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Protein

Malate synthase G

Gene

glcB

Organism
Chelativorans sp. (strain BNC1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei274 – 2741Acetyl-CoAUniRule annotation
Binding sitei311 – 3111Acetyl-CoAUniRule annotation
Active sitei338 – 3381Proton acceptorUniRule annotation
Binding sitei338 – 3381GlyoxylateUniRule annotation
Metal bindingi430 – 4301MagnesiumUniRule annotation
Binding sitei430 – 4301GlyoxylateUniRule annotation
Metal bindingi458 – 4581MagnesiumUniRule annotation
Binding sitei539 – 5391Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei629 – 6291Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCSP266779:GI09-3980-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Meso_3915
OrganismiChelativorans sp. (strain BNC1)
Taxonomic identifieri266779 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeChelativorans
ProteomesiUP000001820: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 732732Malate synthase GPRO_1000130891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei615 – 6151Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi266779.Meso_3915.

Structurei

3D structure databases

ProteinModelPortaliQ11BE3.
SMRiQ11BE3. Positions 2-720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
Regioni455 – 4584Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q11BE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRVEINGL KIARELHDFA MNEVLPGTGV DAETFWHSLS QIVHTLSPRN
60 70 80 90 100
RALLSKRDDL QAKIDAWHRA NRAPSDLQAY EAFLREIGYL LPEGPDFKVT
110 120 130 140 150
TSDVDPEISQ IAGPQLVVPV MNARYALNAA NARWGSLYDA LYGTDAIPET
160 170 180 190 200
AGAERGKGFN PQRGEKVIAW ARKFLDESIP LRNASWADAA RLSVKDGKLA
210 220 230 240 250
VDFDGAAADL ADPAQFTGYS GEADSPREVV LARNGLHVRI IIDRNHPIGS
260 270 280 290 300
TDRAGIADVV MEAALTTIMD CEDSVAAVDA EDKVLAYRNW LGLMKGDLEE
310 320 330 340 350
TFQKGGTTVT RKLNQDIRLT APDGSPIKLP GRSLMLVRNV GHLMTNPAIL
360 370 380 390 400
DRDGKEVPEG IMDAMFTALI ALHDIGPNGR RMNSRAGSMY VVKPKMHGPE
410 420 430 440 450
EVAFAVELFG SVEDALGMRP NTIKMGIMDE ERRTTVNLKE CIRAASERVV
460 470 480 490 500
FINTGFLDRT GDEIHTSMEA GPMIRKGDMK QSTWIAAYEN WNVDIGLLCG
510 520 530 540 550
LSGRAQIGKG MWAMPDLMAA MLDQKIAHPK AGANTAWVPS PTAATLHATH
560 570 580 590 600
YHQVDVKAVQ EGLKSRTRAK LGDILSIPVA VRPNWSPEDI RQELDNNAQG
610 620 630 640 650
ILGYVVRWID QGVGCSKVPD INNVGLMEDR ATLRISSQHI ANWLHHGVVT
660 670 680 690 700
PEQVMETMKR MAEVVDSQNA GDPDYEPIAP NFEDSIAFQA ACDLVFKGRE
710 720 730
QPNGYTEPVL HARRLQKKAQ DRKGAAADSS RG
Length:732
Mass (Da):80,279
Last modified:August 22, 2006 - v1
Checksum:i7B62E9807F370B74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000390 Genomic DNA. Translation: ABG65282.1.
RefSeqiWP_011583223.1. NC_008254.1.
YP_676447.1. NC_008254.1.

Genome annotation databases

EnsemblBacteriaiABG65282; ABG65282; Meso_3915.
GeneIDi4181887.
KEGGimes:Meso_3915.
PATRICi21348220. VBICheSp72577_4708.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000390 Genomic DNA. Translation: ABG65282.1.
RefSeqiWP_011583223.1. NC_008254.1.
YP_676447.1. NC_008254.1.

3D structure databases

ProteinModelPortaliQ11BE3.
SMRiQ11BE3. Positions 2-720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266779.Meso_3915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG65282; ABG65282; Meso_3915.
GeneIDi4181887.
KEGGimes:Meso_3915.
PATRICi21348220. VBICheSp72577_4708.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciCSP266779:GI09-3980-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BNC1.

Entry informationi

Entry nameiMASZ_CHESB
AccessioniPrimary (citable) accession number: Q11BE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: August 22, 2006
Last modified: January 7, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.