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Protein

Alpha-galactosidase

Gene

MEL

Organism
Saccharomyces mikatae (Yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72SubstrateBy similarity1
Binding sitei73SubstrateBy similarity1
Binding sitei147SubstrateBy similarity1
Active sitei149NucleophileBy similarity1
Binding sitei205SubstrateBy similarity1
Active sitei209Proton donorBy similarity1
Binding sitei251SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH27 Glycoside Hydrolase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactoside galactohydrolase
MELj
Melibiase
Gene namesi
Name:MEL
OrganismiSaccharomyces mikatae (Yeast)
Taxonomic identifieri114525 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000000100719 – 471Alpha-galactosidaseAdd BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 74By similarity
Glycosylationi82N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi121 ↔ 151By similarity
Glycosylationi175N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi221 ↔ 237By similarity
Disulfide bondi223 ↔ 230By similarity
Glycosylationi270N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi361N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi370N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi417N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi422N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi435N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi454N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ11129

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ11129
SMRiQ11129
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd14792 GH27, 1 hit
Gene3Di2.60.40.1180, 1 hit
3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR002241 Glyco_hydro_27
IPR000111 Glyco_hydro_27/36_CS
IPR013780 Glyco_hydro_b
IPR006215 Glyco_hydro_melibiase
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF16499 Melibiase_2, 1 hit
PRINTSiPR00740 GLHYDRLASE27
PR00748 MELIBIASE
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00512 ALPHA_GALACTOSIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q11129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYIYLFITA AAVTGALGSS PSYNGLGLTP QMGWDNWNTF ACDVSEQLLL
60 70 80 90 100
NTADRISEIG LKDLGYKYVI LDDCWSSGRN SNGTLVADKN KFPNGMDHVA
110 120 130 140 150
RHLHNNNFLF GMYSSAGEYT CAGYPGSLGH EQEDAEFFAR NGVDYLKYDN
160 170 180 190 200
CYNKGKFGTP ETSYKRYKAM SDALNKTGRP IFYSLCNWGQ DLTFYWGSDI
210 220 230 240 250
ANSWRMSGDI YPEFDRPDSR CPCDGDQYDC SYAGFHCSIM NILNKAAPMG
260 270 280 290 300
QNAGIGGWND LDNLEVGVGN LTDDEEKAHF SMWAMVKSPL IIGADVNHLK
310 320 330 340 350
ESSYSIYSQA SVIAINQDPK GVPATRVWRH YVSQTDKYGK GEIQLWSCPL
360 370 380 390 400
DNGDQVIALL NGSNKKRPMN ASLEDIFFDS YLGSEELSSS WDIYDLWANR
410 420 430 440 450
IDNTIASNIL KNNKVTNSSL YNATELSYKE GLSKNDTRLF GVQIGTVSPG
460 470
GLLNTTVPAH GVALYRLRRS R
Length:471
Mass (Da):52,378
Last modified:November 1, 1996 - v1
Checksum:i9FFF2FA03C03872A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95506 Genomic DNA Translation: CAA64760.1

Similar proteinsi

Entry informationi

Entry nameiMEL_SACMI
AccessioniPrimary (citable) accession number: Q11129
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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