Reviewed, UniProtKB/Swiss-Prot Q11190 (ETFD_CAEEL)

Names and origin

Protein names

Recommended name:
    Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
      Short name=ETF-ubiquinone oxidoreductase
      Short name=ETF-QO
    EC=1.5.5.1
Alternative name(s):
    Electron-transferring-flavoprotein dehydrogenase
      Short name=ETF dehydrogenase
    Lethal protein 721

Gene names

Name:	let-721
ORF Names:	C05D11.12

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Accepts electrons from ETF and reduces ubiquinone By similarity.
Catalytic activity	Reduced electron-transferring flavoprotein + ubiquinone = electron-transferring flavoprotein + ubiquinol.
Cofactor	Binds 1 4Fe-4S cluster By similarity. FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion inner membrane By similarity.
Sequence similarities	Belongs to the ETF-QO/fixC family. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	4Fe-4S FAD Flavoprotein Iron Iron-sulfur Metal-binding Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	body morphogenesis Inferred from mutant phenotype. Source: WormBase electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron-transferring-flavoprotein dehydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 597		Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial		PRO_0000008665
Regions
	Transmembrane	437 – 457	21	Potential
	Domain	557 – 586	30	4Fe-4S ferredoxin-type
	Nucleotide binding	53 – 67	15	FAD Potential
Sites
	Metal binding	540	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	566	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	569	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	572	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q11190-1 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: C0FF09F185A017FB	FASTA	597	65,335
	10 20 30 40 50 60 MRISGVTLFR VSSQLRNVVN GQWTTTHYTV KDRSTDPRWK DVDLARESDV YDVVIVGGGP 70 80 90 100 110 120 SGLSAAIRLR QLAEKAQKEL RVCVVEKASV IGGHTLSGAV IETRALDELI PNWKELGAPV 130 140 150 160 170 180 YQQVTSESIA ILTESGRIPV PVLPGVPLAN HGNYIVRLGK VVQWLGEQAE AAGVEVWPEI 190 200 210 220 230 240 AASEVLYNED GSVKGIATSD VGIGKDGAPK DGFARGMEFH AKCTIFAEGC RGHLSKQVLD 250 260 270 280 290 300 KFDLRTHAMT YGIGLKELWE IDPAKHRPGY VEHTMGWPLN VDQYGGSFLY HIEDQGQPLV 310 320 330 340 350 360 SVGFVVALDY ANPNLNPYKE FQKYKTHPSI SKQLEGGKRI GYGARALNEG GFQSIPKLHF 370 380 390 400 410 420 PGGCLVGCSA GFLNVAKLKG THNAMKSGMV AAESIFEDIQ QKGEDVQTID PATYDKNIRD 430 440 450 460 470 480 TYVVKELKAT RNIRPSFNTS LGYIGGLIYS GIFYVFGRGI EPWTLGHGKK DNEKLIPVKD 490 500 510 520 530 540 AKEIDYPKPD GKLTFDLLTS VSLTGTNHTE DQPAHLTLKN DQVPLDVNLA VYGGPEARFC 550 560 570 580 590 PAGVYEFVPS EADESKKRLQ INAQNCIHCK TCDIKDPQQN INWVTPEGGG GPKYEGM

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00048 Genomic DNA. Translation: AAB53835.2.
PIR	D88483.
RefSeq	NP_498415.2.
UniGene	Cel.17705
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	C05D11.12. Caenorhabditis elegans. [Contig view]
GeneID	175916.
KEGG	cel:C05D11.12.
Organism-specific databases
WormBase	WBGene00002855. let-721.
WormPep	C05D11.12. CE29662. [WorfDB]
Phylogenomic databases
OMA	Q11190. KGIHTAM.
Enzyme and pathway databases
BRENDA	1.5.5.1. 672.
Gene expression databases
ArrayExpress	Q11190.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR007859. ETFD_OxRdtase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. [Graphical view]
Pfam	PF05187. ETF_QO. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PROSITE	PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	890294.

Entry information

Entry name

ETFD_CAEEL

Accession

Primary (citable) accession number: Q11190

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 27, 2002
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project