Q11130 (FUT7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-(1,3)-fucosyltransferase
EC=2.4.1.-

Alternative name(s):
Fucosyltransferase 7
Fucosyltransferase VII
Short name=Fuc-TVII
Short name=FucT-VII
Galactoside 3-L-fucosyltransferase
Selectin ligand synthase

Gene names

Name:

FUT7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May catalyze alpha-1,3 glycosidic linkages involved in the expression of sialyl Lewis X antigens.
Catalytic activity	GDP-L-fucose + alpha-2,3-Neu-N-acetyl-1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R = GDP + alpha-2,3-Neu-N-acetyl-1,4-beta-D-galactosyl-(alpha-1,3-L-fucosyl)-N-acetyl-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
Tissue specificity	Leukocytic/myeloid lineage cells.
Sequence similarities	Belongs to the glycosyltransferase 10 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation Inferred from electronic annotation. Source: Compara L-fucose catabolic process Non-traceable author statement PubMed 11698403. Source: UniProtKB leukocyte migration involved in immune response Inferred from electronic annotation. Source: Compara protein glycosylation Traceable author statement PubMed 11698403. Source: UniProtKB
Cellular_component	Golgi apparatus Traceable author statement PubMed 11698403. Source: UniProtKB Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	alpha-(1->3)-fucosyltransferase activity Traceable author statement PubMed 11698403. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Alpha-(1,3)-fucosyltransferase		PRO_0000221113
Regions
	Topological domain	1 – 14	14	Cytoplasmic Potential
	Transmembrane	15 – 36	22	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	37 – 342	306	Lumenal Potential
Amino acid modifications
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	291	1	N-linked (GlcNAc...) Potential
	Disulfide bond	68 ↔ 76		Ref.8
	Disulfide bond	211 ↔ 214		Ref.8
	Disulfide bond	318 ↔ 321		Ref.8
Experimental info
	Sequence conflict	161 – 162	2	GP → A in AAA56869. Ref.1
	Sequence conflict	304 – 305	2	RL → SV in AAA56869. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q11130 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D31BFF90DD64DFAB	FASTA	342	39,239
	10 20 30 40 50 60 MNNAGHGPTR RLRGLGVLAG VALLAALWLL WLLGSAPRGT PAPQPTITIL VWHWPFTDQP 70 80 90 100 110 120 PELPSDTCTR YGIARCHLSA NRSLLASADA VVFHHRELQT RRSHLPLAQR PRGQPWVWAS 130 140 150 160 170 180 MESPSHTHGL SHLRGIFNWV LSYRRDSDIF VPYGRLEPHW GPSPPLPAKS RVAAWVVSNF 190 200 210 220 230 240 QERQLRARLY RQLAPHLRVD VFGRANGRPL CASCLVPTVA QYRFYLSFEN SQHRDYITEK 250 260 270 280 290 300 FWRNALVAGT VPVVLGPPRA TYEAFVPADA FVHVDDFGSA RELAAFLTGM NESRYQRFFA 310 320 330 340 WRDRLRVRLF TDWRERFCAI CDRYPHLPRS QVYEDLEGWF QA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a cDNA encoding a novel human leukocyte alpha-1,3-fucosyltransferase capable of synthesizing the sialyl Lewis x determinant." Natsuka S., Gersten K.M., Zenita K., Kannagi R., Lowe J.B. J. Biol. Chem. 269:16789-16794(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of a cDNA encoding a novel human leukocyte alpha-1,3-fucosyltransferase capable of synthesizing the sialyl Lewis x determinant." Natsuka S., Gersten K.M., Zenita K., Kannagi R., Lowe J.B. J. Biol. Chem. 269:20806-20806(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Expression cloning of a novel alpha 1,3-fucosyltransferase that is involved in biosynthesis of the sialyl Lewis x carbohydrate determinants in leukocytes." Sasaki K., Kurata K., Funayama K., Nagata M., Watanabe E., Ohta S., Hanai N., Nishi T. J. Biol. Chem. 269:14730-14737(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The human selectin-ligand synthase (hFuc-T VII) gene structure and characterization of the promoter." Hiraiwa N., Hiraiwa M., Kannagi R. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[6]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Mammary gland.
[8]	"Neighboring cysteine residues in human fucosyltransferase VII are engaged in disulfide bridges, forming small loop structures." de Vries T., Yen T.Y., Joshi R.K., Storm J., van Den Eijnden D.H., Knegtel R.M.A., Bunschoten H., Joziasse D.H., Macher B.A. Glycobiology 11:423-432(2001) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BONDS.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Fucosyltransferase 7

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78031 mRNA. Translation: CAA54962.1. U11282 mRNA. Translation: AAA20468.1. U08112 mRNA. Translation: AAA56869.1. AB012668 Genomic DNA. Translation: BAA32819.1. AK313124 mRNA. Translation: BAG35944.1. AL807752 Genomic DNA. Translation: CAI12771.1. BC074746 mRNA. Translation: AAH74746.2. BC086312 mRNA. Translation: AAH86312.1.
IPI	IPI00009217.
PIR	A54057.
RefSeq	NP_004470.1. NM_004479.3.
UniGene	Hs.457.
3D structure databases
ProteinModelPortal	Q11130.
SMR	Q11130. Positions 167-308.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000318142.
Protein family/group databases
CAZy	GT10. Glycosyltransferase Family 10.
PTM databases
PhosphoSite	Q11130.
Polymorphism databases
DMDM	1730137.
Proteomic databases
PaxDb	Q11130.
PRIDE	Q11130.
Protocols and materials databases
DNASU	2529.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000314412; ENSP00000318142; ENSG00000180549.
GeneID	2529.
KEGG	hsa:2529.
UCSC	uc004ckq.2. human.
Organism-specific databases
CTD	2529.
GeneCards	GC09M139924.
HGNC	HGNC:4018. FUT7.
MIM	602030. gene.
neXtProt	NX_Q11130.
PharmGKB	PA28434.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG252779.
HOGENOM	HOG000045583.
HOVERGEN	HBG000274.
InParanoid	Q11130.
KO	K07635.
OMA	HHRELQT.
OrthoDB	EOG4R7VBK.
PhylomeDB	Q11130.
Enzyme and pathway databases
BRENDA	2.4.1.65. 2681.
UniPathway	UPA00378.
Gene expression databases
Bgee	Q11130.
CleanEx	HS_FUT7.
Genevestigator	Q11130.
GermOnline	ENSG00000180549. Homo sapiens.
Family and domain databases
InterPro	IPR001503. Glyco_trans_10. [Graphical view]
PANTHER	PTHR11929. PTHR11929. 1 hit.
Pfam	PF00852. Glyco_transf_10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	2529.
NextBio	9967.
SOURCE	Search...

Entry information

Entry name

FUT7_HUMAN

Accession

Primary (citable) accession number: Q11130
Secondary accession number(s): B2R7U7, Q6DK54

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.