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Q112A6 (Q112A6_TRIEI) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168 RuleBase RU003777
EC=2.4.2.29 HAMAP-Rule MF_00168 RuleBase RU003777
Alternative name(s):
Guanine insertion enzyme HAMAP-Rule MF_00168
tRNA-guanine transglycosylase HAMAP-Rule MF_00168
Gene names
Name:tgt HAMAP-Rule MF_00168
Ordered Locus Names:Tery_2456 EMBL ABG51668.1
OrganismTrichodesmium erythraeum (strain IMS101) [Complete proteome] [HAMAP] EMBL ABG51668.1
Taxonomic identifier203124 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Oscillatoriophycideae › Oscillatoriales › Trichodesmium ›

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168 SAAS SAAS004803 RuleBase RU003777

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00168 SAAS SAAS004803 RuleBase RU003777

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168 SAAS SAAS004803

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. HAMAP-Rule MF_00168 RuleBase RU003777

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site951Nucleophile By similarity HAMAP-Rule MF_00168
Metal binding3061Zinc By similarity HAMAP-Rule MF_00168
Metal binding3081Zinc By similarity HAMAP-Rule MF_00168
Metal binding3111Zinc By similarity HAMAP-Rule MF_00168
Metal binding3371Zinc By similarity HAMAP-Rule MF_00168
Binding site961Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
Q112A6 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: DEF925E8816DBF78

FASTA36741,037
        10         20         30         40         50         60 
MSQNFSFNSQ ARCSYTKARA GIFITPHGSI NTPRFMPVGT LANVKTLTPA QLEAAGAQMI 

        70         80         90        100        110        120 
LANTYHLHLQ PGESIVAGAG GLHKFMAWNR PILTDSGGFQ VFSLGDIRTI SEAGVKFRSP 

       130        140        150        160        170        180 
HDGSMIDLTP ERSIQIQNEL GGDVIMAFDE CPPYPASWEE VELATNRTYR WLQRCIEAHQ 

       190        200        210        220        230        240 
RQDQALFGIV QGGVYPELRS IAARQLVDLD LPGYAIGGVS VGEPGELIDH IVKVTTPFLP 

       250        260        270        280        290        300 
EDKPRYLMGV GTYREMVRAI AEGIDLFDCV IPTRLGRHGA ALVRGERWNL KNARFKEDFR 

       310        320        330        340        350        360 
PLDEFCPCYC CQNFSRAYLS HLVRAKEALG YTLLSLHNVT ELISFTQRIR DAILKDRFIT 


EFGSWLR

« Hide

References

[1]"Complete sequence of Trichodesmium erythraeum IMS101."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P. expand/collapse author list , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IMS101 EMBL ABG51668.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000393 Genomic DNA. Translation: ABG51668.1.
RefSeqYP_722141.1. NC_008312.1.

3D structure databases

ProteinModelPortalQ112A6.
SMRQ112A6. Positions 6-360.
ModBaseSearch...

Protein-protein interaction databases

STRING203124.Tery_2456.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG51668; ABG51668; Tery_2456.
GeneID4244640.
KEGGter:Tery_2456.
PATRIC23989355. VBITriEry99848_3106.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMAMSPERSI.

Enzyme and pathway databases

BioCycTERY203124:GJDR-2478-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ112A6_TRIEI
AccessionPrimary (citable) accession number: Q112A6
Entry history
Integrated into UniProtKB/TrEMBL: August 22, 2006
Last sequence update: August 22, 2006
Last modified: May 29, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info