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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Trichodesmium erythraeum (strain IMS101)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei251ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:Tery_0188
OrganismiTrichodesmium erythraeum (strain IMS101)
Taxonomic identifieri203124 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesMicrocoleaceaeTrichodesmium
Proteomesi
  • UP000008878 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei809 – 829HelicalSequence analysisAdd BLAST21
Transmembranei832 – 852HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003677971 – 881Glutamate--tRNA ligaseAdd BLAST881

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi203124.Tery_0188.

Structurei

3D structure databases

ProteinModelPortaliQ119Z5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 480Glutamyl-tRNA synthetaseAdd BLAST480
Regioni481 – 881UnknownAdd BLAST401

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionAdd BLAST11
Motifi248 – 252"KMSKS" region5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi510 – 772Thr-richAdd BLAST263

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
KOiK01885.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR025564. CAAD_dom.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF14159. CAAD. 1 hit.
PF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q119Z5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVRVRLAPS PTGNLHIGTA RTAVFNWLFA RNQKGKFILR IEDTDQERSR
60 70 80 90 100
PEYTDNILEG LSWLGLEWDE GPFYQCQRYE LYQKATQSLL DKGLAYRCYC
110 120 130 140 150
TAAELEEMRE AQKARKEAPR YDNRHRNLTD LEKKAFEAEG RQAVIRFRID
160 170 180 190 200
DDQIITWNDM VRGKVTWKGS DLGGDMVISR AAGGDEVGQP LYNMAVVVDD
210 220 230 240 250
MDMEITHVIR GEDHIANTAK QILLYEALGG KIPEFGHTPL ILNKEGRKLS
260 270 280 290 300
KRDGVTSISD FQELGFTPEA LANYMCLLGW TPPDATEEIF TLAQAGQNFS
310 320 330 340 350
FERVNKAGAK FDWDKLDWIS SQYLHNLPVK QLTDKLIPIW QKAGYELDPE
360 370 380 390 400
GDRSWLEQLV TLIRPSLTRL TDAVEMSELF FFPTVELNED AKTQMQNEDS
410 420 430 440 450
VKSINSILEI INADAPLLVA DVKQTIKKVT QKVHVKKGVV MRSLRASLTG
460 470 480 490 500
AMQGPDLIES WLLLHQKGFD ILRFKKSIGQ EIEDTKIEDT KKAETTPHKS
510 520 530 540 550
KGEGNVLLKT TTPKSPALSK EQTQTTKPPK KGQTATPVAT TPTATDVTEN
560 570 580 590 600
TSVGTQETQS QITTPVATTP TATDVTENTS VGTQETQSQI TTPVATTPTA
610 620 630 640 650
TDVTENTSVE TQETQSQITT PVATTPTATD VTENTSVETQ ETQSQITTPV
660 670 680 690 700
ATTPTATDVT ENTSVGTQET QSQITTPVAT TPTATDVTEN TSVETQETQS
710 720 730 740 750
QITTPVATTS TATDVTENTS VETQETQSQI TTPVATTPTA TDAETREQKV
760 770 780 790 800
ATQVETSILD DQKPVDTVTN QTVEVEQPNK IKEQFINIFF NFPDYINQLY
810 820 830 840 850
QQYQGQLKLF GWLALVILTF TFMAVVIEAL DGIPILSIIF ELIGVIYLVW
860 870 880
FVYRYLLKRS NRQELLDKIE NIKREIFGKP S
Length:881
Mass (Da):98,269
Last modified:August 22, 2006 - v1
Checksum:i1C675432E0CA07E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000393 Genomic DNA. Translation: ABG49679.1.

Genome annotation databases

EnsemblBacteriaiABG49679; ABG49679; Tery_0188.
KEGGiter:Tery_0188.
PATRICi23983588. VBITriEry99848_0240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000393 Genomic DNA. Translation: ABG49679.1.

3D structure databases

ProteinModelPortaliQ119Z5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203124.Tery_0188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG49679; ABG49679; Tery_0188.
KEGGiter:Tery_0188.
PATRICi23983588. VBITriEry99848_0240.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
KOiK01885.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR025564. CAAD_dom.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF14159. CAAD. 1 hit.
PF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_TRIEI
AccessioniPrimary (citable) accession number: Q119Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 22, 2006
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.