Q118M1 (ISPD_TRIEI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	Tery_0609

Organism

Trichodesmium erythraeum (strain IMS101) [Complete proteome] [HAMAP]

Taxonomic identifier

203124 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Oscillatoriales › Trichodesmium

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000022957
Sites
	Site	13	1	Transition state stabilizer By similarity
	Site	20	1	Transition state stabilizer By similarity
	Site	152	1	Positions MEP for the nucleophilic attack By similarity
	Site	208	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q118M1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: BCA35804BC832C6C	FASTA	226	24,660
	10 20 30 40 50 60 MNLLIPAAGI GRRMGGSRNK LLLTLLGKSL LSWTLEAAVK SDHITWIGII GQPIDFPDFQ 70 80 90 100 110 120 KNISDLSTDK HIELIEGGAT RQESVYNGLQ ALPSAAERVL IHDGARCLAT PELLDRCAVE 130 140 150 160 170 180 ILKCPGIIAS VPVKDTIKVV DPSGIIQDTP NRRNLWAAQT PQGFDVKLLK ECHKKGQILG 190 200 210 220 WEVTDDAALF EKCGLPVKIL EGEETNLKVT TPLDLKIAEF ILLKAL

References

[1]

"Complete sequence of Trichodesmium erythraeum IMS101." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IMS101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000393 Genomic DNA. Translation: ABG50053.1.
RefSeq	YP_720526.1. NC_008312.1.
3D structure databases
ProteinModelPortal	Q118M1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q118M1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4241980.
GenomeReviews	Gene locus Tery_0609 in contig CP000393_GR.
KEGG	ter:Tery_0609.
NMPDR	fig|203124.1.peg.3553.
PATRIC	23984643. VBITriEry99848_0762.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	SKVIVVC.
PhylomeDB	Q118M1.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	TERY203124:TERY_0609-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_TRIEI

Accession

Primary (citable) accession number: Q118M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program