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Protein

CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2

Gene

St3gal2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

It may be responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found in terminal carbohydrate groups of certain glycoproteins, oligosaccharides and glycolipids. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116SubstrateBy similarity1
Binding sitei157SubstrateBy similarity1
Binding sitei180SubstrateBy similarity1
Binding sitei240SubstrateBy similarity1
Binding sitei276SubstrateBy similarity1
Binding sitei280Substrate; via amide nitrogenBy similarity1
Binding sitei300Substrate; via amide nitrogenBy similarity1
Binding sitei309SubstrateBy similarity1
Binding sitei326SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.2. 5301.
2.4.99.4. 5301.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Chemistry databases

SwissLipidsiSLP:000001414.

Names & Taxonomyi

Protein namesi
Recommended name:
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2 (EC:2.4.99.4)
Short name:
Alpha 2,3-ST 2
Short name:
Beta-galactoside alpha-2,3-sialyltransferase 2
Alternative name(s):
Gal-NAc6S
Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
ST3Gal II
Short name:
ST3GalII
ST3GalA.2
Sialyltransferase 4B
Short name:
SIAT4-B
Gene namesi
Name:St3gal2
Synonyms:Siat4b, Siat5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68413. St3gal2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 350LumenalSequence analysisAdd BLAST323

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3638362.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492611 – 350CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2Add BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 75By similarity
Disulfide bondi72 ↔ 149By similarity
Glycosylationi92N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi152 ↔ 291By similarity
Glycosylationi211N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ11205.
PRIDEiQ11205.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024248.

Chemistry databases

BindingDBiQ11205.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
HOGENOMiHOG000126811.
HOVERGENiHBG054227.
InParanoidiQ11205.
KOiK03368.
PhylomeDBiQ11205.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q11205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCSLRVWFL SMAFLLVFIM SLLFTYSHHS MATLPYLDSG TLGGTHRVKL
60 70 80 90 100
VPGYTGQQRL VKEGLSGKSC TCSRCMGDAG TSEWFDSHFD SNISPVWTRD
110 120 130 140 150
NMNLTPDVQR WWMMLQPQFK SHNTNEVLEK LFQIVPGENP YRFRDPQQCR
160 170 180 190 200
RCAVVGNSGN LRGSGYGQEV DSHNFIMRMN QAPTVGFEKD VGSRTTHHFM
210 220 230 240 250
YPESAKNLPA NVSFVLVPFK ALDLMWIASA LSTGQIRFTY APVKSFLRVD
260 270 280 290 300
KEKVQIYNPA FFKYIHDRWT EHHGRYPSTG MLVLFFALHV CDEVNVYGFG
310 320 330 340 350
ADSRGNWHHY WENNRYAGEF RKTGVHDADF EAHIIDILAK ASKIEVYRGN
Length:350
Mass (Da):40,166
Last modified:October 1, 1996 - v1
Checksum:i87E6494FB02D0BE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76988 mRNA. Translation: CAA54293.1.
PIRiB54420.
RefSeqiNP_113883.2. NM_031695.2.
UniGeneiRn.33216.

Genome annotation databases

GeneIDi64442.
KEGGirno:64442.
UCSCiRGD:68413. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76988 mRNA. Translation: CAA54293.1.
PIRiB54420.
RefSeqiNP_113883.2. NM_031695.2.
UniGeneiRn.33216.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024248.

Chemistry databases

BindingDBiQ11205.
ChEMBLiCHEMBL3638362.
SwissLipidsiSLP:000001414.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Proteomic databases

PaxDbiQ11205.
PRIDEiQ11205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64442.
KEGGirno:64442.
UCSCiRGD:68413. rat.

Organism-specific databases

CTDi6483.
RGDi68413. St3gal2.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
HOGENOMiHOG000126811.
HOVERGENiHBG054227.
InParanoidiQ11205.
KOiK03368.
PhylomeDBiQ11205.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.2. 5301.
2.4.99.4. 5301.

Miscellaneous databases

PROiQ11205.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIA4B_RAT
AccessioniPrimary (citable) accession number: Q11205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.