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Protein

CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1

Gene

ST3GAL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found on sugar chains O-linked to Thr or Ser and also as a terminal sequence on certain gangliosides. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.By similarity

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei105SubstrateBy similarity1
Binding sitei147SubstrateBy similarity1
Binding sitei170SubstrateBy similarity1
Binding sitei230SubstrateBy similarity1
Binding sitei266SubstrateBy similarity1
Binding sitei270Substrate; via amide nitrogenBy similarity1
Binding sitei290Substrate; via amide nitrogenBy similarity1
Binding sitei299SubstrateBy similarity1
Binding sitei316SubstrateBy similarity1

GO - Molecular functioni

  • beta-galactoside (CMP) alpha-2,3-sialyltransferase activity Source: CACAO

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS00250-MONOMER.
BRENDAi2.4.99.4. 2681.
2.4.99.6. 2681.
ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
R-HSA-4085001. Sialic acid metabolism.
R-HSA-977068. Termination of O-glycan biosynthesis.
SIGNORiQ11201.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 (EC:2.4.99.4By similarity)
Short name:
Alpha 2,3-ST 1
Short name:
Beta-galactoside alpha-2,3-sialyltransferase 1
Alternative name(s):
Gal-NAc6S
Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
SIATFL
ST3Gal I
Short name:
ST3GalI
ST3GalA.1
ST3O
Sialyltransferase 4A
Short name:
SIAT4-A
Gene namesi
Name:ST3GAL1
Synonyms:SIAT4, SIAT4A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000008513.14.
HGNCiHGNC:10862. ST3GAL1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 34Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini35 – 340LumenalSequence analysisAdd BLAST306

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi6482.
OpenTargetsiENSG00000008513.
PharmGKBiPA35764.

Chemistry databases

ChEMBLiCHEMBL3596074.

Polymorphism and mutation databases

BioMutaiST3GAL1.
DMDMi1705559.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492531 – 340CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1Add BLAST340

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi59 ↔ 64By similarity
Disulfide bondi61 ↔ 139By similarity
Glycosylationi79N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi142 ↔ 281By similarity
Glycosylationi201N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi323N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ11201.
MaxQBiQ11201.
PaxDbiQ11201.
PeptideAtlasiQ11201.
PRIDEiQ11201.

PTM databases

PhosphoSitePlusiQ11201.

Expressioni

Tissue specificityi

Expressed in several tissues. Highest expression in lung, liver, skeletal muscle, kidney, pancreas, spleen and placenta.

Gene expression databases

BgeeiENSG00000008513.
CleanExiHS_ST3GAL1.
ExpressionAtlasiQ11201. baseline and differential.
GenevisibleiQ11201. HS.

Organism-specific databases

HPAiHPA040466.

Interactioni

Protein-protein interaction databases

BioGridi112375. 16 interactors.
IntActiQ11201. 1 interactor.
STRINGi9606.ENSP00000318445.

Chemistry databases

BindingDBiQ11201.

Structurei

3D structure databases

ProteinModelPortaliQ11201.
SMRiQ11201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00760000119095.
HOGENOMiHOG000126811.
HOVERGENiHBG054227.
InParanoidiQ11201.
KOiK00780.
OMAiTWFPKQM.
OrthoDBiEOG091G08DE.
PhylomeDBiQ11201.
TreeFamiTF354325.

Family and domain databases

InterProiView protein in InterPro
IPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
PfamiView protein in Pfam
PF00777. Glyco_transf_29. 1 hit.
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q11201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTLRKRTLK VLTFLVLFIF LTSFFLNYSH TMVATTWFPK QMVLELSENL
60 70 80 90 100
KRLIKHRPCT CTHCIGQRKL SAWFDERFNQ TMQPLLTAQN ALLEDDTYRW
110 120 130 140 150
WLRLQREKKP NNLNDTIKEL FRVVPGNVDP MLEKRSVGCR RCAVVGNSGN
160 170 180 190 200
LRESSYGPEI DSHDFVLRMN KAPTAGFEAD VGTKTTHHLV YPESFRELGD
210 220 230 240 250
NVSMILVPFK TIDLEWVVSA ITTGTISHTY IPVPAKIRVK QDKILIYHPA
260 270 280 290 300
FIKYVFDNWL QGHGRYPSTG ILSVIFSMHV CDEVDLYGFG ADSKGNWHHY
310 320 330 340
WENNPSAGAF RKTGVHDADF ESNVTATLAS INKIRIFKGR
Length:340
Mass (Da):39,075
Last modified:October 1, 1996 - v1
Checksum:iA3E81D9C85446843
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12L → V in AAA36612 (PubMed:7655169).Curated1
Sequence conflicti86L → V in AAC17874 (PubMed:10504389).Curated1
Sequence conflicti219S → R in AAC17874 (PubMed:10504389).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049225111N → S. Corresponds to variant dbSNP:rs116342938Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29555 mRNA. Translation: AAA36612.1.
L13972 mRNA. Translation: AAC37574.1.
AF059321 mRNA. Translation: AAC17874.1.
BC018357 mRNA. Translation: AAH18357.1.
CCDSiCCDS6373.1.
PIRiI54229.
RefSeqiNP_003024.1. NM_003033.3.
NP_775479.1. NM_173344.2.
XP_005251082.1. XM_005251025.4.
XP_006716680.1. XM_006716617.1.
XP_011515527.1. XM_011517225.1.
XP_016869225.1. XM_017013736.1.
XP_016869226.1. XM_017013737.1.
UniGeneiHs.374257.

Genome annotation databases

EnsembliENST00000399640; ENSP00000414073; ENSG00000008513.
ENST00000521180; ENSP00000428540; ENSG00000008513.
ENST00000522652; ENSP00000430515; ENSG00000008513.
GeneIDi6482.
KEGGihsa:6482.
UCSCiuc003yuk.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSIA4A_HUMAN
AccessioniPrimary (citable) accession number: Q11201
Secondary accession number(s): O60677, Q9UN51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families