Q11I44 (SYE1_MESSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Meso_1535

Organism

Mesorhizobium sp. (strain BNC1) [Complete proteome] [HAMAP]

Taxonomic identifier

266779 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Chelativorans

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367705
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	250 – 254	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	253	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11I44 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 24EF53F9CE3F9299	FASTA	456	50,979
	10 20 30 40 50 60 MTVTVRFAPS PTGQIHIGNA RTALFNWLFA RRAGGSFVLR FDDTDVERSR REYADQIEID 70 80 90 100 110 120 LAWLGIKPDL TVRQSERFGL YNQAVEKLKE AGRLYPCYET AEELELRRKI RLSRRLPPIY 130 140 150 160 170 180 GREALKLTEE QKAAYEAEGR KPHWRFLLPN FDSDPFSTGR TEIHWDDVIR GPQTVDLASM 190 200 210 220 230 240 SDPVLVREDG TFLYTLPSVV DDIDLGITHV IRGDDHVTNT GAQIALFEAL GAKAPCFGHH 250 260 270 280 290 300 NLLTTVTGEG LSKRTGALSI ASLRKAGIEP MAVASLAVLI GTSEAVTAYR DMEELAAHFE 310 320 330 340 350 360 PASSSKSSAK FDPAELEALS RSLIQAMPFE AAAPRLAAMG VEGSIAEPFW NAVRGNIDKL 370 380 390 400 410 420 SEAAEWWHIV MKGPEPTEFT EDDRNFVNEA FDLLPPEPWD RETWRSWTEK VKEKTGRKGK 430 440 450 ALFRPLRLAL TGRESGPELA DLLPLLGRER TLARQP

References

[1]

"Complete sequence of chromosome of Mesorhizobium sp. BNC1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BNC1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000390 Genomic DNA. Translation: ABG62931.1.
RefSeq	YP_674096.1. NC_008254.1.
3D structure databases
ProteinModelPortal	Q11I44.
ModBase	Search...
Protein-protein interaction databases
STRING	Q11I44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4180580.
GenomeReviews	Gene locus Meso_1535 in contig CP000390_GR.
KEGG	mes:Meso_1535.
NMPDR	fig|266779.1.peg.282.
PATRIC	21343106. VBICheSp72577_2183.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DQERIEW.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	MSP266779:MESO_1535-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_MESSB

Accession

Primary (citable) accession number: Q11I44

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program