Q11141 (P5CR_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyrroline-5-carboxylate reductase
Short name=P5C reductase
Short name=P5CR
EC=1.5.1.2

Gene names

Name:	proC
Ordered Locus Names:	Rv0500, MT0520
ORF Names:	MTCY20G9.26

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE proline biosynthetic process Inferred from direct assay. Source: MTBBASE protein homooligomerization Inferred from physical interaction. Source: MTBBASE
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	magnesium ion binding Inferred from direct assay. Source: MTBBASE manganese ion binding Inferred from direct assay. Source: MTBBASE nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro pyrroline-5-carboxylate reductase activity Inferred from direct assay. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Pyrroline-5-carboxylate reductase		PRO_0000187294

Sequences

	Sequence		Length	Mass (Da)
	Q11141 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F95C3F407BE5408F	FASTA	295	30,172
	10 20 30 40 50 60 MLFGMARIAI IGGGSIGEAL LSGLLRAGRQ VKDLVVAERM PDRANYLAQT YSVLVTSAAD 70 80 90 100 110 120 AVENATFVVV AVKPADVEPV IADLANATAA AENDSAEQVF VTVVAGITIA YFESKLPAGT 130 140 150 160 170 180 PVVRAMPNAA ALVGAGVTAL AKGRFVTPQQ LEEVSALFDA VGGVLTVPES QLDAVTAVSG 190 200 210 220 230 240 SGPAYFFLLV EALVDAGVGV GLSRQVATDL AAQTMAGSAA MLLERMEQDQ GGANGELMGL 250 260 270 280 290 RVDLTASRLR AAVTSPGGTT AAALRELERG GFRMAVDAAV QAAKSRSEQL RITPE

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842573 Genomic DNA. Translation: CAB00926.1. AE000516 Genomic DNA. Translation: AAK44743.1.
PIR	G70745.
RefSeq	NP_215014.1. NC_000962.2. NP_334929.1. NC_002755.2.
3D structure databases
ProteinModelPortal	Q11141.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000000256; EBMYCP00000000256; EBMYCG00000000256. EBMYCT00000070475; EBMYCP00000068534; EBMYCG00000070470.
GeneID	887256. 923905.
GenomeReviews	Gene locus MT0520 in contig AE000516_GR. Gene locus Rv0500 in contig AL123456_GR.
KEGG	mtc:MT0520. mtu:Rv0500.
PATRIC	18122852. VBIMycTub22151_0560.
TIGR	MT0520.
Organism-specific databases
TubercuList	Rv0500.
Phylogenomic databases
GeneTree	EBGT00050000017740.
HOGENOM	HBG726602.
OMA	INSGRIT.
PhylomeDB	Q11141.
ProtClustDB	PRK11880.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR004455. NADP_OxRdtase_F420. IPR000304. Pyrroline-COOH_reductase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00286.
PANTHER	PTHR11645. P5CR. 1 hit.
Pfam	PF03807. F420_oxidored. 1 hit. [Graphical view]
PIRSF	PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00112. ProC. 1 hit.
PROSITE	PS00521. P5CR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P5CR_MYCTU

Accession

Primary (citable) accession number: Q11141

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program