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Q11NV4

- SYI_CYTH3

UniProt

Q11NV4 - SYI_CYTH3

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (22 Aug 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei661 – 6611ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCHUT269798:GJ83-3669-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CHU_3676
    OrganismiCytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
    Taxonomic identifieri269798 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeCytophaga
    ProteomesiUP000001822: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11101110Isoleucine--tRNA ligasePRO_1000070895Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi269798.CHU_3676.

    Structurei

    3D structure databases

    ProteinModelPortaliQ11NV4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi47 – 5711"HIGH" regionAdd
    BLAST
    Motifi658 – 6625"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiKWIISEI.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q11NV4-1 [UniParc]FASTAAdd to Basket

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    MKYNEKKTEL SEVGKEVQQF WNDKKIFEKS VETREGNPTF TFYEGPPSAN     50
    GTPGIHHVMG RTVKDIFCRF KTMQGFQVKR KGGWDTHGLP VELQVEKELG 100
    ITKEDIGKKI TVEQYNQKCR EAVMKYKSQW DELTVKMGYW VDLEKPYITF 150
    ENNYIESVWY LLKEFHQKKL LYKGYTIQPY SPAAGTGLSS HELNQPGTYK 200
    DVRDTSAVAQ FKLKANPKFA DNTYFLAWTT TPWTLPSNSA LAVGENIEYV 250
    LVSTFNPYTF KPVQVILAKA LLGKYFSEKA KDLSLEAYKD GDKLIPFKIL 300
    QSYKGKDLVG IEYEQLMPYL QPETPAFRVI AGDFVSTEDG TGIVHIAPTF 350
    GADDARVAKL AGIPSIVTKD DRGNEYPLVD KRGRFTKEVT DFAGEYVKEA 400
    YLTDEEKEAE RVKQGRDKYL SVDERISIKL KEANRAFKVE KYEHSYPHCW 450
    RTDKPVLYYP LDSWFIKTTA KKERLVELNK TINWKPESTG VGRFGNWLEN 500
    LVDWNLSRSR YWGTPLPIWR SEDGSEEKCI GSIEELKTEI AKAQKAGIET 550
    ATDIKDLHRP YVDNIVLVSD SGKPMKRELD LIDVWFDSGA MPYAQWHYPF 600
    ENKELFNNNY PADFIAEGVD QTRGWFFTLH AISGMLYDKV AFKNVIANGL 650
    VLDKNGNKMS KRVGNVVNPF ETIDKYGPDA TRWYMITNAP PWDNLKFNLD 700
    GITEVQRRFF GTLQNTYSFF ALYANLDNFT FAEAEIPLAQ RTESDRWILS 750
    KLQSLVKDVA DAYSDYEPTK AGRAIQDFVV DDLSNWYVRL NRKRFWKGEY 800
    NADKTAAYQT LYTCLETVAK LGAPIAPFYM DKLFSDLNQV SKKNAVESVH 850
    LADFPKVNEA FLDVELEERM SLAQRISSLV HSIRKAQTIK VRQPLSRILI 900
    PILQPHLKAQ IQAVEDLIKN EVNIKAVEYI EDTSGVVIKT IKPNFKKLGK 950
    EYGAKLKEIG NAIAELRAED ITAIERNVFE LKLADGTVIP ITSEDVEIRS 1000
    QDIPGWSVAS EGGITVALDI TLSDDLRKEG IARDVVNRVQ NLRKDMGLEV 1050
    QDKIRITIQK VDELINSALS ANQEYICTET QAFSLELVEK LADGKEVEMD 1100
    EQTLIMKIEK 1110
    Length:1,110
    Mass (Da):126,860
    Last modified:August 22, 2006 - v1
    Checksum:iBF665BCB606DE8B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000383 Genomic DNA. Translation: ABG60909.1.
    RefSeqiYP_680252.1. NC_008255.1.

    Genome annotation databases

    EnsemblBacteriaiABG60909; ABG60909; CHU_3676.
    GeneIDi4184649.
    KEGGichu:CHU_3676.
    PATRICi21598233. VBICytHut34013_3661.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000383 Genomic DNA. Translation: ABG60909.1 .
    RefSeqi YP_680252.1. NC_008255.1.

    3D structure databases

    ProteinModelPortali Q11NV4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269798.CHU_3676.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABG60909 ; ABG60909 ; CHU_3676 .
    GeneIDi 4184649.
    KEGGi chu:CHU_3676.
    PATRICi 21598233. VBICytHut34013_3661.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi KWIISEI.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CHUT269798:GJ83-3669-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33406 / NCIMB 9469.

    Entry informationi

    Entry nameiSYI_CYTH3
    AccessioniPrimary (citable) accession number: Q11NV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: August 22, 2006
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3