Q11174 (CHIT_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable endochitinase
EC=3.2.1.14

Gene names

Name:	cht-1
ORF Names:	C04F6.3

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. Contains 2 chitin-binding type-2 domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Domain	Repeat
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	Probable endochitinase		PRO_0000077049
Regions
	Domain	478 – 534	57	Chitin-binding type-2 1
	Domain	563 – 617	55	Chitin-binding type-2 2
	Compositional bias	435 – 478	44	Thr-rich
Sites
	Active site	179	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	310	1	N-linked (GlcNAc...) Ref.3 Ref.4
	Disulfide bond	511 ↔ 524		By similarity
	Disulfide bond	594 ↔ 607		By similarity
Experimental info
	Sequence conflict	138	1	W → C in AAB81847. Ref.2
	Sequence conflict	163 – 165	3	ITF → TTS in AAB81847. Ref.2
	Sequence conflict	176	1	I → L in AAB81847. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q11174 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: DDA1D2AAAC0E54DA	FASTA	617	66,857
	10 20 30 40 50 60 MLLGKFLLVA SFILPIAYTW TGATIRNHPA DVVAARNKIT SRSVARSEPT NSYIRPCYFT 70 80 90 100 110 120 NWAQYRQGRA KFVPEDYTPG LCTHILFAFG WMNADYTVRA YDPADLPNDW AGEGMYRRVN 130 140 150 160 170 180 KLKVTDTQLK TLLSFGGWSF GTALFQGMAA SSASRKVFID SAITFVRTWG FDGIDIDWEY 190 200 210 220 230 240 PSGATDMANY VALVKELKAA CESEAGSTGK DRLLVTAAVA AGPATIDAGY DIPNLAPNFD 250 260 270 280 290 300 FILLMSYDFF GAWASLVGFN SPLYATTELP AEWNGWNVDS SARYWNQKGM PKEKIIVGMP 310 320 330 340 350 360 TYGRGWTLNN ASAINPGTSG SPAKITQYVQ EAGVGAYFEF CEMLANGATR YWDSQSQVPY 370 380 390 400 410 420 LVQGNQWWSY DDEESFANKM AYVKREGYGG AFVWTLDFDD FNAGCSNSNG QLYPLISVIA 430 440 450 460 470 480 KELGGVIIPK KGGVTTAPTT VATTVTTGRP PMTSAVTTTT AATTTTTRAA TTTTASNTNV 490 500 510 520 530 540 CSGKSDGFYP NSNNCGLFVL CLSSKSYSMS CPSGLQYSAS LKYCTTSTAS GCSVTTTRAP 550 560 570 580 590 600 TTTTKSAPTV TTTTRAPTTT TPAFKCTKDG FFGVPSDCLK FIRCVNGISY NFECPNGLSF 610 HADTMMCDRP DPSKCAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Chitinases are a multi-gene family in Aedes, Anopheles and Drosophila." de la Vega H., Specht C.A., Liu Y., Robbins P.W. Insect Mol. Biol. 7:233-239(1998) [PubMed: 9662472] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-178.
[3]	"Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins." Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T. Nat. Biotechnol. 21:667-672(2003) [PubMed: 12754521] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-310, MASS SPECTROMETRY. Strain: Bristol N2.
[4]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-310, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080344 Genomic DNA. Translation: CCD63028.1. AF026152 Genomic DNA. Translation: AAB81847.1.
PIR	T15408. T37249.
RefSeq	NP_508588.1. NM_076187.3.
UniGene	Cel.17326.
3D structure databases
ProteinModelPortal	Q11174.
SMR	Q11174. Positions 54-423, 564-617.
ModBase	Search...
Protein-protein interaction databases
STRING	Q11174.
Protein family/group databases
CAZy	CBM14. Carbohydrate-Binding Module Family 14. GH18. Glycoside Hydrolase Family 18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C04F6.3.1; C04F6.3.1; C04F6.3. C04F6.3.2; C04F6.3.2; C04F6.3.
GeneID	180628.
KEGG	cel:C04F6.3.
UCSC	C04F6.3.1. c. elegans.
Organism-specific databases
CTD	180628.
WormBase	C04F6.3; CE03923; WBGene00000503; cht-1.
Phylogenomic databases
eggNOG	meNOG04095.
GeneTree	EMGT00050000002461.
HOGENOM	HBG443716.
InParanoid	Q11174.
OMA	PGQDTFC.
PhylomeDB	Q11174.
Gene expression databases
ArrayExpress	Q11174.
Family and domain databases
InterPro	IPR002557. Chitin-bd_dom. IPR011583. Chitinase_II. IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.170.140.10. Chitin-bd_dom. 2 hits. G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
KO	K01183.
Pfam	PF01607. CBM_14. 2 hits. PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
SMART	SM00494. ChtBD2. 2 hits. SM00636. Glyco_18. 1 hit. [Graphical view]
SUPFAM	SSF57625. Chitin_bind_PerA. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS50940. CHIT_BIND_II. 2 hits. PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	910182.

Entry information

Entry name

CHIT_CAEEL

Accession

Primary (citable) accession number: Q11174
Secondary accession number(s): O17321

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project