Reviewed, UniProtKB/Swiss-Prot Q11E18 (HUTH_MESSB)

Names and origin

Protein names

Recommended name:
    Histidine ammonia-lyase
      Short name=Histidase
    EC=4.3.1.3

Gene names

Name:	hutH
Ordered Locus Names:	Meso_2985

Organism

Mesorhizobium sp. (strain BNC1) [Complete proteome] [HAMAP]

Taxonomic identifier

266779 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-histidine = urocanate + NH3. HAMAP MF_00229
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3. HAMAP MF_00229
Subcellular location	Cytoplasm Potential.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro histidine catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ammonia ligase activity Inferred from electronic annotation. Source: InterPro histidine ammonia-lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Histidine ammonia-lyase HAMAP MF_00229		PRO_1000021560
Amino acid modifications
	Modified residue	143	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	142 ↔ 144		5-imidazolinone (Ala-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11E18-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: B80D982815C6102C	FASTA	511	53,243
	10 20 30 40 50 60 MTIILHPGSV LLRDLATIYW TGVPARLDPS FDAGIVKAAD RIAEIAAGNA PVYGINTGFG 70 80 90 100 110 120 KLASIKIDSA DVAALQRNLV LSHCCGVGEA LPENVVRLMM ALKLVSLGRG ASGVRLELVR 130 140 150 160 170 180 LIEAMLARGV IPVIPEKGSV GASGDLAPLA HMAAVMMGHG EAFFGGERLN GATALLKAGL 190 200 210 220 230 240 QPVELAAKEG LALINGTQTS TALALAGLFR AHRAAQSALI TGAMSTDAAM GSSAPFHPEI 250 260 270 280 290 300 HTLRGHRGQI DTAEALRALL ENSPIRQSHI EGDERVQDPY CIRCQPQVDG ACLDLLRSVA 310 320 330 340 350 360 RTLEIEANAV TDNPLVLSDN SVVAGGNFHA EPVAFAADQI VLAICEIGAI AQRRIALLVD 370 380 390 400 410 420 PALSYGLPAF LAKKPGLNSG LMIAEVTSAA LMSENKQMSH PASVDSTPTS ANQEDHVSMA 430 440 450 460 470 480 CHGARRLLPM TENLFAIIGI EALCAAQGVE LRAPLATSPE LTKAIAAIRN VVPSLEEDRY 490 500 510 MANDLKAATV LIASGSLNES VSSGILPALE V

References

[1]

"Complete sequence of chromosome of Mesorhizobium sp. BNC1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000390 Genomic DNA. Translation: ABG64357.1.
RefSeq	YP_675522.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4181421.
GenomeReviews	Gene locus Meso_2985 in contig CP000390_GR.
KEGG	mes:Meso_2985.
NMPDR	fig|266779.1.peg.1513.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q11E18.
OMA	Q11E18. AHMAAVM.
Enzyme and pathway databases
BioCyc	MSP266779:MESO_2985-MON.
Family and domain databases
HAMAP	MF_00229. [Tree]
InterPro	IPR005921. HutH. IPR001106. Phe/His_NH3-lyase. [Graphical view]
Pfam	PF00221. PAL. 1 hit. [Graphical view]
TIGRFAMs	TIGR01225. hutH. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HUTH_MESSB

Accession

Primary (citable) accession number: Q11E18

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)