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Protein

CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2

Gene

St3gal2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

It may be responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found in terminal carbohydrate groups of certain glycoproteins, oligosaccharides and glycolipids. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161SubstrateBy similarity
Binding sitei157 – 1571SubstrateBy similarity
Binding sitei180 – 1801SubstrateBy similarity
Binding sitei240 – 2401SubstrateBy similarity
Binding sitei276 – 2761SubstrateBy similarity
Binding sitei280 – 2801Substrate; via amide nitrogenBy similarity
Binding sitei300 – 3001Substrate; via amide nitrogenBy similarity
Binding sitei309 – 3091SubstrateBy similarity
Binding sitei326 – 3261SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.2. 5301.
2.4.99.4. 5301.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Chemistry

SwissLipidsiSLP:000001414.

Names & Taxonomyi

Protein namesi
Recommended name:
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2 (EC:2.4.99.4)
Short name:
Alpha 2,3-ST 2
Short name:
Beta-galactoside alpha-2,3-sialyltransferase 2
Alternative name(s):
Gal-NAc6S
Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
ST3Gal II
Short name:
ST3GalII
ST3GalA.2
Sialyltransferase 4B
Short name:
SIAT4-B
Gene namesi
Name:St3gal2
Synonyms:Siat4b, Siat5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68413. St3gal2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 350323LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2PRO_0000149261Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 75By similarity
Disulfide bondi72 ↔ 149By similarity
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence analysis
Disulfide bondi152 ↔ 291By similarity
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ11205.
PRIDEiQ11205.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024248.

Chemistry

BindingDBiQ11205.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
HOGENOMiHOG000126811.
HOVERGENiHBG054227.
InParanoidiQ11205.
KOiK03368.
PhylomeDBiQ11205.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q11205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCSLRVWFL SMAFLLVFIM SLLFTYSHHS MATLPYLDSG TLGGTHRVKL
60 70 80 90 100
VPGYTGQQRL VKEGLSGKSC TCSRCMGDAG TSEWFDSHFD SNISPVWTRD
110 120 130 140 150
NMNLTPDVQR WWMMLQPQFK SHNTNEVLEK LFQIVPGENP YRFRDPQQCR
160 170 180 190 200
RCAVVGNSGN LRGSGYGQEV DSHNFIMRMN QAPTVGFEKD VGSRTTHHFM
210 220 230 240 250
YPESAKNLPA NVSFVLVPFK ALDLMWIASA LSTGQIRFTY APVKSFLRVD
260 270 280 290 300
KEKVQIYNPA FFKYIHDRWT EHHGRYPSTG MLVLFFALHV CDEVNVYGFG
310 320 330 340 350
ADSRGNWHHY WENNRYAGEF RKTGVHDADF EAHIIDILAK ASKIEVYRGN
Length:350
Mass (Da):40,166
Last modified:October 1, 1996 - v1
Checksum:i87E6494FB02D0BE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76988 mRNA. Translation: CAA54293.1.
PIRiB54420.
RefSeqiNP_113883.2. NM_031695.2.
UniGeneiRn.33216.

Genome annotation databases

GeneIDi64442.
KEGGirno:64442.
UCSCiRGD:68413. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76988 mRNA. Translation: CAA54293.1.
PIRiB54420.
RefSeqiNP_113883.2. NM_031695.2.
UniGeneiRn.33216.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024248.

Chemistry

BindingDBiQ11205.
SwissLipidsiSLP:000001414.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Proteomic databases

PaxDbiQ11205.
PRIDEiQ11205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64442.
KEGGirno:64442.
UCSCiRGD:68413. rat.

Organism-specific databases

CTDi6483.
RGDi68413. St3gal2.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
HOGENOMiHOG000126811.
HOVERGENiHBG054227.
InParanoidiQ11205.
KOiK03368.
PhylomeDBiQ11205.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.2. 5301.
2.4.99.4. 5301.

Miscellaneous databases

PROiQ11205.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of cDNA for a new type of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase."
    Lee Y.-C., Kojima N., Wada E., Kurosawa N., Nakaoka T., Hamamoto T., Tsuji S.
    J. Biol. Chem. 269:10028-10033(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiSIA4B_RAT
AccessioniPrimary (citable) accession number: Q11205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.