Q11EB8 (Q11EB8_MESSB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Meso_2881

Organism

Mesorhizobium sp. (strain BNC1) [Complete proteome] [HAMAP]

Taxonomic identifier

266779 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Chelativorans

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 RuleBase RU004252 SAAS SAAS003694
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 NAD HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 EMBL ABG64257.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	42 – 49	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	44	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	Q11EB8 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: CB72F3872F3310DC	FASTA	330	36,194
	10 20 30 40 50 60 MNSTAPFSVA TLAIDPAAEV ERIVAALREQ LRSLRKRGLV LGVSGGIDSS VSTALAVRAV 70 80 90 100 110 120 GAKNVCCLFM PENDSDPESL ELGRLLTNTF GIEGIVEDIG PALRAIGCYE RRDGFIRKLV 130 140 150 160 170 180 PDYGPGWASK IVISNALEGA AYNISWLVVQ DPEGNQSRHR MTPEVYLGIV AATNMKQRTR 190 200 210 220 230 240 KQIEYYHADR LNFAVLGTPN RLEYDQGFFV KNGDGAADVK PIAHLYKSQV YQLAEYLGVP 250 260 270 280 290 300 EVICRRPPTT DTYSLAQTQE EFYFSLPYDR MDLCLYGLNH GLPASAVAAA ASLTEEQLQS 310 320 330 VWADVAAKRK ATRYLHLPPL LVDKVSEVGA

References

[1]

"Complete sequence of chromosome of Mesorhizobium sp. BNC1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BNC1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000390 Genomic DNA. Translation: ABG64257.1.
RefSeq	YP_675422.1. NC_008254.1.
3D structure databases
ProteinModelPortal	Q11EB8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q11EB8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4182879.
GenomeReviews	Gene locus Meso_2881 in contig CP000390_GR.
KEGG	mes:Meso_2881.
NMPDR	fig|266779.1.peg.1413.
PATRIC	21346018. VBICheSp72577_3622.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	AHLYKSQ.
ProtClustDB	PRK00876.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q11EB8_MESSB

Accession

Primary (citable) accession number: Q11EB8

Entry history

Integrated into UniProtKB/TrEMBL:	August 22, 2006
Last sequence update:	August 22, 2006
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)