Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1

Gene

ST3GAL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

It may be responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found on sugar chains O-linked to Thr or Ser and also as a terminal sequence on certain gangliosides. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051SubstrateBy similarity
Binding sitei147 – 1471SubstrateBy similarity
Binding sitei170 – 1701SubstrateBy similarity
Binding sitei230 – 2301SubstrateBy similarity
Binding sitei266 – 2661SubstrateBy similarity
Binding sitei270 – 2701Substrate; via amide nitrogenBy similarity
Binding sitei290 – 2901Substrate; via amide nitrogenBy similarity
Binding sitei299 – 2991SubstrateBy similarity
Binding sitei316 – 3161SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS00250-MONOMER.
BRENDAi2.4.99.4. 2681.
2.4.99.6. 2681.
ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
R-HSA-4085001. Sialic acid metabolism.
R-HSA-977068. Termination of O-glycan biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 (EC:2.4.99.4)
Short name:
Alpha 2,3-ST 1
Short name:
Beta-galactoside alpha-2,3-sialyltransferase 1
Alternative name(s):
Gal-NAc6S
Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
SIATFL
ST3Gal I
Short name:
ST3GalI
ST3GalA.1
ST3O
Sialyltransferase 4A
Short name:
SIAT4-A
Gene namesi
Name:ST3GAL1
Synonyms:SIAT4, SIAT4A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:10862. ST3GAL1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3421Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini35 – 340306LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • Golgi cisterna membrane Source: UniProtKB-SubCell
  • Golgi membrane Source: Reactome
  • integral component of Golgi membrane Source: InterPro
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35764.

Polymorphism and mutation databases

BioMutaiST3GAL1.
DMDMi1705559.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1PRO_0000149253Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi59 ↔ 64By similarity
Disulfide bondi61 ↔ 139By similarity
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence analysis
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence analysis
Disulfide bondi142 ↔ 281By similarity
Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence analysis
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ11201.
MaxQBiQ11201.
PaxDbiQ11201.
PeptideAtlasiQ11201.
PRIDEiQ11201.

PTM databases

PhosphoSiteiQ11201.

Expressioni

Tissue specificityi

Expressed in several tissues. Highest expression in lung, liver, skeletal muscle, kidney, pancreas, spleen and placenta.

Gene expression databases

BgeeiENSG00000008513.
CleanExiHS_ST3GAL1.
ExpressionAtlasiQ11201. baseline and differential.
GenevisibleiQ11201. HS.

Organism-specific databases

HPAiHPA040466.

Interactioni

Protein-protein interaction databases

BioGridi112375. 14 interactions.
IntActiQ11201. 1 interaction.
STRINGi9606.ENSP00000318445.

Chemistry

BindingDBiQ11201.

Structurei

3D structure databases

ProteinModelPortaliQ11201.
SMRiQ11201. Positions 56-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00760000119095.
HOGENOMiHOG000126811.
HOVERGENiHBG054227.
InParanoidiQ11201.
KOiK00780.
OMAiTWFPKQM.
OrthoDBiEOG091G08DE.
PhylomeDBiQ11201.
TreeFamiTF354325.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q11201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTLRKRTLK VLTFLVLFIF LTSFFLNYSH TMVATTWFPK QMVLELSENL
60 70 80 90 100
KRLIKHRPCT CTHCIGQRKL SAWFDERFNQ TMQPLLTAQN ALLEDDTYRW
110 120 130 140 150
WLRLQREKKP NNLNDTIKEL FRVVPGNVDP MLEKRSVGCR RCAVVGNSGN
160 170 180 190 200
LRESSYGPEI DSHDFVLRMN KAPTAGFEAD VGTKTTHHLV YPESFRELGD
210 220 230 240 250
NVSMILVPFK TIDLEWVVSA ITTGTISHTY IPVPAKIRVK QDKILIYHPA
260 270 280 290 300
FIKYVFDNWL QGHGRYPSTG ILSVIFSMHV CDEVDLYGFG ADSKGNWHHY
310 320 330 340
WENNPSAGAF RKTGVHDADF ESNVTATLAS INKIRIFKGR
Length:340
Mass (Da):39,075
Last modified:October 1, 1996 - v1
Checksum:iA3E81D9C85446843
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121L → V in AAA36612 (PubMed:7655169).Curated
Sequence conflicti86 – 861L → V in AAC17874 (PubMed:10504389).Curated
Sequence conflicti219 – 2191S → R in AAC17874 (PubMed:10504389).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti111 – 1111N → S.
Corresponds to variant rs116342938 [ dbSNP | Ensembl ].
VAR_049225

