Reviewed, UniProtKB/Swiss-Prot Q11GS0 (MURE_MESSB)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	Meso_2012

Organism

Mesorhizobium sp. (strain BNC1) [Complete proteome] [HAMAP]

Taxonomic identifier

266779 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000012372
Regions
	Nucleotide binding	107 – 113	7	ATP Potential
	Region	149 – 150	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	404 – 407	4	Meso-diaminopimelate binding By similarity
	Motif	404 – 407	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	29	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	176	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	182	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	184	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	380	1	Meso-diaminopimelate By similarity
	Binding site	452	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	456	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	216	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11GS0-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: A34DDB6E426467D5	FASTA	483	51,101
	10 20 30 40 50 60 MKLGEFADIL PLSGSIAGET TISGLASDSR RVNAGDLFFA LQGSKADGAA YAADAAGRGA 70 80 90 100 110 120 VAIIARPGTV GDVGVPVIEA DDPRHVLALA SARLYGAQPA TMVAVTGTSG KTSVAAFTRQ 130 140 150 160 170 180 IWEKAGLAAA SIGTTGVVAP GRDEYGELTT PDPVSLHKLL RELADAGVTH ASMEASSHGL 190 200 210 220 230 240 DQRRLDGVKL AAGGFTNLGR DHMDYHPTVE HYHQSKMRLF KTLLPKGAPA VIFADDPWSE 250 260 270 280 290 300 PTEKVALAAG LQLLTVGRRG TFLTIKRVEH ERHRQRTEIE HEGMIYDIML PLAGDFQVGN 310 320 330 340 350 360 ALVAAGLAIS TGLPADKAVA ALEHLKGASG RLELTGTTED GAQIYVDYAH KPDALENVLQ 370 380 390 400 410 420 AVRPFTTGRV IVVFGCGGDR DPGKRPIMGE IARRLADVVI VTDDNPRSEV PAEIRRAILE 430 440 450 460 470 480 AVPEATEIGD RREAIRTAVA MLNSGDTLIV AGKGHEIGQE IAGTKLPFSD HEEVRRALKE RTA

References

[1]

"Complete sequence of chromosome of Mesorhizobium sp. BNC1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000390 Genomic DNA. Translation: ABG63405.1.
RefSeq	YP_674570.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4182805.
GenomeReviews	Gene locus Meso_2012 in contig CP000390_GR.
KEGG	mes:Meso_2012.
NMPDR	fig|266779.1.peg.607.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q11GS0.
OMA	Q11GS0. ERCGIVG.
Enzyme and pathway databases
BioCyc	MSP266779:MESO_2012-MON.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_MESSB

Accession

Primary (citable) accession number: Q11GS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)