYTCLETVAK LGAPIAPFYM DKLFSDLNQV SKKNAVESVH
860 870 880 890 900
LADFPKVNEA FLDVELEERM SLAQRISSLV HSIRKAQTIK VRQPLSRILI
910 920 930 940 950
PILQPHLKAQ IQAVEDLIKN EVNIKAVEYI EDTSGVVIKT IKPNFKKLGK
960 970 980 990 1000
EYGAKLKEIG NAIAELRAED ITAIERNVFE LKLADGTVIP ITSEDVEIRS
1010 1020 1030 1040 1050
QDIPGWSVAS EGGITVALDI TLSDDLRKEG IARDVVNRVQ NLRKDMGLEV
1060 1070 1080 1090 1100
QDKIRITIQK VDELINSALS ANQEYICTET QAFSLELVEK LADGKEVEMD
1110
EQTLIMKIEK
Length:1,110
Mass (Da):126,860
Last modified:August 22, 2006 - v1
Checksum:iBF665BCB606DE8B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000383 Genomic DNA. Translation: ABG60909.1.
RefSeqiYP_680252.1. NC_008255.1.

Genome annotation databases

EnsemblBacteriaiABG60909; ABG60909; CHU_3676.
GeneIDi4184649.
KEGGichu:CHU_3676.
PATRICi21598233. VBICytHut34013_3661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000383 Genomic DNA. Translation: ABG60909.1 .
RefSeqi YP_680252.1. NC_008255.1.

3D structure databases

ProteinModelPortali Q11NV4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269798.CHU_3676.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG60909 ; ABG60909 ; CHU_3676 .
GeneIDi 4184649.
KEGGi chu:CHU_3676.
PATRICi 21598233. VBICytHut34013_3661.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CHUT269798:GJ83-3669-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33406 / NCIMB 9469.

Entry informationi

Entry nameiSYI_CYTH3
AccessioniPrimary (citable) accession number: Q11NV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 22, 2006
Last modified: November 26, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3