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29555 mRNA. Translation: AAA36612.1.
L13972 mRNA. Translation: AAC37574.1.
AF059321 mRNA. Translation: AAC17874.1.
BC018357 mRNA. Translation: AAH18357.1.
CCDSiCCDS6373.1.
PIRiI54229.
RefSeqiNP_003024.1. NM_003033.3.
NP_775479.1. NM_173344.2.
XP_005251082.1. XM_005251025.4.
XP_006716680.1. XM_006716617.1.
XP_011515527.1. XM_011517225.1.
UniGeneiHs.374257.

Genome annotation databases

EnsembliENST00000399640; ENSP00000414073; ENSG00000008513.
ENST00000521180; ENSP00000428540; ENSG00000008513.
ENST00000522652; ENSP00000430515; ENSG00000008513.
GeneIDi6482.
KEGGihsa:6482.
UCSCiuc003yuk.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST3Gal I

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29555 mRNA. Translation: AAA36612.1.
L13972 mRNA. Translation: AAC37574.1.
AF059321 mRNA. Translation: AAC17874.1.
BC018357 mRNA. Translation: AAH18357.1.
CCDSiCCDS6373.1.
PIRiI54229.
RefSeqiNP_003024.1. NM_003033.3.
NP_775479.1. NM_173344.2.
XP_005251082.1. XM_005251025.4.
XP_006716680.1. XM_006716617.1.
XP_011515527.1. XM_011517225.1.
UniGeneiHs.374257.

3D structure databases

ProteinModelPortaliQ11201.
SMRiQ11201. Positions 56-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112375. 14 interactions.
IntActiQ11201. 1 interaction.
STRINGi9606.ENSP00000318445.

Chemistry

BindingDBiQ11201.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteiQ11201.

Polymorphism and mutation databases

BioMutaiST3GAL1.
DMDMi1705559.

Proteomic databases

EPDiQ11201.
MaxQBiQ11201.
PaxDbiQ11201.
PeptideAtlasiQ11201.
PRIDEiQ11201.

Protocols and materials databases

DNASUi6482.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000399640; ENSP00000414073; ENSG00000008513.
ENST00000521180; ENSP00000428540; ENSG00000008513.
ENST00000522652; ENSP00000430515; ENSG00000008513.
GeneIDi6482.
KEGGihsa:6482.
UCSCiuc003yuk.3. human.

Organism-specific databases

CTDi6482.
GeneCardsiST3GAL1.
HGNCiHGNC:10862. ST3GAL1.
HPAiHPA040466.
MIMi607187. gene.
neXtProtiNX_Q11201.
PharmGKBiPA35764.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00760000119095.
HOGENOMiHOG000126811.
HOVERGENiHBG054227.
InParanoidiQ11201.
KOiK00780.
OMAiTWFPKQM.
OrthoDBiEOG091G08DE.
PhylomeDBiQ11201.
TreeFamiTF354325.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:HS00250-MONOMER.
BRENDAi2.4.99.4. 2681.
2.4.99.6. 2681.
ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
R-HSA-4085001. Sialic acid metabolism.
R-HSA-977068. Termination of O-glycan biosynthesis.

Miscellaneous databases

ChiTaRSiST3GAL1. human.
GenomeRNAii6482.
PROiQ11201.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000008513.
CleanExiHS_ST3GAL1.
ExpressionAtlasiQ11201. baseline and differential.
GenevisibleiQ11201. HS.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIA4A_HUMAN
AccessioniPrimary (citable) accession number: Q11201
Secondary accession number(s): O60677, Q9UN51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.