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Reviewed, UniProtKB/Swiss-Prot Q11HV9 (ISPDF_MESSB)

Last modified June 16, 2009. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12
Gene names
Name: ispDF
Ordered Locus Names: Meso_1621
OrganismMesorhizobium sp. (strain BNC1) [Complete proteome] [HAMAP]
Taxonomic identifier266779 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

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Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520

Cofactor

Divalent metal cations By similarity.

Pathway

Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520

Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Sequence similarities

In the N-terminal section; belongs to the ispD family.

In the C-terminal section; belongs to the ispF family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 408408Bifunctional enzyme ispD/ispF HAMAP MF_01520
PRO_0000292855

Regions

Region1 – 2472472-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
Region248 – 4081612-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520

Sites

Metal binding2541Divalent metal cation By similarity
Metal binding2561Divalent metal cation By similarity
Metal binding2881Divalent metal cation By similarity
Site261Transition state stabilizer By similarity
Site351Transition state stabilizer By similarity
Site1661Positions MEP for the nucleophilic attack By similarity
Site2231Positions MEP for the nucleophilic attack By similarity
Site2801Transition state stabilizer By similarity
Site3791Transition state stabilizer By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q11HV9-1 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 92FC006A88AAC43A

FASTA40843,219
        10         20         30         40         50         60 
MNAPFEKDRR NRRVAVVVVA AGRGERAGQA QNGPKQYRLI GGKPVIRHTL EVLAKSKRVG 

        70         80         90        100        110        120 
TIAVAVHPED SSLFNQAREG LDGDIRAVHG GASRQESTRL ALAALEEVKP DIVLIHDGVR 

       130        140        150        160        170        180 
PFIDEALIER VIEAVGEDSG ALPALPVSDT LKRSGVNGII TGTVPRDGLF AAQTPQGFPF 

       190        200        210        220        230        240 
RPIYEAHQKA WQSGRSDFTD DAAIAEWAGI SVRLVSGSPD NVKLTWAKDI ALADQRLGPA 

       250        260        270        280        290        300 
MTELPDIRVG NGYDVHGFGP GGHVTLCGVQ IPHGKTLMGH SDADVGLHAL TDALLATCGA 

       310        320        330        340        350        360 
GDIGTHFPPS DPQWKGAKSR IFVEHAVNLL RARGGRIANA DVTLICEAPR IGPYREAMLA 

       370        380        390        400 
ELSDMLQISP ERVSVKATTN EGLGFIGRAE GIAAIATATV AYPGSLGN

« Hide

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References

[1]"Complete sequence of chromosome of Mesorhizobium sp. BNC1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000390 Genomic DNA. Translation: ABG63016.1.
RefSeqYP_674181.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID4179827.
GenomeReviewsGene locus Meso_1621 in contig CP000390_GR.
KEGGmes:Meso_1621.
NMPDRfig|266779.1.peg.367.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ11HV9.
OMAQ11HV9. IVLIHDA.

Enzyme and pathway databases

BioCycMSP266779:MESO_1621-MON.

Family and domain databases

HAMAPMF_01520.
[Tree]
InterProIPR001228. ISPD_synthase.
IPR018294. ISPD_synthase_CS.
IPR003526. MECDP_synthase_core.
[Graphical view]
Gene3DG3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
PfamPF01128. IspD. 1 hit.
PF02542. YgbB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00453. ispD. 1 hit.
TIGR00151. ispF. 1 hit.
PROSITEPS01295. ISPD. 1 hit.
PS01350. ISPF. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameISPDF_MESSB
AccessionPrimary (citable) accession number: Q11HV9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: August 22, 2006
Last modified: June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents