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Protein

CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2

Gene

St3gal2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found in terminal carbohydrate groups of certain glycoproteins, oligosaccharides and glycolipids. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.2 Publications

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R.2 Publications

Kineticsi

Vmax is 10 fold higher with Gal-beta-1,3-GalNAc than with Gal-beta-1,3-GlcNAc as substrate.1 Publication
  1. KM=2.3 mM for Gal-beta-1,3-GlcNAc1 Publication
  2. KM=0.16 mM for Gal-beta-1,3-GalNAc1 Publication
  3. KM=0.63 mM for Gal-beta-1,3-GalNAc1 Publication
  4. KM=0.83 mM for GM11 Publication
  5. KM=0.67 mM for asialo-GM11 Publication

    pH dependencei

    Optimum pH is 6.4.1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei116SubstrateBy similarity1
    Binding sitei157SubstrateBy similarity1
    Binding sitei180SubstrateBy similarity1
    Binding sitei240SubstrateBy similarity1
    Binding sitei276SubstrateBy similarity1
    Binding sitei280Substrate; via amide nitrogenBy similarity1
    Binding sitei300Substrate; via amide nitrogenBy similarity1
    Binding sitei309SubstrateBy similarity1
    Binding sitei326SubstrateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiR-MMU-2022854 Keratan sulfate biosynthesis
    R-MMU-4085001 Sialic acid metabolism
    R-MMU-977068 Termination of O-glycan biosynthesis
    UniPathwayiUPA00378

    Protein family/group databases

    CAZyiGT29 Glycosyltransferase Family 29

    Chemistry databases

    SwissLipidsiSLP:000001389

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2 (EC:2.4.99.41 Publication)
    Short name:
    Alpha 2,3-ST 2
    Short name:
    Beta-galactoside alpha-2,3-sialyltransferase 2
    Alternative name(s):
    Gal-NAc6S
    Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
    ST3Gal II
    Short name:
    ST3GalII
    ST3GalA.2
    Sialyltransferase 4B
    Short name:
    SIAT4-B
    Gene namesi
    Name:St3gal2
    Synonyms:Siat4b, Siat5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:99427 St3gal2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 6CytoplasmicSequence analysis6
    Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini28 – 350LumenalSequence analysisAdd BLAST323

    Keywords - Cellular componenti

    Golgi apparatus, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001492591 – 350CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2Add BLAST350

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi70 ↔ 75By similarity
    Disulfide bondi72 ↔ 149By similarity
    Glycosylationi92N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi152 ↔ 291By similarity
    Glycosylationi211N-linked (GlcNAc...) asparagineSequence analysis1

    Post-translational modificationi

    The soluble form derives from the membrane form by proteolytic processing.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ11204
    PaxDbiQ11204
    PRIDEiQ11204

    PTM databases

    PhosphoSitePlusiQ11204

    Expressioni

    Tissue specificityi

    Strongly expressed in brain and liver and to a lesser extent in heart and kidney. Scarcely detectable in lung, pancreas, spleen and submaxillary gland.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000031749
    ExpressionAtlasiQ11204 baseline and differential
    GenevisibleiQ11204 MM

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000034197

    Structurei

    3D structure databases

    ProteinModelPortaliQ11204
    SMRiQ11204
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 29 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG2692 Eukaryota
    ENOG410XT8P LUCA
    GeneTreeiENSGT00760000119095
    HOGENOMiHOG000126811
    HOVERGENiHBG054227
    InParanoidiQ11204
    KOiK03368
    OMAiCACRRCM
    OrthoDBiEOG091G08DE
    TreeFamiTF354325

    Family and domain databases

    Gene3Di3.90.1480.20, 1 hit
    InterProiView protein in InterPro
    IPR001675 Glyco_trans_29
    IPR038578 GT29-like_sf
    IPR012163 Sialyl_trans
    PfamiView protein in Pfam
    PF00777 Glyco_transf_29, 1 hit
    PIRSFiPIRSF005557 Sialyl_trans, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q11204-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKCSLRVWFL SMAFLLVFIM SLLFTYSHHS MATLPYLDSG ALGGTHRVKL
    60 70 80 90 100
    VPGYSGLQRL GKEGLLGRNC ACSRCMGDAS TSEWFDSHFD GNISPVWTRD
    110 120 130 140 150
    NMNLPPDVQR WWMMLQPQFK SHNTNEVLEK LFQIVPGENP YRFRDPQQCR
    160 170 180 190 200
    RCAVVGNSGN LRGSGYGQEV DSHNFIMRMN QAPTVGFEKD VGSRTTHHFM
    210 220 230 240 250
    YPESAKNLPA NVSFVLVPFK ALDLMWIASA LSTGQIRFTY APVKSFLRVD
    260 270 280 290 300
    KEKVQIYNPA FFKYIHDRWT EHHGRYPSTG MLVLFFALHV CDEVNVYGFG
    310 320 330 340 350
    ADSRGNWHHY WENNRYAGEF RKTGVHDADF EAHIIDMLAK ASKIEVYRGN
    Length:350
    Mass (Da):40,130
    Last modified:July 27, 2011 - v2
    Checksum:i3C59FA39E6A03E4D
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti309 – 310HY → RH in CAA54294 (PubMed:8144500).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X76989 mRNA Translation: CAA54294.1
    AK053827 mRNA Translation: BAC35543.1
    AK131653 mRNA Translation: BAE20742.1
    CH466525 Genomic DNA Translation: EDL11478.1
    BC066064 mRNA Translation: AAH66064.1
    CCDSiCCDS22667.1
    PIRiA54420
    RefSeqiNP_033205.2, NM_009179.3
    XP_006530849.1, XM_006530786.2
    XP_006530850.1, XM_006530787.2
    XP_006530851.1, XM_006530788.1
    XP_017168113.1, XM_017312624.1
    UniGeneiMm.200388

    Genome annotation databases

    EnsembliENSMUST00000034197; ENSMUSP00000034197; ENSMUSG00000031749
    GeneIDi20444
    KEGGimmu:20444
    UCSCiuc009nlk.2 mouse

    Similar proteinsi

    Entry informationi

    Entry nameiSIA4B_MOUSE
    AccessioniPrimary (citable) accession number: Q11204
    Secondary accession number(s): Q8BPL0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 27, 2011
    Last modified: March 28, 2018
    This is version 134 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health