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Reviewed, UniProtKB/Swiss-Prot Q11129 (MEL_SACMI)

Last modified June 16, 2009. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Alpha-D-galactoside galactohydrolase
    Melibiase
    MELj
Gene names
Name: MEL
OrganismSaccharomyces mikatae (Yeast)
Taxonomic identifier114525 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 27 family.

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Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionalpha-galactosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 By similarity
Chain19 – 471453Alpha-galactosidase
PRO_0000001007

Sites

Active site1491Nucleophile By similarity
Active site2091Proton donor By similarity

Amino acid modifications

Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation1751N-linked (GlcNAc...) Potential
Glycosylation2701N-linked (GlcNAc...) Potential
Glycosylation3611N-linked (GlcNAc...) Potential
Glycosylation3701N-linked (GlcNAc...) Potential
Glycosylation4171N-linked (GlcNAc...) Potential
Glycosylation4221N-linked (GlcNAc...) Potential
Glycosylation4351N-linked (GlcNAc...) Potential
Glycosylation4541N-linked (GlcNAc...) Potential
Disulfide bond42 ↔ 74 By similarity
Disulfide bond121 ↔ 151 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q11129-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9FFF2FA03C03872A

FASTA47152,378
        10         20         30         40         50         60 
MSYIYLFITA AAVTGALGSS PSYNGLGLTP QMGWDNWNTF ACDVSEQLLL NTADRISEIG 

        70         80         90        100        110        120 
LKDLGYKYVI LDDCWSSGRN SNGTLVADKN KFPNGMDHVA RHLHNNNFLF GMYSSAGEYT 

       130        140        150        160        170        180 
CAGYPGSLGH EQEDAEFFAR NGVDYLKYDN CYNKGKFGTP ETSYKRYKAM SDALNKTGRP 

       190        200        210        220        230        240 
IFYSLCNWGQ DLTFYWGSDI ANSWRMSGDI YPEFDRPDSR CPCDGDQYDC SYAGFHCSIM 

       250        260        270        280        290        300 
NILNKAAPMG QNAGIGGWND LDNLEVGVGN LTDDEEKAHF SMWAMVKSPL IIGADVNHLK 

       310        320        330        340        350        360 
ESSYSIYSQA SVIAINQDPK GVPATRVWRH YVSQTDKYGK GEIQLWSCPL DNGDQVIALL 

       370        380        390        400        410        420 
NGSNKKRPMN ASLEDIFFDS YLGSEELSSS WDIYDLWANR IDNTIASNIL KNNKVTNSSL 

       430        440        450        460        470 
YNATELSYKE GLSKNDTRLF GVQIGTVSPG GLLNTTVPAH GVALYRLRRS R

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References

[1]"Superfamily of alpha-galactosidase MEL genes of the Saccharomyces sensu stricto species complex."
Naumova E.S., Turakainen H., Naumov G.I., Korhola M.
Mol. Gen. Genet. 253:111-117(1996) [PubMed: 9003294] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: IFO 1816.

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Cross-references

Sequence databases

X95506 Genomic DNA. Translation: CAA64760.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH27. Glycoside Hydrolase Family 27.

Enzyme and pathway databases

BRENDA3.2.1.22. 278541.

Family and domain databases

InterProIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR006215. Glyco_hydro_melibiase.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PfamPF02065. Melibiase. 1 hit.
[Graphical view]
PRINTSPR00740. GLHYDRLASE27.
PR00748. MELIBIASE.
ProDomPD002572. Glyco_hydro_GHD. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMEL_SACMI
AccessionPrimary (citable) accession number: Q11129
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents